Experiment set17IT055 for Escherichia coli BW25113

Compare to:

LB with e-Amino-N-Caproic Acid 10 vol%

200 most detrimental genes:

  gene name fitness t score description  
b0721 sdhC +2.1 7.4 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b1192 ldcA +2.0 7.9 L,D-carboxypeptidase A (NCBI) compare
b0723 sdhA +1.9 13.2 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b0033 carB +1.9 16.0 carbamoyl-phosphate synthase large subunit (NCBI) compare
b0724 sdhB +1.8 10.2 succinate dehydrogenase, FeS subunit (NCBI) compare
b2281 nuoI +1.8 6.5 NADH dehydrogenase subunit I (NCBI) compare
b0032 carA +1.7 8.5 carbamoyl-phosphate synthase small subunit (NCBI) compare
b0232 yafN +1.6 1.5 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b3236 mdh +1.6 11.7 malate dehydrogenase (NCBI) compare
b4146 yjeK +1.6 6.2 predicted lysine aminomutase (NCBI) compare
b3844 fre +1.6 10.7 NAD(P)H-flavin reductase (NCBI) compare
b0722 sdhD +1.6 7.6 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b2278 nuoL +1.5 10.6 NADH dehydrogenase subunit L (NCBI) compare
b2287 nuoB +1.5 5.5 NADH dehydrogenase subunit B (NCBI) compare
b2276 nuoN +1.4 8.7 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b4313 fimE +1.4 9.1 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b4552 yrhC +1.4 1.8 no description compare
b2283 nuoG +1.4 9.5 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b2285 nuoE +1.4 4.3 NADH dehydrogenase subunit E (NCBI) compare
b2286 nuoC +1.4 6.9 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b0726 sucA +1.3 6.5 alpha-ketoglutarate decarboxylase (NCBI) compare
b2277 nuoM +1.3 8.3 NADH dehydrogenase subunit M (NCBI) compare
b2829 ptsP +1.3 9.9 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b2897 ygfY +1.3 7.8 hypothetical protein (NCBI) compare
b1293 sapB +1.2 2.0 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2280 nuoJ +1.2 6.1 NADH dehydrogenase subunit J (NCBI) compare
b3198 kdsC +1.2 4.7 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b1438 ydcQ +1.2 3.3 predicted DNA-binding transcriptional regulator (RefSeq) compare
b2279 nuoK +1.2 3.3 NADH dehydrogenase subunit K (NCBI) compare
b0049 apaH +1.1 5.4 diadenosinetetraphosphatase (NCBI) compare
b3806 cyaA +1.1 6.1 adenylate cyclase (NCBI) compare
b2284 nuoF +1.1 5.5 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b2501 ppk +1.1 7.7 polyphosphate kinase (NCBI) compare
b2282 nuoH +1.0 4.1 NADH dehydrogenase subunit H (NCBI) compare
b2512 yfgL +1.0 6.0 protein assembly complex, lipoprotein component (NCBI) compare
b1085 b1085 +1.0 3.4 orf, hypothetical protein (VIMSS) compare
b3199 yrbK +1.0 4.4 hypothetical protein (NCBI) compare
b4418 sraB +1.0 2.2 unknown RNA (NCBI) compare
b3341 rpsG +1.0 1.2 30S ribosomal protein S7 (NCBI) compare
b3643 rph +1.0 4.4 ribonuclease PH (NCBI) compare
b2531 iscR +0.9 1.4 DNA-binding transcriptional repressor (NCBI) compare
b0720 gltA +0.9 6.4 citrate synthase (NCBI) compare
b3636 rpmG +0.9 1.6 50S ribosomal protein L33 (NCBI) compare
b3620 rfaF +0.9 4.8 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3405 ompR +0.9 4.2 osmolarity response regulator (NCBI) compare
b1062 pyrC +0.9 5.7 dihydroorotase (NCBI) compare
b4481 rffT +0.8 4.2 4-alpha-L-fucosyltransferase (NCBI) compare
b4232 fbp +0.8 6.9 fructose-1,6-bisphosphatase (NCBI) compare
b3842 rfaH +0.8 4.6 transcriptional activator RfaH (NCBI) compare
b4585 sroB +0.8 1.5 Novel sRNA, function unknown (RefSeq) compare
b0728 sucC +0.8 4.6 succinyl-CoA synthetase subunit beta (NCBI) compare
b1874 cutC +0.8 2.6 copper homeostasis protein (NCBI) compare
b3164 pnp +0.8 2.3 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b0934 ycbM +0.8 3.1 putative transport system permease protein (VIMSS) compare
b1553 ydfP +0.8 2.8 Qin prophage; conserved protein (NCBI) compare
b3484 yhhI +0.8 2.5 predicted transposase (NCBI) compare
b2659 b2659 +0.8 2.7 orf, hypothetical protein (VIMSS) compare
b2141 yohJ +0.8 3.2 hypothetical protein (NCBI) compare
b4592 yccB +0.7 2.0 hypothetical protein (RefSeq) compare
b2642 yfjW +0.7 0.7 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b0729 sucD +0.7 3.8 succinyl-CoA synthetase subunit alpha (NCBI) compare
b3239 yhcO +0.7 2.4 predicted barnase inhibitor (NCBI) compare
b1058 yceO +0.7 0.4 hypothetical protein (NCBI) compare
b4416 rybA +0.7 1.4 unknown RNA (NCBI) compare
b1446 ydcY +0.7 2.2 hypothetical protein (NCBI) compare
b2684 mprA +0.7 2.7 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b3619 rfaD +0.7 2.5 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3238 yhcN +0.7 1.9 orf, hypothetical protein (VIMSS) compare
b4557 yidD +0.7 2.0 hypothetical protein (NCBI) compare
b3404 envZ +0.7 4.8 osmolarity sensor protein (NCBI) compare
b2516 yfgA +0.7 3.2 hypothetical protein (NCBI) compare
b2288 nuoA +0.7 1.9 NADH dehydrogenase I chain A (VIMSS) compare
b0739 tolA +0.6 2.4 cell envelope integrity inner membrane protein TolA (NCBI) compare
b2393 nupC +0.6 4.0 nucleoside (except guanosine) transporter (NCBI) compare
b3163 nlpI +0.6 2.8 hypothetical protein (NCBI) compare
b1830 prc +0.6 3.1 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b2433 b2433 +0.6 1.4 orf, hypothetical protein (VIMSS) compare
b1856 yebA +0.6 4.7 orf, hypothetical protein (VIMSS) compare
b4128 yjdK +0.6 0.4 hypothetical protein (NCBI) compare
b0303 ykgI +0.6 0.7 orf, hypothetical protein (VIMSS) compare
b1550 b1550 +0.6 1.1 orf, hypothetical protein (VIMSS) compare
b3387 dam +0.6 5.0 DNA adenine methylase (NCBI) compare
b3250 mreC +0.6 0.8 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b2741 rpoS +0.6 2.8 RNA polymerase sigma factor (NCBI) compare
b3766 ilvL +0.6 2.8 ilvG operon leader peptide (NCBI) compare
b1434 ydcN +0.6 1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b4203 rplI +0.6 2.5 50S ribosomal protein L9 (NCBI) compare
b0436 tig +0.6 4.2 trigger factor (NCBI) compare
b2990 hybG +0.6 1.4 hydrogenase 2 accessory protein (NCBI) compare
b3193 yrbD +0.6 4.0 predicted ABC-type organic solvent transporter (NCBI) compare
b1914 uvrY +0.6 2.8 response regulator (NCBI) compare
b1627 rsxA +0.6 1.7 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b0912 ihfB +0.6 2.6 integration host factor subunit beta (NCBI) compare
b1583 ynfB +0.6 2.5 hypothetical protein (NCBI) compare
b0737 tolQ +0.6 1.4 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3029 ygiN +0.6 1.7 quinol monooxygenase (NCBI) compare
b0623 cspE +0.6 1.4 cold shock protein E (NCBI) compare
b1143 ymfI +0.6 1.8 orf, hypothetical protein (VIMSS) compare
b1074 flgC +0.6 1.7 flagellar basal-body rod protein C (NCBI) compare
b1540 ydfH +0.6 1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b2061 wzb +0.6 1.9 protein-tyrosine phosphatase (NCBI) compare
b3791 rffA +0.6 3.3 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b3194 yrbE +0.6 4.2 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1111 ycfQ +0.6 1.9 orf, hypothetical protein (VIMSS) compare
b1514 lsrC +0.5 1.7 AI2 transporter (NCBI) compare
b1147 ymfL +0.5 1.4 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b3191 yrbB +0.5 3.6 orf, hypothetical protein (VIMSS) compare
b2673 nrdH +0.5 2.7 glutaredoxin-like protein (NCBI) compare
b4402 yjjY +0.5 0.5 hypothetical protein (NCBI) compare
b1503 ydeR +0.5 2.0 predicted fimbrial-like adhesin protein (NCBI) compare
b4075 nrfF +0.5 2.6 heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfF (NCBI) compare
b4090 rpiB +0.5 2.1 ribose-5-phosphate isomerase B (NCBI) compare
b0222 lpcA +0.5 1.5 phosphoheptose isomerase (NCBI) compare
b2215 ompC +0.5 3.5 outer membrane porin protein C (NCBI) compare
b3206 npr +0.5 2.7 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1837 yebW +0.5 2.0 orf, hypothetical protein (VIMSS) compare
b1823 cspC +0.5 1.6 stress protein, member of the CspA-family (NCBI) compare
b3192 yrbC +0.5 3.6 predicted ABC-type organic solvent transporter (NCBI) compare
b1891 flhC +0.5 1.9 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b4407 thiS +0.5 1.1 sulfur carrier protein ThiS (NCBI) compare
b0014 dnaK +0.5 1.3 molecular chaperone DnaK (NCBI) compare
b1171 b1171 +0.5 1.6 orf, hypothetical protein (VIMSS) compare
b0985 ymcB +0.5 2.9 hypothetical protein (NCBI) compare
b1422 ydcI +0.5 1.6 putative transcriptional regulator LYSR-type (VIMSS) compare
b0632 dacA +0.5 3.0 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b2700 ygaD +0.5 1.7 competence damage-inducible protein A (NCBI) compare
b0740 tolB +0.5 2.5 translocation protein TolB precursor (NCBI) compare
b0682 ybfN +0.5 2.1 predicted lipoprotein (NCBI) compare
b4529 ydbJ +0.5 0.7 hypothetical protein (NCBI) compare
b2698 recX +0.5 1.0 RecA regulator RecX (NCBI) compare
b1992 cobS +0.5 1.5 cobalamin synthase (NCBI) compare
b0535 fimZ +0.5 2.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b1565 ydfV +0.5 2.0 Qin prophage; predicted protein (NCBI) compare
b2017 yefM +0.5 0.6 orf, hypothetical protein (VIMSS) compare
b1819 manZ +0.5 1.9 mannose-specific enzyme IID component of PTS (NCBI) compare
b1991 cobT +0.5 2.1 nicotinate-nucleotide--dimethylbenzimidazolephosphoribosyltransferase (NCBI) compare
b0945 pyrD +0.5 3.5 dihydroorotate dehydrogenase (NCBI) compare
b1507 hipA +0.5 2.2 regulator with hipB (NCBI) compare
b3621 rfaC +0.5 2.4 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b1661 cfa +0.5 1.9 cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI) compare
b1220 ychO +0.4 2.1 predicted invasin (RefSeq) compare
b0738 tolR +0.4 1.6 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0746 valZ +0.4 1.3 tRNA-Val (NCBI) compare
b1792 yeaO +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b3210 arcB +0.4 2.8 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b4543 ypaA +0.4 2.2 predicted protein (NCBI) compare
b1247 oppF +0.4 2.4 oligopeptide transporter subunit (NCBI) compare
b2939 yqgB +0.4 0.8 predicted protein (RefSeq) compare
b3120 yhaB +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b3195 yrbF +0.4 3.6 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b1348 lar +0.4 1.1 Rac prophage; restriction alleviation protein (NCBI) compare
b1249 cls +0.4 2.4 cardiolipin synthetase (NCBI) compare
b4595 yciY +0.4 0.7 hypothetical protein (RefSeq) compare
b0708 phr +0.4 2.6 deoxyribodipyrimidine photolyase, FAD-binding (NCBI) compare
b2538 hcaE +0.4 2.2 3-phenylpropionate dioxygenase, large (alpha) subunit (NCBI) compare
b1048 mdoG +0.4 2.7 glucan biosynthesis protein, periplasmic (NCBI) compare
b3780 rhlB +0.4 3.5 ATP-dependent RNA helicase (NCBI) compare
b2674 nrdI +0.4 2.7 hypothetical protein (NCBI) compare
b4544 yfbW +0.4 1.9 hypothetical protein (NCBI) compare
b0065 yabI +0.4 1.7 conserved inner membrane protein (NCBI) compare
b3630 rfaP +0.4 3.3 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b2617 smpA +0.4 1.8 small membrane protein A (VIMSS) compare
b1144 ymfJ +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b1936 intG +0.4 0.7 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1989 asnV +0.4 1.3 tRNA-Asn (NCBI) compare
b0220 ivy +0.4 2.2 inhibitor of vertebrate C-lysozyme (NCBI) compare
b4527 b1354 +0.4 2.0 orf, hypothetical protein (VIMSS) compare
b1291 sapD +0.4 0.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0556 rzpD +0.4 1.9 DLP12 prophage; predicted murein endopeptidase (NCBI) compare
b0857 potI +0.4 2.4 putrescine transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1821 b1821 +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b2946 yggJ +0.4 2.1 orf, hypothetical protein (VIMSS) compare
b2083 yegZ +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b4410 ecnA +0.4 0.9 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b1134 ymfB +0.4 0.8 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b1804 rnd +0.4 1.9 ribonuclease D (NCBI) compare
b1608 rstA +0.4 1.9 DNA-binding response regulator in two-component regulatory system with RstB (RefSeq) compare
b2049 cpsB +0.4 2.2 mannose-1-phosphate guanyltransferase (NCBI) compare
b3594 yibA +0.4 2.7 lyase containing HEAT-repeat (NCBI) compare
b3348 slyX +0.4 1.8 hypothetical protein (NCBI) compare
b2186 yejK +0.4 3.4 nucleoid-associated protein NdpA (NCBI) compare
b1699 ydiS +0.4 1.6 predicted oxidoreductase with FAD/NAD(P)-binding domain (NCBI) compare
b2430 b2430 +0.4 2.3 putative beta-lactamase (VIMSS) compare
b1954 dsrA +0.4 0.7 regulatory antisense RNA (NCBI) compare
b4215 ytfI +0.4 0.8 hypothetical protein (NCBI) compare
b1930 yedF +0.4 2.2 hypothetical protein (NCBI) compare
b2809 b2809 +0.4 0.8 orf, hypothetical protein (VIMSS) compare
b0848 ybjM +0.4 2.3 predicted inner membrane protein (NCBI) compare
b2110 yehC +0.4 0.6 predicted periplasmic pilin chaperone (NCBI) compare
b1628 rsxB +0.4 0.9 electron transport complex protein RnfB (NCBI) compare
b1400 paaY +0.4 1.3 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b2506 yfgI +0.4 1.4 hypothetical protein (NCBI) compare
b0937 ssuE +0.4 1.2 NAD(P)H-dependent FMN reductase (NCBI) compare
b1412 azoR +0.4 1.2 acyl carrier protein phosphodiesterase (NCBI) compare
b4307 yjhQ +0.4 2.7 KpLE2 phage-like element; predicted acetyltransferase (NCBI) compare
b1141 ymfG +0.4 1.4 e14 prophage; predicted excisionase (NCBI) compare
b4391 yjjK +0.4 2.7 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b0505 allA +0.4 1.0 ureidoglycolate hydrolase (NCBI) compare
b1480 sra +0.4 0.4 30S ribosomal subunit protein S22 (NCBI) compare
b4594 ymgJ +0.4 1.2 hypothetical protein (RefSeq) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress e-Amino-N-Caproic Acid across organisms