Experiment set1IT004 for Rhizobium sp. OAE497
D-Fructose carbon source
Group: carbon sourceMedia: RCH2_defined_noCarbon + D-Fructose (20 mM)
Culturing: Rhizobium_OAE497_ML5, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=700 rpm
By: Dennis/Robin on 4/4/22
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 5 genes in this experiment
For carbon source D-Fructose in Rhizobium sp. OAE497
For carbon source D-Fructose across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pentose phosphate pathway
- Fructose and mannose metabolism
- beta-Alanine metabolism
- Starch and sucrose metabolism
- Nucleotide sugars metabolism
- Pantothenate and CoA biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| ribose phosphorylation | 2 | 1 | 1 |
| D-sorbitol degradation I | 3 | 3 | 1 |
| 2-deoxy-D-ribose degradation I | 3 | 2 | 1 |
| sucrose degradation I (sucrose phosphotransferase) | 3 | 1 | 1 |
| phosphopantothenate biosynthesis I | 4 | 4 | 1 |
| sucrose degradation III (sucrose invertase) | 4 | 4 | 1 |
| sucrose degradation IV (sucrose phosphorylase) | 4 | 3 | 1 |
| sucrose degradation VII (sucrose 3-dehydrogenase) | 4 | 1 | 1 |
| mannitol cycle | 5 | 4 | 1 |
| sucrose degradation II (sucrose synthase) | 5 | 4 | 1 |
| superpathway of coenzyme A biosynthesis I (bacteria) | 9 | 8 | 1 |
| superpathway of coenzyme A biosynthesis II (plants) | 10 | 8 | 1 |
| heterolactic fermentation | 18 | 12 | 1 |
| superpathway of anaerobic sucrose degradation | 19 | 13 | 1 |