Experiment set19IT056 for Pseudomonas putida KT2440

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Sodium acetate carbon source

Group: carbon source
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + Sodium acetate (40 mM) + Ammonium chloride (10 mM)
Culturing: Putida_ML5_PNNL, 96 deep-well microplate; 1 mL volume, Aerobic, at 30 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 3 genes in this experiment

For carbon source Sodium acetate in Pseudomonas putida KT2440

For carbon source Sodium acetate across organisms

SEED Subsystems

Subsystem #Specific
Ketoisovalerate oxidoreductase 1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acetate formation from acetyl-CoA (succinate) 1 1 1
acetate and ATP formation from acetyl-CoA III 1 1 1
acetate conversion to acetyl-CoA 1 1 1
superpathway of acetate utilization and formation 3 3 1
pyruvate fermentation to acetate V 3 3 1
ethanol degradation IV 3 3 1
ethanol degradation II 3 3 1
ethanol degradation III 3 2 1
pyruvate fermentation to acetate VI 3 2 1
L-isoleucine biosynthesis V 3 2 1
chitin deacetylation 4 2 1
2-methylcitrate cycle I 5 5 1
β-alanine biosynthesis II 6 5 1
2-methylcitrate cycle II 6 5 1
L-isoleucine biosynthesis IV 6 4 1
superpathway of bitter acids biosynthesis 18 3 3
lupulone and humulone biosynthesis 6 1 1
adlupulone and adhumulone biosynthesis 6 1 1
colupulone and cohumulone biosynthesis 6 1 1
succinate fermentation to butanoate 7 1 1
TCA cycle VII (acetate-producers) 9 8 1
reductive glycine pathway of autotrophic CO2 fixation 9 5 1
cis-geranyl-CoA degradation 9 2 1
superpathway of coenzyme A biosynthesis II (plants) 10 9 1
anaerobic energy metabolism (invertebrates, mitochondrial) 10 5 1
superpathway of anaerobic energy metabolism (invertebrates) 17 10 1