Experiment set1H40 for Kangiella aquimarina DSM 16071
marine broth with Doxycycline hyclate 0.03125 mg/ml
Group: stressMedia: marine_broth_2216 + Doxycycline hyclate (0.03125 mg/ml)
Culturing: Kang_ML4, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Jake on 9/24/2013
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 698 E7,E8
Specific Phenotypes
For 2 genes in this experiment
For stress Doxycycline hyclate in Kangiella aquimarina DSM 16071
For stress Doxycycline hyclate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Polyamine Metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| spermidine biosynthesis I | 2 | 2 | 1 |
| spermine biosynthesis | 2 | 1 | 1 |
| aminopropylcadaverine biosynthesis | 3 | 2 | 1 |
| spermidine biosynthesis III | 4 | 2 | 1 |
| superpathway of polyamine biosynthesis II | 8 | 5 | 1 |
| superpathway of polyamine biosynthesis I | 8 | 4 | 1 |
| L-methionine salvage cycle III | 11 | 4 | 1 |
| L-methionine salvage cycle I (bacteria and plants) | 12 | 3 | 1 |
| superpathway of arginine and polyamine biosynthesis | 17 | 12 | 1 |