Experiment set1IT005 for Pseudomonas fluorescens GW456-L13

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D-Galactose carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + D-Galactose (20 mM), pH=7
Culturing: pseudo13_ML2, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 3/10/2015
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 10 genes in this experiment

For carbon source D-Galactose in Pseudomonas fluorescens GW456-L13

For carbon source D-Galactose across organisms

SEED Subsystems

Subsystem #Specific
D-galactonate catabolism 2
Alginate metabolism 1
D-Galacturonate and D-Glucuronate Utilization 1
Fatty Acid Biosynthesis FASII 1
mycolic acid synthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
D-galactonate degradation 3 3 2
D-galactose degradation II 2 1 1
gondoate biosynthesis (anaerobic) 4 4 1
octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) 12 9 3
palmitate biosynthesis III 29 21 7
tetradecanoate biosynthesis (mitochondria) 25 17 6
palmitate biosynthesis II (type II fatty acid synthase) 31 29 7
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) 9 9 2
oleate biosynthesis IV (anaerobic) 14 13 3
superpathway of fatty acids biosynthesis (E. coli) 53 51 11
superpathway of unsaturated fatty acids biosynthesis (E. coli) 20 20 4
cis-vaccenate biosynthesis 5 5 1
fatty acid elongation -- saturated 5 5 1
8-amino-7-oxononanoate biosynthesis IV 5 4 1
superpathway of fatty acid biosynthesis II (plant) 43 38 8
8-amino-7-oxononanoate biosynthesis I 11 9 2
odd iso-branched-chain fatty acid biosynthesis 34 24 6
anteiso-branched-chain fatty acid biosynthesis 34 24 6
even iso-branched-chain fatty acid biosynthesis 34 24 6
(5Z)-dodecenoate biosynthesis I 6 6 1
(5Z)-dodecenoate biosynthesis II 6 5 1
stearate biosynthesis II (bacteria and plants) 6 5 1
stearate biosynthesis IV 6 4 1
petroselinate biosynthesis 6 2 1
streptorubin B biosynthesis 34 20 5
L-glucose degradation 7 3 1
biotin biosynthesis I 15 13 2
superpathway of fatty acid biosynthesis I (E. coli) 16 15 2
2-allylmalonyl-CoA biosynthesis 8 2 1
mycolate biosynthesis 205 20 5
superpathway of mycolate biosynthesis 239 21 5