Experiment set1H12 for Kangiella aquimarina DSM 16071
marine broth with Sodium nitrite 20 mM
Group: stressMedia: marine_broth_2216 + Sodium nitrite (20 mM)
Culturing: Kang_ML4, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Jake on 9/17/2013
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 692 A1,A2
Specific Phenotypes
For 9 genes in this experiment
For stress Sodium nitrite in Kangiella aquimarina DSM 16071
For stress Sodium nitrite across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Cysteine Biosynthesis | 1 |
| DNA-binding regulatory proteins, strays | 1 |
| Oxidative stress | 1 |
| Thioredoxin-disulfide reductase | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| siroheme biosynthesis | 4 | 4 | 2 |
| factor 430 biosynthesis | 7 | 3 | 2 |
| L-leucine degradation I | 6 | 4 | 1 |
| cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) | 13 | 2 | 2 |
| cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) | 15 | 3 | 2 |
| adenosylcobalamin biosynthesis II (aerobic) | 33 | 12 | 2 |
| adenosylcobalamin biosynthesis I (anaerobic) | 36 | 12 | 2 |