Experiment set17IT063 for Escherichia coli BW25113

Compare to:

LB with Cholinium glutamate 10 vol%

200 most detrimental genes:

  gene name fitness t score description  
b4313 fimE +1.7 11.5 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b1830 prc +1.7 9.4 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b1192 ldcA +1.5 8.7 L,D-carboxypeptidase A (NCBI) compare
b1361 ydaW +1.3 0.8 orf, hypothetical protein (VIMSS) compare
b2531 iscR +1.2 2.3 DNA-binding transcriptional repressor (NCBI) compare
b3236 mdh +1.2 9.9 malate dehydrogenase (NCBI) compare
b1936 intG +1.2 1.7 predicted defective phage integrase (pseudogene) (RefSeq) compare
b4570 lomR +1.2 1.8 no description compare
b1524 yneH +1.1 1.6 predicted glutaminase (NCBI) compare
b0740 tolB +1.1 5.7 translocation protein TolB precursor (NCBI) compare
b1332 ynaJ +1.0 0.9 predicted inner membrane protein (NCBI) compare
b0033 carB +1.0 9.2 carbamoyl-phosphate synthase large subunit (NCBI) compare
b1058 yceO +1.0 0.5 hypothetical protein (NCBI) compare
b2829 ptsP +1.0 8.0 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b2830 nudH +1.0 1.6 dinucleoside polyphosphate hydrolase (NCBI) compare
b3261 fis +1.0 2.8 DNA-binding protein Fis (NCBI) compare
b2284 nuoF +1.0 5.3 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b3500 gor +1.0 1.6 glutathione reductase (NCBI) compare
b2741 rpoS +0.9 4.5 RNA polymerase sigma factor (NCBI) compare
b0723 sdhA +0.9 7.5 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b1205 ychH +0.9 3.4 predicted inner membrane protein (NCBI) compare
b2285 nuoE +0.9 3.0 NADH dehydrogenase subunit E (NCBI) compare
b2897 ygfY +0.9 5.3 hypothetical protein (NCBI) compare
b2282 nuoH +0.9 3.7 NADH dehydrogenase subunit H (NCBI) compare
b3387 dam +0.9 7.7 DNA adenine methylase (NCBI) compare
b3163 nlpI +0.9 4.5 hypothetical protein (NCBI) compare
b3026 qseC +0.9 4.2 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b1727 yniC +0.9 3.3 predicted hydrolase (NCBI) compare
b3199 yrbK +0.9 2.1 hypothetical protein (NCBI) compare
b3627 rfaI +0.9 6.8 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b0722 sdhD +0.9 3.5 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b2287 nuoB +0.9 3.5 NADH dehydrogenase subunit B (NCBI) compare
b2904 gcvH +0.8 3.6 glycine cleavage system protein H (NCBI) compare
b1362 rzpR +0.8 2.0 putative Rac prophage endopeptidase (VIMSS) compare
b1607 ydgC +0.8 1.9 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b0739 tolA +0.8 3.3 cell envelope integrity inner membrane protein TolA (NCBI) compare
b1984 asnW +0.8 1.3 tRNA-Asn (NCBI) compare
b1831 proQ +0.8 4.1 putative solute/DNA competence effector (NCBI) compare
b0720 gltA +0.8 5.0 citrate synthase (NCBI) compare
b0721 sdhC +0.8 3.4 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b0724 sdhB +0.8 4.3 succinate dehydrogenase, FeS subunit (NCBI) compare
b1476 fdnI +0.8 1.1 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b0738 tolR +0.8 2.8 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0049 apaH +0.8 2.9 diadenosinetetraphosphatase (NCBI) compare
b2283 nuoG +0.8 5.1 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b2288 nuoA +0.8 2.6 NADH dehydrogenase I chain A (VIMSS) compare
b1685 ydiH +0.8 1.5 predicted protein (RefSeq) compare
b0032 carA +0.8 4.6 carbamoyl-phosphate synthase small subunit (NCBI) compare
b3628 rfaB +0.8 6.0 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b2276 nuoN +0.8 3.6 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b0741 pal +0.7 2.9 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b3405 ompR +0.7 3.6 osmolarity response regulator (NCBI) compare
b4413 sokC +0.7 1.7 regulatory antisense RNA (NCBI) compare
b2278 nuoL +0.7 5.0 NADH dehydrogenase subunit L (NCBI) compare
b1954 dsrA +0.7 2.5 regulatory antisense RNA (NCBI) compare
b1418 cybB +0.7 2.4 cytochrome b(561) (VIMSS) compare
b2516 yfgA +0.7 3.9 hypothetical protein (NCBI) compare
b2281 nuoI +0.7 2.9 NADH dehydrogenase subunit I (NCBI) compare
b1357 ydaS +0.7 1.5 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b0737 tolQ +0.7 2.2 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b2939 yqgB +0.7 1.7 predicted protein (RefSeq) compare
b1085 b1085 +0.7 1.6 orf, hypothetical protein (VIMSS) compare
b2259 pmrD +0.7 1.1 polymyxin resistance protein B (VIMSS) compare
b4595 yciY +0.7 2.3 hypothetical protein (RefSeq) compare
b3192 yrbC +0.7 5.6 predicted ABC-type organic solvent transporter (NCBI) compare
b4397 creA +0.7 3.5 hypothetical protein (NCBI) compare
b3195 yrbF +0.7 5.2 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b1591 b1591 +0.7 1.6 putative oxidoreductase component (VIMSS) compare
b0580 ybdJ +0.7 1.7 predicted inner membrane protein (NCBI) compare
b2356 yfdM +0.6 2.0 orf, hypothetical protein (VIMSS) compare
b1814 sdaA +0.6 2.2 L-serine deaminase I (NCBI) compare
b1448 yncA +0.6 0.9 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b4631 ykgQ +0.6 1.8 no description compare
b1777 b1777 +0.6 2.8 orf, hypothetical protein (VIMSS) compare
b4687 shoB +0.6 3.9 toxic membrane protein (RefSeq) compare
b2346 vacJ +0.6 4.1 predicted lipoprotein (NCBI) compare
b4551 yheV +0.6 2.6 hypothetical protein (NCBI) compare
b1557 cspB +0.6 1.6 Qin prophage; cold shock protein (NCBI) compare
b3206 npr +0.6 4.1 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1183 umuD +0.6 1.0 DNA polymerase V, subunit D (NCBI) compare
b3193 yrbD +0.6 4.7 predicted ABC-type organic solvent transporter (NCBI) compare
b3194 yrbE +0.6 4.6 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b3119 tdcR +0.6 2.3 threonine dehydratase operon activator protein (VIMSS) compare
b0728 sucC +0.6 3.8 succinyl-CoA synthetase subunit beta (NCBI) compare
b0294 ykgK +0.6 2.3 predicted regulator (NCBI) compare
b1453 ansP +0.6 2.5 L-asparagine permease (VIMSS) compare
b1148 ymfM +0.6 1.9 e14 prophage; predicted protein (NCBI) compare
b1384 feaR +0.5 3.2 DNA-binding transcriptional dual regulator (NCBI) compare
b1753 ynjA +0.5 2.1 hypothetical protein (NCBI) compare
b2286 nuoC +0.5 2.5 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b2279 nuoK +0.5 1.2 NADH dehydrogenase subunit K (NCBI) compare
b1793 yoaF +0.5 2.0 conserved outer membrane protein (NCBI) compare
b1620 malI +0.5 1.4 DNA-binding transcriptional repressor (NCBI) compare
b3281 aroE +0.5 4.7 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b1874 cutC +0.5 1.3 copper homeostasis protein (NCBI) compare
b1231 tyrT +0.5 0.9 tRNA-Tyr (NCBI) compare
b4592 yccB +0.5 1.6 hypothetical protein (RefSeq) compare
b1221 narL +0.5 3.1 DNA-binding response regulator in two-component regulatory system with NarX (or NarQ) (NCBI) compare
b1729 ydjN +0.5 2.5 predicted transporter (NCBI) compare
b1572 ydfB +0.5 1.3 orf, hypothetical protein (VIMSS) compare
b3844 fre +0.5 3.0 NAD(P)H-flavin reductase (NCBI) compare
b1756 ynjD +0.5 2.5 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b3260 dusB +0.5 2.1 tRNA-dihydrouridine synthase B (NCBI) compare
b3643 rph +0.5 3.3 ribonuclease PH (NCBI) compare
b3780 rhlB +0.5 4.1 ATP-dependent RNA helicase (NCBI) compare
b0729 sucD +0.5 2.5 succinyl-CoA synthetase subunit alpha (NCBI) compare
b3191 yrbB +0.5 3.8 orf, hypothetical protein (VIMSS) compare
b0632 dacA +0.5 3.5 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b3630 rfaP +0.5 4.0 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b1300 puuC +0.5 1.3 gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI) compare
b3181 greA +0.5 3.2 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b2280 nuoJ +0.5 2.5 NADH dehydrogenase subunit J (NCBI) compare
b0249 ykfF +0.5 1.7 CP4-6 prophage; predicted protein (NCBI) compare
b1108 ycfP +0.5 1.6 orf, hypothetical protein (VIMSS) compare
b1818 manY +0.5 1.2 mannose-specific enzyme IIC component of PTS (NCBI) compare
b0145 dksA +0.5 1.3 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b3198 kdsC +0.5 1.5 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b2071 yegJ +0.5 1.3 hypothetical protein (NCBI) compare
b2501 ppk +0.5 3.4 polyphosphate kinase (NCBI) compare
b1227 narI +0.5 2.5 nitrate reductase 1, gamma (cytochrome b(NR)) subunit (NCBI) compare
b3632 rfaQ +0.5 4.3 lipopolysaccharide core biosynthesis protein (NCBI) compare
b0471 ybaB +0.5 1.6 hypothetical protein (NCBI) compare
b0386 proC +0.5 2.3 pyrroline-5-carboxylate reductase (NCBI) compare
b1446 ydcY +0.5 1.0 hypothetical protein (NCBI) compare
b0436 tig +0.5 3.0 trigger factor (NCBI) compare
b2859 ygeO +0.5 1.1 hypothetical protein (RefSeq) compare
b1282 yciH +0.5 2.8 orf, hypothetical protein (VIMSS) compare
b1330 ynaI +0.5 2.1 conserved inner membrane protein (NCBI) compare
b1491 yddW +0.5 2.6 predicted liprotein (NCBI) compare
b0560 nohB +0.5 1.7 DLP12 prophage; DNA packaging protein (NCBI) compare
b0857 potI +0.4 1.7 putrescine transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1438 ydcQ +0.4 1.2 predicted DNA-binding transcriptional regulator (RefSeq) compare
b1550 b1550 +0.4 0.4 orf, hypothetical protein (VIMSS) compare
b0865 ybjP +0.4 1.4 predicted lipoprotein (NCBI) compare
b4152 frdC +0.4 2.6 fumarate reductase subunit C (NCBI) compare
b1682 sufC +0.4 2.8 cysteine desulfurase ATPase component (NCBI) compare
b3404 envZ +0.4 3.5 osmolarity sensor protein (NCBI) compare
b0726 sucA +0.4 1.9 alpha-ketoglutarate decarboxylase (NCBI) compare
b2277 nuoM +0.4 2.4 NADH dehydrogenase subunit M (NCBI) compare
b4203 rplI +0.4 1.8 50S ribosomal protein L9 (NCBI) compare
b4153 frdB +0.4 2.8 fumarate reductase (anaerobic), Fe-S subunit (NCBI) compare
b3784 rfe +0.4 3.5 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b2684 mprA +0.4 2.1 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b3842 rfaH +0.4 2.3 transcriptional activator RfaH (NCBI) compare
b1698 ydiR +0.4 1.7 predicted electron transfer flavoprotein, FAD-binding (NCBI) compare
b3890 yiiF +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b2477 nlpB +0.4 1.4 lipoprotein-34 (VIMSS) compare
b0945 pyrD +0.4 3.3 dihydroorotate dehydrogenase (NCBI) compare
b4578 insP +0.4 1.8 no description compare
b2085 b2085 +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b3631 rfaG +0.4 3.6 glucosyltransferase I (NCBI) compare
b2597 yfiA +0.4 1.2 cold shock protein associated with 30S ribosomal subunit (NCBI) compare
b1667 ydhR +0.4 1.2 hypothetical protein (NCBI) compare
b2564 pdxJ +0.4 1.6 pyridoxal phosphate biosynthetic protein (NCBI) compare
b2005 yeeV +0.4 1.8 CP4-44 prophage; toxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b1462 b1462 +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b3437 gntK +0.4 2.4 gluconokinase 2, thermoresistant (VIMSS) compare
b4057 yjbR +0.4 2.1 hypothetical protein (NCBI) compare
b0183 rnhB +0.4 1.8 ribonuclease HII (NCBI) compare
b1865 nudB +0.4 0.9 dATP pyrophosphohydrolase (NCBI) compare
b1726 yniB +0.4 1.1 predicted inner membrane protein (NCBI) compare
b3620 rfaF +0.4 2.1 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b1334 fnr +0.4 1.6 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b4481 rffT +0.4 2.4 4-alpha-L-fucosyltransferase (NCBI) compare
b1382 ynbE +0.4 0.8 predicted lipoprotein (NCBI) compare
b2792 yqcC +0.4 2.0 hypothetical protein (NCBI) compare
b1853 yebK +0.4 1.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b1970 yedX +0.4 1.6 hypothetical protein (NCBI) compare
b2808 gcvA +0.4 2.0 DNA-binding transcriptional dual regulator (NCBI) compare
b2399 yfeD +0.4 2.2 predicted DNA-binding transcriptional regulator (RefSeq) compare
b4694 yagP +0.4 2.0 no description compare
b1593 b1593 +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b4620 yjbT +0.4 2.1 hypothetical protein, no homologs (RefSeq) compare
b3867 hemN +0.4 3.2 O2-independent coproporphyrinogen III oxidase (VIMSS) compare
b0685 ybfE +0.4 0.8 orf, hypothetical protein (VIMSS) compare
b3790 rffC +0.4 2.1 TDP-fucosamine acetyltransferase (RefSeq) compare
b2579 yfiD +0.4 0.7 pyruvate formate lyase subunit (NCBI) compare
b2145 yeiS +0.4 1.2 predicted inner membrane protein (NCBI) compare
b2768 ygcP +0.4 1.3 predicted anti-terminator regulatory protein (NCBI) compare
b2419 yfeK +0.4 0.9 hypothetical protein (NCBI) compare
b1412 azoR +0.4 1.5 acyl carrier protein phosphodiesterase (NCBI) compare
b2215 ompC +0.4 2.4 outer membrane porin protein C (NCBI) compare
b4589 ylcI +0.4 1.4 hypothetical protein (RefSeq) compare
b2424 cysU +0.4 0.9 sulfate/thiosulfate transporter subunit (NCBI) compare
b0226 dinJ +0.4 2.2 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b3622 rfaL +0.4 1.3 O-antigen ligase (NCBI) compare
b0889 lrp +0.4 1.6 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b3642 pyrE +0.4 1.6 orotate phosphoribosyltransferase (NCBI) compare
b3448 yhhA +0.4 1.7 hypothetical protein (NCBI) compare
b2912 ygfA +0.4 1.4 putative ligase (VIMSS) compare
b1153 ymfQ +0.4 1.0 e14 prophage; conserved protein (NCBI) compare
b1639 ydhA +0.4 2.1 orf, hypothetical protein (VIMSS) compare
b1559 b1559 +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b1083 flgL +0.4 1.5 flagellar hook-associated protein L (NCBI) compare
b1251 yciI +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b0354 yaiL +0.4 2.2 nucleoprotein/polynucleotide-associated enzyme (VIMSS) compare
b2136 yohD +0.4 0.5 orf, hypothetical protein (VIMSS) compare
b0357 yaiN +0.3 0.8 putative alpha helix chain (VIMSS) compare
b1175 minD +0.3 1.9 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b4458 oxyS +0.3 0.7 global regulatory RNA (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Cholinium glutamate across organisms