Experiment set17IT048 for Escherichia coli BW25113

Compare to:

LB with Caprolactam 7 vol%

200 most important genes:

  gene name fitness t score description  
b0149 mrcB -4.1 -13.9 penicillin-binding protein 1b (NCBI) compare
b3792 wzxE -3.9 -4.6 O-antigen translocase (NCBI) compare
b3839 tatC -3.7 -6.2 TatABCE protein translocation system subunit (NCBI) compare
b3398 yrfF -3.5 -3.4 predicted inner membrane protein (NCBI) compare
b1105 ycfM -3.1 -5.8 predicted outer membrane lipoprotein (NCBI) compare
b3052 rfaE -2.7 -7.7 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3493 pitA -2.7 -8.8 phosphate transporter, low-affinity (NCBI) compare
b3405 ompR -2.5 -6.5 osmolarity response regulator (NCBI) compare
b1236 galU -2.3 -4.8 glucose-1-phosphate uridylyltransferase (NCBI) compare
b0740 tolB -2.3 -6.2 translocation protein TolB precursor (NCBI) compare
b3621 rfaC -2.2 -7.0 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3619 rfaD -2.1 -4.7 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3979 thrT -2.1 -1.0 tRNA-Thr (NCBI) compare
b3204 ptsN -2.0 -6.9 sugar-specific enzyme IIA component of PTS (NCBI) compare
b0049 apaH -2.0 -5.2 diadenosinetetraphosphatase (NCBI) compare
b3620 rfaF -1.8 -6.8 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b0438 clpX -1.8 -8.3 ATP-dependent protease ATP-binding subunit (NCBI) compare
b2688 gshA -1.8 -8.9 glutamate--cysteine ligase (NCBI) compare
b3426 glpD -1.8 -3.5 sn-glycerol-3-phosphate dehydrogenase, aerobic, FAD/NAD(P)-binding (NCBI) compare
b3290 trkA -1.7 -1.2 potassium transporter peripheral membrane component (NCBI) compare
b3650 spoT -1.7 -5.2 bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI) compare
b0437 clpP -1.7 -6.7 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b0630 lipB -1.7 -1.1 protein of lipoate biosynthesis (VIMSS) compare
b1861 ruvA -1.6 -1.7 Holliday junction DNA helicase motor protein (NCBI) compare
b0739 tolA -1.6 -4.0 cell envelope integrity inner membrane protein TolA (NCBI) compare
b0014 dnaK -1.6 -2.0 molecular chaperone DnaK (NCBI) compare
b1765 ydjA -1.6 -1.8 predicted oxidoreductase (NCBI) compare
b2498 upp -1.6 -3.5 uracil phosphoribosyltransferase (VIMSS) compare
b0222 lpcA -1.6 -3.5 phosphoheptose isomerase (NCBI) compare
b3863 polA -1.6 -1.5 DNA polymerase I (NCBI) compare
b3026 qseC -1.5 -4.7 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b1095 fabF -1.4 -7.8 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b3035 tolC -1.4 -7.8 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b4201 priB -1.4 -2.3 primosomal replication protein N (NCBI) compare
b1882 cheY -1.4 -0.9 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1290 sapF -1.3 -2.0 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1361 ydaW -1.3 -1.0 orf, hypothetical protein (VIMSS) compare
b1677 lpp -1.3 -3.6 murein lipoprotein (NCBI) compare
b1493 gadB -1.3 -1.2 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b1292 sapC -1.3 -2.4 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1291 sapD -1.3 -1.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2433 b2433 -1.3 -2.3 orf, hypothetical protein (VIMSS) compare
b2259 pmrD -1.3 -2.1 polymyxin resistance protein B (VIMSS) compare
b2830 nudH -1.2 -0.8 dinucleoside polyphosphate hydrolase (NCBI) compare
b3842 rfaH -1.2 -5.6 transcriptional activator RfaH (NCBI) compare
b3838 tatB -1.2 -5.5 sec-independent translocase (NCBI) compare
b3390 aroK -1.2 -1.7 shikimate kinase I (NCBI) compare
b2288 nuoA -1.2 -2.2 NADH dehydrogenase I chain A (VIMSS) compare
b0200 gmhB -1.1 -1.2 hypothetical protein (NCBI) compare
b1860 ruvB -1.1 -0.7 Holliday junction DNA helicase B (NCBI) compare
b4503 yafF -1.1 -1.0 no description compare
b1258 yciF -1.1 -0.7 hypothetical protein (NCBI) compare
b4202 rpsR -1.1 -1.0 30S ribosomal protein S18 (NCBI) compare
b1358 ydaT -1.1 -1.8 Rac prophage; predicted protein (NCBI) compare
b3414 gntY -1.0 -4.2 predicted gluconate transport associated protein (NCBI) compare
b0439 lon -1.0 -3.2 DNA-binding ATP-dependent protease La (NCBI) compare
b0741 pal -1.0 -3.2 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b1161 ycgX -1.0 -0.9 hypothetical protein (NCBI) compare
b2531 iscR -1.0 -1.4 DNA-binding transcriptional repressor (NCBI) compare
b3389 aroB -1.0 -6.9 3-dehydroquinate synthase (NCBI) compare
b1476 fdnI -1.0 -1.4 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b2318 truA -1.0 -3.1 tRNA pseudouridine synthase A (NCBI) compare
b0738 tolR -1.0 -2.3 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b4508 peaD -1.0 -1.5 putative sensory transduction regulator (VIMSS) compare
b3630 rfaP -1.0 -6.4 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b1058 yceO -0.9 -0.5 hypothetical protein (NCBI) compare
b1235 rssB -0.9 -4.9 response regulator of RpoS (NCBI) compare
b4593 ymgI -0.9 -0.6 hypothetical protein (RefSeq) compare
b2947 gshB -0.9 -3.1 glutathione synthetase (NCBI) compare
b4208 cycA -0.9 -6.3 D-alanine/D-serine/glycine transporter (NCBI) compare
b0758 galT -0.9 -5.0 galactose-1-phosphate uridylyltransferase (NCBI) compare
b4191 ulaR -0.9 -3.5 DNA-binding transcriptional dual regulator (NCBI) compare
b1506 yneL -0.9 -1.2 predicted transcriptional regulator (NCBI) compare
b0726 sucA -0.9 -2.6 alpha-ketoglutarate decarboxylase (NCBI) compare
b4222 ytfP -0.9 -3.6 hypothetical protein (NCBI) compare
b0247 ykfG -0.9 -1.8 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b4431 rprA -0.9 -2.2 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b1084 rne -0.9 -3.3 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b2699 recA -0.9 -0.6 recombinase A (NCBI) compare
b1351 racC -0.9 -0.5 Rac prophage; predicted protein (NCBI) compare
b4146 yjeK -0.9 -2.2 predicted lysine aminomutase (NCBI) compare
b4585 sroB -0.8 -1.5 Novel sRNA, function unknown (RefSeq) compare
b0019 nhaA -0.8 -5.8 pH-dependent sodium/proton antiporter (NCBI) compare
b1557 cspB -0.8 -2.4 Qin prophage; cold shock protein (NCBI) compare
b3387 dam -0.8 -6.7 DNA adenine methylase (NCBI) compare
b2340 sixA -0.8 -3.3 phosphohistidine phosphatase (NCBI) compare
b0548 ninE -0.8 -2.5 DLP12 prophage; conserved protein (NCBI) compare
b0028 fkpB -0.8 -2.0 FKBP-type peptidyl-prolyl cis-trans isomerase (rotamase) (NCBI) compare
b2518 ndk -0.8 -1.9 nucleoside diphosphate kinase (NCBI) compare
b3649 rpoZ -0.8 -2.4 DNA-directed RNA polymerase subunit omega (NCBI) compare
b0145 dksA -0.8 -2.7 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b1556 b1556 -0.8 -2.1 orf, hypothetical protein (VIMSS) compare
b3281 aroE -0.8 -6.8 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b2906 visC -0.8 -4.3 hypothetical protein (NCBI) compare
b3631 rfaG -0.8 -6.5 glucosyltransferase I (NCBI) compare
b0890 ftsK -0.8 -2.4 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b1497 b1497 -0.8 -0.4 putative enzyme (VIMSS) compare
b4613 dinQ -0.8 -1.5 Damage inducible, function unknown (RefSeq) compare
b1499 ydeO -0.8 -0.4 predicted DNA-binding transcriptional acfivator (NCBI) compare
b1551 b1551 -0.8 -0.4 orf, hypothetical protein (VIMSS) compare
b1576 ydfD -0.8 -0.4 Qin prophage; predicted protein (NCBI) compare
b3149 yraO -0.8 -3.8 DnaA initiator-associating factor for replication initiation (NCBI) compare
b1147 ymfL -0.8 -1.9 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b1363 trkG -0.8 -0.4 Rac prophage; potassium transporter subunit (NCBI) compare
b2673 nrdH -0.8 -3.3 glutaredoxin-like protein (NCBI) compare
b1347 ydaC -0.8 -0.4 Rac prophage; predicted protein (NCBI) compare
b1545 pinQ -0.7 -0.3 Qin prophage; predicted site-specific recombinase (NCBI) compare
b3836 b3836 -0.7 -2.5 orf, hypothetical protein (VIMSS) compare
b1968 yedV -0.7 -2.0 predicted sensory kinase in two-component regulatory system with YedW (NCBI) compare
b0888 trxB -0.7 -2.4 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b0431 cyoB -0.7 -5.6 cytochrome o ubiquinol oxidase subunit I (NCBI) compare
b2325 yfcL -0.7 -3.1 hypothetical protein (NCBI) compare
b0080 fruR -0.7 -3.8 DNA-binding transcriptional dual regulator (NCBI) compare
b0535 fimZ -0.7 -2.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b4509 ylcG -0.7 -2.2 DLP12 prophage; predicted protein (NCBI) compare
b1465 narV -0.7 -2.2 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b1257 yciE -0.7 -0.3 hypothetical protein (NCBI) compare
b2282 nuoH -0.7 -2.2 NADH dehydrogenase subunit H (NCBI) compare
b3150 yraP -0.7 -3.2 hypothetical protein (NCBI) compare
b1658 purR -0.7 -4.0 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b4685 yrbN -0.7 -1.9 expressed protein (RefSeq) compare
b1693 aroD -0.7 -3.7 3-dehydroquinate dehydratase (NCBI) compare
b1878 flhE -0.7 -3.0 hypothetical protein (NCBI) compare
b1129 phoQ -0.7 -1.1 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b1324 tpx -0.7 -3.2 thiol peroxidase (NCBI) compare
b1090 plsX -0.7 -3.1 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b3849 trkH -0.7 -5.1 potassium transporter (NCBI) compare
b2659 b2659 -0.7 -1.4 orf, hypothetical protein (VIMSS) compare
b1182 hlyE -0.7 -0.3 hemolysin E (VIMSS) compare
b4178 yjeB -0.7 -3.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b3652 recG -0.7 -4.0 ATP-dependent DNA helicase (NCBI) compare
b3933 ftsN -0.7 -5.0 essential cell division protein (NCBI) compare
b1792 yeaO -0.7 -2.8 orf, hypothetical protein (VIMSS) compare
b1176 minC -0.7 -1.8 septum formation inhibitor (NCBI) compare
b2329 aroC -0.6 -2.8 chorismate synthase (NCBI) compare
b1730 ydjO -0.6 -0.8 predicted protein (RefSeq) compare
b1167 ymgC -0.6 -0.3 hypothetical protein (NCBI) compare
b4451 ryhB -0.6 -1.4 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b1165 ymgA -0.6 -0.3 hypothetical protein (NCBI) compare
b4497 yeeL -0.6 -0.7 no description compare
b3742 mioC -0.6 -3.0 flavodoxin (NCBI) compare
b1530 marR -0.6 -2.2 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b2683 ygaH -0.6 -3.6 predicted inner membrane protein (NCBI) compare
b0430 cyoC -0.6 -2.2 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b1505 ydeT -0.6 -1.0 hypothetical protein (RefSeq) compare
b3613 yibP -0.6 -1.6 putative membrane protein (VIMSS) compare
b3199 yrbK -0.6 -1.4 hypothetical protein (NCBI) compare
b1582 ynfA -0.6 -1.0 hypothetical protein (NCBI) compare
b0429 cyoD -0.6 -3.1 cytochrome o ubiquinol oxidase subunit IV (NCBI) compare
b1847 yebF -0.6 -0.9 predicted protein (RefSeq) compare
b2055 wcaE -0.6 -0.3 predicted glycosyl transferase (NCBI) compare
b1977 asnT -0.6 -2.1 tRNA-Asn (NCBI) compare
b3205 yhbJ -0.6 -2.4 hypothetical protein (NCBI) compare
b4294 insA-7 -0.6 -1.6 KpLE2 phage-like element; IS1 repressor protein InsA (NCBI) compare
b2620 smpB -0.6 -0.8 SsrA-binding protein (NCBI) compare
b2707 srlR -0.6 -4.2 DNA-bindng transcriptional repressor (NCBI) compare
b1001 yccE -0.6 -0.3 hypothetical protein (NCBI) compare
b1524 yneH -0.6 -0.9 predicted glutaminase (NCBI) compare
b2143 cdd -0.6 -3.0 cytidine deaminase (NCBI) compare
b3791 rffA -0.6 -3.5 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b0627 tatE -0.6 -1.3 twin arginine translocase protein E (NCBI) compare
b4436 ryeC -0.6 -1.5 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b0623 cspE -0.6 -1.4 cold shock protein E (NCBI) compare
b4407 thiS -0.6 -1.3 sulfur carrier protein ThiS (NCBI) compare
b3609 secB -0.6 -1.8 export protein SecB (NCBI) compare
b2450 yffS -0.6 -0.9 CPZ-55 prophage; predicted protein (RefSeq) compare
b4413 sokC -0.6 -1.1 regulatory antisense RNA (NCBI) compare
b4634 ylcF -0.6 -2.5 no description compare
b1553 ydfP -0.6 -1.9 Qin prophage; conserved protein (NCBI) compare
b3169 nusA -0.6 -1.2 transcription elongation factor NusA (NCBI) compare
b1573 ydfC -0.6 -1.2 Qin prophage; predicted protein (RefSeq) compare
b4527 b1354 -0.6 -1.9 orf, hypothetical protein (VIMSS) compare
b0427 yajR -0.6 -4.1 putative transport protein (VIMSS) compare
b1409 ynbB -0.6 -0.6 predicted CDP-diglyceride synthase (NCBI) compare
b0218 yafU -0.5 -1.6 predicted inner membrane protein (RefSeq) compare
b0161 degP -0.5 -2.5 serine endoprotease (protease Do), membrane-associated (NCBI) compare
b3119 tdcR -0.5 -1.9 threonine dehydratase operon activator protein (VIMSS) compare
b1536 ydeI -0.5 -0.6 hypothetical protein (NCBI) compare
b1877 yecT -0.5 -1.3 orf, hypothetical protein (VIMSS) compare
b4568 ytjA -0.5 -1.6 predicted protein (RefSeq) compare
b3388 damX -0.5 -4.6 hypothetical protein (NCBI) compare
b3911 cpxA -0.5 -3.8 two-component sensor protein (NCBI) compare
b0428 cyoE -0.5 -3.4 protoheme IX farnesyltransferase (NCBI) compare
b3465 yhhF -0.5 -3.0 predicted methyltransferase (NCBI) compare
b1522 yneF -0.5 -2.1 predicted diguanylate cyclase (NCBI) compare
b0676 nagC -0.5 -3.5 DNA-binding transcriptional dual regulator, repressor of N-acetylglucosamine (NCBI) compare
b1548 nohA -0.5 -1.4 Qin prophage; predicted packaging protein (RefSeq) compare
b2614 grpE -0.5 -1.5 heat shock protein (NCBI) compare
b1830 prc -0.5 -2.4 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b3226 yhcK -0.5 -2.6 putative FADA-type transcriptional regulator (VIMSS) compare
b2297 pta -0.5 -0.3 phosphate acetyltransferase (NCBI) compare
b0737 tolQ -0.5 -1.3 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1025 ycdT -0.5 -1.0 predicted diguanylate cyclase (NCBI) compare
b1370 insH-5 -0.5 -1.3 Rac prophage; IS5 transposase and trans-activator (NCBI) compare
b2263 yfbB -0.5 -2.0 predicted peptidase (NCBI) compare
b0432 cyoA -0.5 -3.9 cytochrome o ubiquinol oxidase subunit II (NCBI) compare
b1192 ldcA -0.5 -1.9 L,D-carboxypeptidase A (NCBI) compare
b0015 dnaJ -0.5 -3.5 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b0912 ihfB -0.5 -1.8 integration host factor subunit beta (NCBI) compare
b0759 galE -0.5 -2.5 UDP-galactose-4-epimerase (VIMSS) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Caprolactam across organisms