Experiment set1IT012 for Pseudomonas syringae pv. syringae B728a ΔmexB

KB with Acriflavine 1.25 ug/ml

Group: stress
Media: KB + Acriflavine (1.25 ug/ml)
Culturing: SyringaeB728a_mexBdelta_ML3, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
By: Tyler on 12/4/17
Media components: 10 g/L Bacto Peptone, 1.5 g/L Potassium phosphate dibasic, 15 g/L Glycerol, 0.6 g/L Magnesium sulfate

Specific Phenotypes

For 7 genes in this experiment

For stress Acriflavine in Pseudomonas syringae pv. syringae B728a ΔmexB

For stress Acriflavine across organisms

SEED Subsystems

Subsystem	#Specific
Biogenesis of cytochrome c oxidases	2
Multidrug Resistance Efflux Pumps	2
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	2
Transport of Iron	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Urea cycle and metabolism of amino groups
• Arginine and proline metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
putrescine degradation II	4	1	1
superpathway of ornithine degradation	8	4	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	5	1
superpathway of L-arginine and L-ornithine degradation	13	7	1