Experiment set18IT023 for Pseudomonas simiae WCS417

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Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic acid monopotassium salt monohydrate; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50

Group: motility_chemotaxis
Media: RCH2_defined_NO_salts_with_Glycerol + Method=Plug_approach; Chemical=L-Glutamic acid monopotassium salt monohydrate; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
Culturing: fluoroDangl_ML3, soft agar plate, Aerobic, at 30 (C), shaken=0 rpm, (solid)
By: Peter Kim on 17-Aug-21
Media components: 2.2 mM Glycerol, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 1 genes in this experiment

For motility_chemotaxis Method=Plug_approach; Chemical=L-Glutamic acid monopotassium salt monohydrate; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 in Pseudomonas simiae WCS417

For motility_chemotaxis Method=Plug_approach; Chemical=L-Glutamic acid monopotassium salt monohydrate; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 across organisms

SEED Subsystems

Subsystem #Specific
Arginine Deiminase Pathway 1
Arginine and Ornithine Degradation 1
Polyamine Metabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.