Experiment set1IT004 for Pseudomonas simiae WCS417

LB with Thallium(I) acetate 0.0625 mg/ml

Group: stress
Media: LB + Thallium(I) acetate (0.0625 mg/ml)
Culturing: fluoroDangl_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 2/19/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1140 D7,D8

Specific Phenotypes

For 11 genes in this experiment

For stress Thallium(I) acetate in Pseudomonas simiae WCS417

For stress Thallium(I) acetate across organisms

SEED Subsystems

Subsystem	#Specific
Choline and Betaine Uptake and Betaine Biosynthesis	5
Arsenic resistance	1
Bacterial Cell Division	1
Bacterial Cytoskeleton	1
Bacterial RNA-metabolizing Zn-dependent hydrolases	1
Biogenesis of cytochrome c oxidases	1
Glutathione-regulated potassium-efflux system and associated functions	1
Potassium homeostasis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glycine betaine biosynthesis I (Gram-negative bacteria)	2	2	2
choline degradation I	2	2	2
glycine betaine biosynthesis II (Gram-positive bacteria)	2	2	2
choline-O-sulfate degradation	3	3	2
β-alanine biosynthesis I	2	1	1
β-alanine biosynthesis IV	2	1	1
glycine betaine biosynthesis III (plants)	3	2	1
dimethylsulfoniopropanoate biosynthesis I (Wollastonia)	3	1	1
choline degradation IV	4	2	1
dimethylsulfoniopropanoate biosynthesis II (Spartina)	4	1	1