Experiment set18IT015 for Escherichia coli BW25113

Compare to:

LB with 3-methyl-3-butenol (commercial) 0.5 vol%

200 most detrimental genes:

  gene name fitness t score description  
b0462 acrB +1.5 13.9 multidrug efflux system protein (NCBI) compare
b4217 ytfK +1.3 7.1 orf, hypothetical protein (VIMSS) compare
b0564 appY +1.1 1.4 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b0463 acrA +1.1 7.6 multidrug efflux system (NCBI) compare
b2024 hisA +1.0 3.5 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b1778 yeaA +1.0 3.1 methionine sulfoxide reductase B (NCBI) compare
b1824 yobF +1.0 2.7 hypothetical protein (NCBI) compare
b2356 yfdM +1.0 2.8 orf, hypothetical protein (VIMSS) compare
b1038 csgF +1.0 3.5 predicted transport protein (NCBI) compare
b4396 rob +0.9 7.7 DNA-binding transcriptional activator (NCBI) compare
b4402 yjjY +0.9 1.6 hypothetical protein (NCBI) compare
b1074 flgC +0.9 3.0 flagellar basal-body rod protein C (NCBI) compare
b4406 yaeP +0.9 1.5 hypothetical protein (NCBI) compare
b3229 sspA +0.9 3.2 stringent starvation protein A (NCBI) compare
b1357 ydaS +0.9 2.6 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b3341 rpsG +0.9 1.6 30S ribosomal protein S7 (NCBI) compare
b2741 rpoS +0.8 5.2 RNA polymerase sigma factor (NCBI) compare
b1461 ydcE +0.8 1.2 4-oxalocrotonate tautomerase (NCBI) compare
b1095 fabF +0.8 5.9 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b1823 cspC +0.8 2.4 stress protein, member of the CspA-family (NCBI) compare
b2058 wcaB +0.8 1.5 predicted acyl transferase (NCBI) compare
b2642 yfjW +0.8 1.0 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b1295 ymjA +0.7 2.1 hypothetical protein (NCBI) compare
b3979 thrT +0.7 0.4 tRNA-Thr (NCBI) compare
b0998 torD +0.7 1.2 chaperone protein TorD (NCBI) compare
b4172 hfq +0.7 0.7 RNA-binding protein Hfq (NCBI) compare
b4431 rprA +0.7 1.6 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b2258 b2258 +0.7 1.3 putative transport/receptor protein (VIMSS) compare
b1493 gadB +0.7 0.8 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b1753 ynjA +0.7 2.4 hypothetical protein (NCBI) compare
b1591 b1591 +0.7 1.0 putative oxidoreductase component (VIMSS) compare
b0605 ahpC +0.7 2.3 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b2814 metZ +0.6 1.3 tRNA-Met (NCBI) compare
b1765 ydjA +0.6 0.7 predicted oxidoreductase (NCBI) compare
b4441 glmY +0.6 1.8 sRNA activator of glmS mRNA (RefSeq) compare
b2009 sbmC +0.6 1.6 DNA gyrase inhibitor (NCBI) compare
b3977 tyrU +0.6 0.8 tRNA-Tyr (NCBI) compare
b1806 yeaY +0.6 2.3 predicted lipoprotein (NCBI) compare
b4071 nrfB +0.6 2.3 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b0990 cspG +0.6 0.7 DNA-binding transcriptional regulator (NCBI) compare
b4647 mokA +0.6 0.8 no description compare
b3888 yiiD +0.6 4.4 predicted acetyltransferase (NCBI) compare
b1305 pspB +0.6 1.6 phage shock protein B (NCBI) compare
b0746 valZ +0.6 1.5 tRNA-Val (NCBI) compare
b1954 dsrA +0.6 2.1 regulatory antisense RNA (NCBI) compare
b1233 ychJ +0.6 3.1 hypothetical protein (NCBI) compare
b2673 nrdH +0.6 2.0 glutaredoxin-like protein (NCBI) compare
b1335 ogt +0.6 1.8 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare
b2493 yfgO +0.6 3.6 predicted inner membrane protein (NCBI) compare
b1685 ydiH +0.6 1.0 predicted protein (RefSeq) compare
b4630 ykgP +0.6 1.3 no description compare
b1582 ynfA +0.5 1.9 hypothetical protein (NCBI) compare
b3129 sohA +0.5 2.6 predicted regulator (NCBI) compare
b1792 yeaO +0.5 2.5 orf, hypothetical protein (VIMSS) compare
b4503 yafF +0.5 0.7 no description compare
b1743 spy +0.5 3.1 envelope stress induced periplasmic protein (NCBI) compare
b1326 mpaA +0.5 2.5 murein peptide amidase A (RefSeq) compare
b1414 ydcF +0.5 2.9 hypothetical protein (NCBI) compare
b2168 fruK +0.5 0.7 1-phosphofructokinase (NCBI) compare
b4592 yccB +0.5 1.3 hypothetical protein (RefSeq) compare
b1919 yedO +0.5 2.2 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b1394 paaG +0.5 2.2 enoyl-CoA hydratase (NCBI) compare
b1333 uspE +0.5 1.7 stress-induced protein (NCBI) compare
b0881 clpS +0.5 0.6 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b4548 ypjJ +0.5 2.2 hypothetical protein (NCBI) compare
b4557 yidD +0.5 1.4 hypothetical protein (NCBI) compare
b2490 b2490 +0.5 2.0 putative protein processing element (VIMSS) compare
b3658 selC +0.5 1.0 tRNA-Sec (NCBI) compare
b2446 yffO +0.5 1.6 CPZ-55 prophage; predicted protein (NCBI) compare
b4215 ytfI +0.5 1.0 hypothetical protein (NCBI) compare
b4523 yciX +0.5 2.3 hypothetical protein (RefSeq) compare
b3858 yihD +0.5 2.1 hypothetical protein (NCBI) compare
b1831 proQ +0.5 2.1 putative solute/DNA competence effector (NCBI) compare
b3163 nlpI +0.5 2.4 hypothetical protein (NCBI) compare
b1086 rluC +0.5 2.7 23S rRNA pseudouridylate synthase (NCBI) compare
b0456 ybaA +0.5 1.8 hypothetical protein (NCBI) compare
b1566 flxA +0.5 1.3 Qin prophage; predicted protein (NCBI) compare
b2262 menB +0.5 1.7 naphthoate synthase (NCBI) compare
b1659 ydhB +0.5 1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b4048 yjbM +0.5 0.9 hypothetical protein (NCBI) compare
b2637 yfjT +0.5 1.4 CP4-57 prophage; predicted protein (NCBI) compare
b1661 cfa +0.5 2.1 cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI) compare
b4622 ytcA +0.5 2.0 hypothetical protein (RefSeq) compare
b0218 yafU +0.5 1.5 predicted inner membrane protein (RefSeq) compare
b0655 gltI +0.5 1.5 glutamate and aspartate transporter subunit (NCBI) compare
b2096 gatY +0.5 1.5 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b1647 ydhF +0.4 2.2 predicted oxidoreductase (NCBI) compare
b2659 b2659 +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b1279 yciS +0.4 0.6 conserved inner membrane protein (NCBI) compare
b1518 lsrG +0.4 0.7 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b1961 dcm +0.4 1.1 DNA cytosine methylase (NCBI) compare
b1639 ydhA +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b1298 ycjL +0.4 2.2 probable amidotransferase subunit (VIMSS) compare
b1848 yebG +0.4 1.0 conserved protein regulated by LexA (NCBI) compare
b3355 prkB +0.4 3.7 predicted phosphoribulokinase (NCBI) compare
b0965 b0965 +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b2579 yfiD +0.4 1.4 pyruvate formate lyase subunit (NCBI) compare
b1329 b1329 +0.4 2.8 putative transport periplasmic protein (VIMSS) compare
b1963 b1963 +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b1266 yciV +0.4 1.3 hypothetical protein (NCBI) compare
b1708 nlpC +0.4 1.7 predicted lipoprotein (NCBI) compare
b3155 yhbQ +0.4 1.5 hypothetical protein (NCBI) compare
b4704 arrS +0.4 0.2 Antisense sRNA ArrS, function unknown (NCBI) compare
b0018 mokC +0.4 0.8 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b1355 b1355 +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b1473 yddG +0.4 2.3 predicted methyl viologen efflux pump (RefSeq) compare
b2535 csiE +0.4 3.3 orf, hypothetical protein (VIMSS) compare
b1514 lsrC +0.4 1.2 AI2 transporter (NCBI) compare
b1334 fnr +0.4 1.4 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b4651 yibW +0.4 0.2 no description compare
b2377 yfdY +0.4 1.0 predicted inner membrane protein (NCBI) compare
b1751 ydjY +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b4453 ldrD +0.4 1.1 toxic polypeptide, small (NCBI) compare
b1108 ycfP +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b4679 yohP +0.4 1.4 expressed protein (RefSeq) compare
b1148 ymfM +0.4 1.3 e14 prophage; predicted protein (NCBI) compare
b4104 phnE +0.4 1.5 phosphonate/organophosphate ester transporter subunit (NCBI) compare
b1250 kch +0.4 2.3 voltage-gated potassium channel (NCBI) compare
b0653 gltK +0.4 2.8 glutamate and aspartate transporter subunit (NCBI) compare
b0542 renD +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b1686 ydiI +0.4 1.1 hypothetical protein (NCBI) compare
b0398 sbcD +0.4 1.9 exonuclease, dsDNA, ATP-dependent (NCBI) compare
b1399 paaX +0.4 1.3 DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI) compare
b2136 yohD +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b3483 yhhH +0.4 0.2 orf, hypothetical protein (VIMSS) compare
b4282 yjhE +0.4 2.0 orf, hypothetical protein (VIMSS) compare
b4397 creA +0.4 1.4 hypothetical protein (NCBI) compare
b2181 yejG +0.4 1.4 hypothetical protein (NCBI) compare
b3636 rpmG +0.4 1.0 50S ribosomal protein L33 (NCBI) compare
b2571 rseB +0.4 2.3 periplasmic negative regulator of sigmaE (NCBI) compare
b1013 ycdC +0.4 1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b1221 narL +0.4 1.9 DNA-binding response regulator in two-component regulatory system with NarX (or NarQ) (NCBI) compare
b1341 b1341 +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b3443 yrhA +0.4 0.6 orf, hypothetical protein (VIMSS) compare
b2783 chpR +0.4 1.4 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b3395 yrfD +0.4 2.9 orf, hypothetical protein (VIMSS) compare
b2390 ypeC +0.4 1.3 hypothetical protein (NCBI) compare
b0870 ltaE +0.4 2.3 L-allo-threonine aldolase, PLP-dependent (NCBI) compare
b4480 hdfR +0.4 1.8 transcriptional regulator HdfR (NCBI) compare
b2335 yfcR +0.4 2.0 predicted fimbrial-like adhesin protein (NCBI) compare
b1299 puuR +0.4 1.5 DNA-binding transcriptional repressor (NCBI) compare
b4538 yoeF +0.4 0.6 hypothetical protein (RefSeq) compare
b1492 gadC +0.4 2.1 predicted glutamate:gamma-aminobutyric acid antiporter (NCBI) compare
b4371 rsmC +0.3 2.7 16S ribosomal RNA m2G1207 methyltransferase (NCBI) compare
b4701 sokX +0.3 0.3 sok-related sRNA, function unknown (RefSeq) compare
b1143 ymfI +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b4533 ynfO +0.3 0.5 Qin prophage; predicted protein (NCBI) compare
b0505 allA +0.3 1.7 ureidoglycolate hydrolase (NCBI) compare
b2069 yegD +0.3 2.5 putative heat shock protein (VIMSS) compare
b1922 fliA +0.3 2.2 flagellar biosynthesis; alternative sigma factor 28; regulation of flagellar operons (VIMSS) compare
b3199 yrbK +0.3 1.0 hypothetical protein (NCBI) compare
b1085 b1085 +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b1380 ldhA +0.3 0.9 D-lactate dehydrogenase (NCBI) compare
b0810 glnP +0.3 1.3 glutamine ABC transporter permease protein (NCBI) compare
b1336 ydaH +0.3 1.7 putative pump protein (transport) (VIMSS) compare
b1308 pspE +0.3 1.7 thiosulfate:cyanide sulfurtransferase (rhodanese) (NCBI) compare
b0240 crl +0.3 2.3 DNA-binding transcriptional regulator (NCBI) compare
b2195 ccmG +0.3 1.6 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b2823 ppdC +0.3 2.1 hypothetical protein (NCBI) compare
b2250 b2250 +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b4532 yncN +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b1643 ydhI +0.3 2.0 predicted inner membrane protein (NCBI) compare
b1431 ydcL +0.3 1.2 predicted lipoprotein (NCBI) compare
b2093 gatB +0.3 1.1 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b1531 marA +0.3 1.2 multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS) compare
b2704 srlB +0.3 1.3 glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI) compare
b1070 flgN +0.3 0.6 export chaperone for FlgK and FlgL (NCBI) compare
b1246 oppD +0.3 2.2 oligopeptide transporter ATP-binding component (NCBI) compare
b3912 cpxR +0.3 2.2 DNA-binding response regulator in two-component regulatory system with CpxA (NCBI) compare
b4299 yjhI +0.3 1.9 KpLE2 phage-like element; predicted DNA-binding transcriptional regulator (NCBI) compare
b0785 moaE +0.3 1.1 molybdopterin synthase, large subunit (NCBI) compare
b3725 pstB +0.3 0.2 phosphate transporter subunit (NCBI) compare
b2433 b2433 +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b1219 ychN +0.3 1.1 hypothetical protein (NCBI) compare
b3257 yhdT +0.3 1.8 conserved inner membrane protein (NCBI) compare
b4582 yoeA +0.3 1.0 no description compare
b0798 ybiA +0.3 1.0 hypothetical protein (NCBI) compare
b1096 pabC +0.3 1.8 4-amino-4-deoxychorismate lyase (NCBI) compare
b2300 yfcE +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b1942 fliJ +0.3 0.5 flagellar biosynthesis chaperone (NCBI) compare
b3243 aaeR +0.3 1.2 predicted DNA-binding transcriptional regulator, efflux system (NCBI) compare
b2186 yejK +0.3 2.6 nucleoid-associated protein NdpA (NCBI) compare
b1053 mdtG +0.3 1.8 predicted drug efflux system (NCBI) compare
b2948 yqgE +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b2541 hcaB +0.3 1.5 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase (NCBI) compare
b1512 lsrR +0.3 1.5 lsr operon transcriptional repressor (NCBI) compare
b2376 ypdI +0.3 1.0 predicted lipoprotein involved in colanic acid biosynthesis (NCBI) compare
b1771 ydjG +0.3 1.5 predicted oxidoreductase (NCBI) compare
b1698 ydiR +0.3 1.1 predicted electron transfer flavoprotein, FAD-binding (NCBI) compare
b1619 hdhA +0.3 1.9 7-alpha-hydroxysteroid dehydrogenase (NCBI) compare
b0388 aroL +0.3 1.8 shikimate kinase II (NCBI) compare
b1285 gmr +0.3 1.7 modulator of Rnase II stability (NCBI) compare
b3929 rraA +0.3 2.1 ribonuclease activity regulator protein RraA (NCBI) compare
b1060 yceP +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b2765 ygcM +0.3 1.2 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) (NCBI) compare
b4702 mgtL +0.3 0.7 regulatory leader peptide for mgtA (NCBI) compare
b1184 umuC +0.3 2.2 DNA polymerase V subunit UmuC (NCBI) compare
b3504 yhiS +0.3 0.1 hypothetical protein (RefSeq) compare
b0782 moaB +0.3 0.8 molybdopterin biosynthesis protein B (NCBI) compare
b2736 ygbJ +0.3 1.2 predicted dehydrogenase, with NAD(P)-binding Rossmann-fold domain (NCBI) compare


Specific Phenotypes

For 2 genes in this experiment

For stress 3-methyl-3-butenol (commercial) in Escherichia coli BW25113

For stress 3-methyl-3-butenol (commercial) across organisms