Experiment set1IT011 for Escherichia coli BW25113

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D-Xylose carbon source

Group: carbon source
Media: M9 minimal media_noCarbon + D-Xylose (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 5.6 generations
By: Kelly on 6/19/2014
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 6 genes in this experiment

For carbon source D-Xylose in Escherichia coli BW25113

For carbon source D-Xylose across organisms

SEED Subsystems

Subsystem #Specific
Xylose utilization 4
Acetoin, butanediol metabolism 1
Branched-Chain Amino Acid Biosynthesis 1
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
D-xylose degradation I 2 2 2
xylitol degradation I 2 1 1
D-arabinitol degradation I 2 1 1
pyruvate fermentation to (R)-acetoin II 2 1 1
pyruvate fermentation to (S)-acetoin 3 3 1
pyruvate fermentation to (R)-acetoin I 3 2 1
L-valine biosynthesis 4 4 1
pyruvate fermentation to isobutanol (engineered) 5 4 1
superpathway of (R,R)-butanediol biosynthesis 5 2 1
thiazole component of thiamine diphosphate biosynthesis I 6 6 1
L-isoleucine biosynthesis IV 6 4 1
superpathway of 2,3-butanediol biosynthesis 6 3 1
L-isoleucine biosynthesis I (from threonine) 7 7 1
L-isoleucine biosynthesis III 7 4 1
L-isoleucine biosynthesis II 8 4 1
superpathway of branched chain amino acid biosynthesis 17 17 2
superpathway of glucose and xylose degradation 17 17 2
superpathway of thiamine diphosphate biosynthesis I 10 10 1
superpathway of L-isoleucine biosynthesis I 13 13 1
superpathway of L-threonine metabolism 18 16 1
superpathway of pentose and pentitol degradation 42 18 2