Experiment set17IT064 for Escherichia coli BW25113

Compare to:

LB with Cholinium hydrogen phosphate 10 vol%

200 most detrimental genes:

  gene name fitness t score description  
b1830 prc +2.3 13.3 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b0033 carB +1.6 14.3 carbamoyl-phosphate synthase large subunit (NCBI) compare
b4313 fimE +1.5 9.1 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b4503 yafF +1.4 1.0 no description compare
b3443 yrhA +1.4 1.4 orf, hypothetical protein (VIMSS) compare
b3979 thrT +1.4 0.7 tRNA-Thr (NCBI) compare
b1984 asnW +1.4 2.1 tRNA-Asn (NCBI) compare
b2741 rpoS +1.4 9.0 RNA polymerase sigma factor (NCBI) compare
b0032 carA +1.3 6.5 carbamoyl-phosphate synthase small subunit (NCBI) compare
b2110 yehC +1.2 2.0 predicted periplasmic pilin chaperone (NCBI) compare
b2282 nuoH +1.2 4.0 NADH dehydrogenase subunit H (NCBI) compare
b3163 nlpI +1.1 4.3 hypothetical protein (NCBI) compare
b1362 rzpR +1.1 2.8 putative Rac prophage endopeptidase (VIMSS) compare
b1346 ydaQ +1.1 1.4 orf, hypothetical protein (VIMSS) compare
b1070 flgN +1.1 2.0 export chaperone for FlgK and FlgL (NCBI) compare
b1557 cspB +1.1 2.7 Qin prophage; cold shock protein (NCBI) compare
b1524 yneH +1.1 1.3 predicted glutaminase (NCBI) compare
b2287 nuoB +1.1 4.5 NADH dehydrogenase subunit B (NCBI) compare
b2284 nuoF +1.1 5.3 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b1865 nudB +1.1 1.6 dATP pyrophosphohydrolase (NCBI) compare
b1942 fliJ +1.0 1.2 flagellar biosynthesis chaperone (NCBI) compare
b2111 yehD +1.0 3.5 predicted fimbrial-like adhesin protein (NCBI) compare
b1108 ycfP +1.0 2.0 orf, hypothetical protein (VIMSS) compare
b3250 mreC +0.9 1.3 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b2531 iscR +0.9 2.0 DNA-binding transcriptional repressor (NCBI) compare
b2279 nuoK +0.9 1.9 NADH dehydrogenase subunit K (NCBI) compare
b1685 ydiH +0.9 1.6 predicted protein (RefSeq) compare
b2283 nuoG +0.9 5.8 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b4402 yjjY +0.9 1.0 hypothetical protein (NCBI) compare
b2939 yqgB +0.9 1.5 predicted protein (RefSeq) compare
b3199 yrbK +0.9 1.8 hypothetical protein (NCBI) compare
b1831 proQ +0.9 2.9 putative solute/DNA competence effector (NCBI) compare
b0934 ycbM +0.9 1.6 putative transport system permease protein (VIMSS) compare
b4592 yccB +0.8 2.1 hypothetical protein (RefSeq) compare
b2276 nuoN +0.8 4.3 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b1357 ydaS +0.8 1.4 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b4647 mokA +0.8 0.7 no description compare
b3405 ompR +0.8 3.9 osmolarity response regulator (NCBI) compare
b2277 nuoM +0.8 4.9 NADH dehydrogenase subunit M (NCBI) compare
b2516 yfgA +0.8 4.0 hypothetical protein (NCBI) compare
b3672 ivbL +0.8 2.1 ilvB operon leader peptide (NCBI) compare
b0724 sdhB +0.8 3.8 succinate dehydrogenase, FeS subunit (NCBI) compare
b2911 ssrS +0.8 1.7 6S regulatory RNA (NCBI) compare
b2281 nuoI +0.8 3.3 NADH dehydrogenase subunit I (NCBI) compare
b4699 fnrS +0.8 0.6 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b2642 yfjW +0.8 0.7 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b3620 rfaF +0.8 3.9 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b1259 yciG +0.8 1.4 orf, hypothetical protein (VIMSS) compare
b3935 priA +0.8 1.9 primosome assembly protein PriA (NCBI) compare
b3889 yiiE +0.7 2.1 predicted transcriptional regulator (RefSeq) compare
b2280 nuoJ +0.7 3.4 NADH dehydrogenase subunit J (NCBI) compare
b0142 folK +0.7 0.4 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b2278 nuoL +0.7 5.2 NADH dehydrogenase subunit L (NCBI) compare
b1823 cspC +0.7 1.1 stress protein, member of the CspA-family (NCBI) compare
b4201 priB +0.7 1.4 primosomal replication protein N (NCBI) compare
b4631 ykgQ +0.7 1.7 no description compare
b0721 sdhC +0.7 3.0 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b1862 yebB +0.7 2.4 orf, hypothetical protein (VIMSS) compare
b2897 ygfY +0.7 3.7 hypothetical protein (NCBI) compare
b0049 apaH +0.7 3.1 diadenosinetetraphosphatase (NCBI) compare
b0564 appY +0.7 0.8 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b2082 ogrK +0.7 1.6 DNA-binding transcriptional regulator prophage P2 remnant (NCBI) compare
b0722 sdhD +0.7 2.4 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b0014 dnaK +0.7 0.9 molecular chaperone DnaK (NCBI) compare
b1591 b1591 +0.7 1.7 putative oxidoreductase component (VIMSS) compare
b3619 rfaD +0.7 2.8 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b4406 yaeP +0.7 2.5 hypothetical protein (NCBI) compare
b4694 yagP +0.7 2.5 no description compare
b0125 hpt +0.7 2.9 hypoxanthine phosphoribosyltransferase (VIMSS) compare
b2477 nlpB +0.7 2.2 lipoprotein-34 (VIMSS) compare
b4520 ymgF +0.7 1.0 hypothetical protein (NCBI) compare
b0723 sdhA +0.6 4.8 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b4493 gapC +0.6 1.5 no description compare
b3621 rfaC +0.6 3.4 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b4527 b1354 +0.6 2.3 orf, hypothetical protein (VIMSS) compare
b1724 ydiZ +0.6 2.0 hypothetical protein (NCBI) compare
b0222 lpcA +0.6 1.7 phosphoheptose isomerase (NCBI) compare
b2285 nuoE +0.6 2.2 NADH dehydrogenase subunit E (NCBI) compare
b1251 yciI +0.6 2.5 orf, hypothetical protein (VIMSS) compare
b2446 yffO +0.6 2.0 CPZ-55 prophage; predicted protein (NCBI) compare
b1446 ydcY +0.6 1.3 hypothetical protein (NCBI) compare
b2829 ptsP +0.6 4.9 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b1279 yciS +0.6 0.8 conserved inner membrane protein (NCBI) compare
b1151 ymfO +0.6 1.9 e14 prophage; conserved protein (RefSeq) compare
b2738 ygbL +0.6 2.6 hypothetical protein (NCBI) compare
b3649 rpoZ +0.6 1.7 DNA-directed RNA polymerase subunit omega (NCBI) compare
b4127 yjdJ +0.6 3.6 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b4397 creA +0.6 2.6 hypothetical protein (NCBI) compare
b4071 nrfB +0.6 1.7 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b4451 ryhB +0.6 1.5 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b2085 b2085 +0.6 2.2 orf, hypothetical protein (VIMSS) compare
b1607 ydgC +0.6 1.1 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b4431 rprA +0.6 1.4 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b1778 yeaA +0.6 1.8 methionine sulfoxide reductase B (NCBI) compare
b1400 paaY +0.6 1.5 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b2346 vacJ +0.6 3.8 predicted lipoprotein (NCBI) compare
b3764 yifE +0.5 3.2 hypothetical protein (NCBI) compare
b1686 ydiI +0.5 1.1 hypothetical protein (NCBI) compare
b2309 hisJ +0.5 1.9 histidine/lysine/arginine/ornithine transporter subunit (NCBI) compare
b2286 nuoC +0.5 2.8 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b3622 rfaL +0.5 2.0 O-antigen ligase (NCBI) compare
b4363 yjjB +0.5 1.7 conserved inner membrane protein (RefSeq) compare
b4172 hfq +0.5 0.7 RNA-binding protein Hfq (NCBI) compare
b4533 ynfO +0.5 1.6 Qin prophage; predicted protein (NCBI) compare
b0604 dsbG +0.5 3.6 thiol:disulfide interchange protein (VIMSS) compare
b4505 insX +0.5 1.2 predicted IS protein (RefSeq) compare
b0217 yafT +0.5 2.5 predicted aminopeptidase (NCBI) compare
b2236 yfaE +0.5 1.6 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b3761 trpT +0.5 1.1 tRNA-Trp (NCBI) compare
b4506 ykgO +0.5 2.5 rpmJ (L36) paralog (NCBI) compare
b0143 pcnB +0.5 2.8 poly(A) polymerase I (VIMSS) compare
b3261 fis +0.5 1.3 DNA-binding protein Fis (NCBI) compare
b4552 yrhC +0.5 0.6 no description compare
b0784 moaD +0.5 0.7 molybdopterin synthase, small subunit (NCBI) compare
b3183 obgE +0.5 0.6 GTPase involved in cell partioning and DNA repair (NCBI) compare
b4679 yohP +0.5 1.2 expressed protein (RefSeq) compare
b1566 flxA +0.5 1.7 Qin prophage; predicted protein (NCBI) compare
b2948 yqgE +0.5 1.8 orf, hypothetical protein (VIMSS) compare
b3890 yiiF +0.5 1.4 orf, hypothetical protein (VIMSS) compare
b2501 ppk +0.5 3.5 polyphosphate kinase (NCBI) compare
b3181 greA +0.5 2.1 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1085 b1085 +0.5 1.4 orf, hypothetical protein (VIMSS) compare
b1742 b1742 +0.5 1.9 orf, hypothetical protein (VIMSS) compare
b1177 ycgJ +0.5 3.1 hypothetical protein (NCBI) compare
b1195 ymgE +0.5 1.4 predicted inner membrane protein (NCBI) compare
b3781 trxA +0.5 1.9 thioredoxin 1 (VIMSS) compare
b4633 xisD +0.5 2.3 no description compare
b4515 ybgT +0.5 1.1 hypothetical protein (NCBI) compare
b3643 rph +0.5 2.6 ribonuclease PH (NCBI) compare
b1647 ydhF +0.5 1.3 predicted oxidoreductase (NCBI) compare
b3150 yraP +0.5 2.9 hypothetical protein (NCBI) compare
b3052 rfaE +0.5 2.3 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b0238 gpt +0.5 1.2 xanthine phosphoribosyltransferase (NCBI) compare
b4587 insN +0.5 3.1 no description compare
b4649 ysaD +0.5 2.2 no description compare
b4360 yjjA +0.5 2.2 putative glycoprotein/receptor (VIMSS) compare
b2742 nlpD +0.5 2.8 predicted outer membrane lipoprotein (NCBI) compare
b1150 ymfR +0.5 0.6 e14 prophage; predicted protein (NCBI) compare
b1573 ydfC +0.5 0.3 Qin prophage; predicted protein (RefSeq) compare
b1384 feaR +0.5 2.2 DNA-binding transcriptional dual regulator (NCBI) compare
b3467 yhhM +0.5 2.0 hypothetical protein (NCBI) compare
b2659 b2659 +0.5 1.6 orf, hypothetical protein (VIMSS) compare
b4570 lomR +0.5 0.7 no description compare
b2199 ccmC +0.5 1.6 heme exporter subunit (NCBI) compare
b1572 ydfB +0.5 0.9 orf, hypothetical protein (VIMSS) compare
b1842 holE +0.4 1.3 DNA polymerase III, theta subunit (NCBI) compare
b0583 entD +0.4 1.8 phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex (NCBI) compare
b1044 ymdA +0.4 2.0 hypothetical protein (NCBI) compare
b1360 ydaV +0.4 0.6 Rac prophage; predicted DNA replication protein (NCBI) compare
b1139 lit +0.4 2.1 e14 prophage; cell death peptidase, inhibitor of T4 late gene expression (NCBI) compare
b3341 rpsG +0.4 0.4 30S ribosomal protein S7 (NCBI) compare
b1183 umuD +0.4 1.1 DNA polymerase V, subunit D (NCBI) compare
b0751 pnuC +0.4 2.2 predicted nicotinamide mononucleotide transporter (NCBI) compare
b4648 ysaC +0.4 1.8 no description compare
b3102 yqjG +0.4 1.9 predicted S-transferase (NCBI) compare
b1155 ymfS +0.4 1.6 e14 prophage; predicted protein (NCBI) compare
b0380 b0380 +0.4 2.6 orf, hypothetical protein (VIMSS) compare
b2512 yfgL +0.4 2.3 protein assembly complex, lipoprotein component (NCBI) compare
b1708 nlpC +0.4 1.9 predicted lipoprotein (NCBI) compare
b3785 wzzE +0.4 3.3 putative transport protein (VIMSS) compare
b2638 yfjU +0.4 1.3 CP4-57 prophage; conserved protein (RefSeq) compare
b3042 b3042 +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b1527 yneK +0.4 1.2 hypothetical protein (NCBI) compare
b2564 pdxJ +0.4 1.4 pyridoxal phosphate biosynthetic protein (NCBI) compare
b1075 flgD +0.4 1.3 flagellar basal body rod modification protein D (NCBI) compare
b4571 wbbL +0.4 1.6 no description compare
b4702 mgtL +0.4 1.1 regulatory leader peptide for mgtA (NCBI) compare
b3021 ygiT +0.4 1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b1839 yebY +0.4 1.5 hypothetical protein (NCBI) compare
b1612 fumA +0.4 2.1 fumarate hydratase (fumarase A), aerobic Class I (NCBI) compare
b3867 hemN +0.4 3.3 O2-independent coproporphyrinogen III oxidase (VIMSS) compare
b1298 ycjL +0.4 2.1 probable amidotransferase subunit (VIMSS) compare
b2876 b2876 +0.4 2.6 orf, hypothetical protein (VIMSS) compare
b1828 b1828 +0.4 2.4 putative transport protein (VIMSS) compare
b4293 fecI +0.4 1.6 KpLE2 phage-like element; RNA polymerase, sigma 19 factor (NCBI) compare
b2096 gatY +0.4 1.6 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b4589 ylcI +0.4 0.7 hypothetical protein (RefSeq) compare
b1433 b1433 +0.4 2.3 putative membrane transport protein (VIMSS) compare
b1804 rnd +0.4 2.0 ribonuclease D (NCBI) compare
b1190 dadX +0.4 2.1 alanine racemase (NCBI) compare
b3716 yieI +0.4 2.7 predicted inner membrane protein (NCBI) compare
b0364 yaiS +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b3753 rbsR +0.4 3.0 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b2299 yfcD +0.4 1.4 predicted NUDIX hydrolase (NCBI) compare
b3437 gntK +0.4 1.7 gluconokinase 2, thermoresistant (VIMSS) compare
b0502 ylbG +0.4 0.7 no description compare
b3875 ompL +0.4 1.1 predicted outer membrane porin L (NCBI) compare
b3206 npr +0.4 1.7 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b0726 sucA +0.4 1.8 alpha-ketoglutarate decarboxylase (NCBI) compare
b4608 ryfC +0.4 1.4 Novel sRNA, function unknown (RefSeq) compare
b1583 ynfB +0.4 1.6 hypothetical protein (NCBI) compare
b0379 yaiY +0.4 1.5 predicted inner membrane protein (NCBI) compare
b2372 yfdV +0.4 2.1 predicted transporter (NCBI) compare
b1943 fliK +0.4 1.9 flagellar hook-length control protein (NCBI) compare
b2960 yggH +0.4 2.5 tRNA(m7G46)-methyltransferase (NCBI) compare
b0278 yagL +0.4 2.5 CP4-6 prophage; DNA-binding protein (NCBI) compare
b3404 envZ +0.4 2.9 osmolarity sensor protein (NCBI) compare
b2808 gcvA +0.4 1.7 DNA-binding transcriptional dual regulator (NCBI) compare
b3811 xerC +0.4 0.6 site-specific tyrosine recombinase XerC (NCBI) compare
b4415 hokE +0.4 1.6 toxic polypeptide, small (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Cholinium hydrogen phosphate across organisms