Experiment set18IT039 for Pseudomonas putida KT2440

Growth at 25C

Group: temperature
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + D-Glucose (10 mM) + Ammonium chloride (10 mM)
Culturing: Putida_ML5_PNNL, 24-well transparent microplate; Multitron, Aerobic, at 25 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 5 genes in this experiment

For temperature D-Glucose in Pseudomonas putida KT2440

For temperature D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
Ammonia assimilation	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glutamate metabolism
• Nitrogen metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamate biosynthesis I	2	2	2
L-glutamine degradation I	1	1	1
L-glutamine degradation II	1	1	1
ammonia assimilation cycle III	3	3	2
L-glutamate and L-glutamine biosynthesis	7	6	2
L-asparagine biosynthesis III (tRNA-dependent)	4	4	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	3	1
L-citrulline biosynthesis	8	7	1
superpathway of L-citrulline metabolism	12	9	1