Experiment set1IT012 for Escherichia coli BW25113
D-Xylose carbon source
Group: carbon sourceMedia: M9 minimal media_noCarbon + D-Xylose (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 5.6 generations
By: Kelly on 6/19/2014
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride
Specific Phenotypes
For 6 genes in this experiment
For carbon source D-Xylose in Escherichia coli BW25113
For carbon source D-Xylose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Xylose utilization | 4 |
| Acetoin, butanediol metabolism | 1 |
| Branched-Chain Amino Acid Biosynthesis | 1 |
| Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pentose and glucuronate interconversions
- Fructose and mannose metabolism
- Valine, leucine and isoleucine biosynthesis
- Butanoate metabolism
- C5-Branched dibasic acid metabolism
- Pantothenate and CoA biosynthesis
- Biosynthesis of alkaloids derived from histidine and purine
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-xylose degradation I | 2 | 2 | 2 |
| pyruvate fermentation to (R)-acetoin II | 2 | 1 | 1 |
| xylitol degradation I | 2 | 1 | 1 |
| D-arabinitol degradation I | 2 | 1 | 1 |
| pyruvate fermentation to (S)-acetoin | 3 | 3 | 1 |
| pyruvate fermentation to (R)-acetoin I | 3 | 2 | 1 |
| L-valine biosynthesis | 4 | 4 | 1 |
| pyruvate fermentation to isobutanol (engineered) | 5 | 4 | 1 |
| superpathway of (R,R)-butanediol biosynthesis | 5 | 2 | 1 |
| L-isoleucine biosynthesis IV | 6 | 4 | 1 |
| superpathway of 2,3-butanediol biosynthesis | 6 | 3 | 1 |
| L-isoleucine biosynthesis I (from threonine) | 7 | 7 | 1 |
| L-isoleucine biosynthesis III | 7 | 4 | 1 |
| L-isoleucine biosynthesis II | 8 | 4 | 1 |
| superpathway of branched chain amino acid biosynthesis | 17 | 17 | 2 |
| superpathway of glucose and xylose degradation | 17 | 17 | 2 |
| superpathway of L-isoleucine biosynthesis I | 13 | 13 | 1 |
| superpathway of L-threonine metabolism | 18 | 16 | 1 |
| superpathway of pentose and pentitol degradation | 42 | 18 | 2 |