Experiment set19IT084 for Escherichia coli BW25113
P2 dilution 10-3 cacl2
Group: phageMedia: LB_plus_SM_buffer + P2_phage (0.001 MOI)
Culturing: Keio_ML9a, 48 well microplate; Tecan Infinite F200, Aerobic, at 37 (C), shaken=orbital
By: Vivek on 19-Jan-17
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 4 genes in this experiment
For phage P2_phage in Escherichia coli BW25113
For phage P2_phage across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| DNA-replication | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| heme b biosynthesis V (aerobic) | 4 | 4 | 1 |
| heme b biosynthesis I (aerobic) | 4 | 4 | 1 |
| heme b biosynthesis II (oxygen-independent) | 4 | 4 | 1 |
| superpathway of heme b biosynthesis from uroporphyrinogen-III | 6 | 6 | 1 |
| superpathway of heme b biosynthesis from glycine | 8 | 7 | 1 |
| superpathway of heme b biosynthesis from glutamate | 10 | 10 | 1 |
| lipid A-core biosynthesis (E. coli K-12) | 10 | 9 | 1 |
| superpathway of Kdo2-lipid A biosynthesis | 25 | 24 | 1 |