Experiment set17IT045 for Escherichia coli BW25113

Compare to:

LB with Isobutyl alcohol 1.25 vol%

200 most detrimental genes:

  gene name fitness t score description  
b0462 acrB +4.5 43.4 multidrug efflux system protein (NCBI) compare
b0463 acrA +4.4 25.8 multidrug efflux system (NCBI) compare
b4396 rob +1.8 14.4 DNA-binding transcriptional activator (NCBI) compare
b1831 proQ +1.6 5.7 putative solute/DNA competence effector (NCBI) compare
b1954 dsrA +1.3 5.1 regulatory antisense RNA (NCBI) compare
b3163 nlpI +1.2 5.8 hypothetical protein (NCBI) compare
b3784 rfe +1.1 9.6 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b1130 phoP +1.1 2.4 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b3790 rffC +1.1 6.1 TDP-fucosamine acetyltransferase (RefSeq) compare
b1480 sra +1.1 2.5 30S ribosomal subunit protein S22 (NCBI) compare
b3183 obgE +1.1 1.6 GTPase involved in cell partioning and DNA repair (NCBI) compare
b3786 rffE +1.1 9.1 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b3791 rffA +1.0 7.9 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b1778 yeaA +1.0 3.9 methionine sulfoxide reductase B (NCBI) compare
b1830 prc +1.0 5.9 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b1642 slyA +1.0 3.4 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b1575 dicB +1.0 2.2 Qin prophage; cell division inhibition protein (NCBI) compare
b1914 uvrY +0.9 5.4 response regulator (NCBI) compare
b0014 dnaK +0.9 2.0 molecular chaperone DnaK (NCBI) compare
b3794 rffM +0.9 7.4 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b0723 sdhA +0.9 7.9 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b3236 mdh +0.9 7.7 malate dehydrogenase (NCBI) compare
b4481 rffT +0.9 6.2 4-alpha-L-fucosyltransferase (NCBI) compare
b4139 aspA +0.9 6.7 aspartate ammonia-lyase (aspartase) (VIMSS) compare
b3787 rffD +0.8 6.8 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b0580 ybdJ +0.8 2.0 predicted inner membrane protein (NCBI) compare
b1824 yobF +0.8 2.4 hypothetical protein (NCBI) compare
b3472 b3472 +0.8 6.0 orf, hypothetical protein (VIMSS) compare
b1949 fliQ +0.8 2.2 flagellar biosynthesis protein Q (NCBI) compare
b0724 sdhB +0.8 5.0 succinate dehydrogenase, FeS subunit (NCBI) compare
b0721 sdhC +0.8 3.2 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b3035 tolC +0.8 5.2 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b0632 dacA +0.8 5.2 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b1048 mdoG +0.8 4.7 glucan biosynthesis protein, periplasmic (NCBI) compare
b3644 yicC +0.7 4.9 hypothetical protein (NCBI) compare
b3363 ppiA +0.7 5.6 peptidyl-prolyl cis-trans isomerase A (rotamase A) (NCBI) compare
b2262 menB +0.7 3.4 naphthoate synthase (NCBI) compare
b4217 ytfK +0.7 4.3 orf, hypothetical protein (VIMSS) compare
b2786 barA +0.7 6.8 hybrid sensory histidine kinase, in two-component regulatory system with UvrY (NCBI) compare
b4041 plsB +0.7 2.3 glycerol-3-phosphate acyltransferase (VIMSS) compare
b0728 sucC +0.7 4.3 succinyl-CoA synthetase subunit beta (NCBI) compare
b2741 rpoS +0.7 4.4 RNA polymerase sigma factor (NCBI) compare
b1506 yneL +0.7 1.3 predicted transcriptional regulator (NCBI) compare
b4065 yjcE +0.7 5.7 predicted cation/proton antiporter (NCBI) compare
b1708 nlpC +0.7 2.7 predicted lipoprotein (NCBI) compare
b4520 ymgF +0.7 1.3 hypothetical protein (NCBI) compare
b1049 mdoH +0.7 4.9 glucosyltransferase MdoH (NCBI) compare
b1618 uidR +0.7 3.5 DNA-binding transcriptional repressor (NCBI) compare
b1307 pspD +0.7 1.9 peripheral inner membrane phage-shock protein (NCBI) compare
b4503 yafF +0.6 0.6 no description compare
b2260 menE +0.6 5.0 O-succinylbenzoic acid--CoA ligase (NCBI) compare
b1063 yceB +0.6 2.5 predicted lipoprotein (NCBI) compare
b0729 sucD +0.6 1.9 succinyl-CoA synthetase subunit alpha (NCBI) compare
b1233 ychJ +0.6 3.6 hypothetical protein (NCBI) compare
b0145 dksA +0.6 2.3 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b4426 isrA +0.6 1.4 unknown RNA (NCBI) compare
b4702 mgtL +0.6 1.4 regulatory leader peptide for mgtA (NCBI) compare
b1724 ydiZ +0.6 2.2 hypothetical protein (NCBI) compare
b0059 hepA +0.6 5.4 ATP-dependent helicase HepA (NCBI) compare
b0912 ihfB +0.6 1.8 integration host factor subunit beta (NCBI) compare
b1601 ydgG +0.6 3.3 predicted inner membrane protein (NCBI) compare
b1853 yebK +0.6 1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b4431 rprA +0.6 1.8 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b3026 qseC +0.6 2.7 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b3198 kdsC +0.6 2.0 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b2625 yfjI +0.6 1.1 CP4-57 prophage; predicted protein (NCBI) compare
b1760 ynjH +0.6 2.0 hypothetical protein (NCBI) compare
b2264 menD +0.6 3.8 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b3962 udhA +0.5 3.7 putative oxidoreductase (VIMSS) compare
b4402 yjjY +0.5 0.6 hypothetical protein (NCBI) compare
b1189 dadA +0.5 3.6 D-amino acid dehydrogenase (quinone) DadA (EC 1.4.5.1) (from data) compare
b1217 chaB +0.5 0.6 cation transport regulator (NCBI) compare
b1190 dadX +0.5 2.5 alanine racemase (NCBI) compare
b1205 ychH +0.5 1.4 predicted inner membrane protein (NCBI) compare
b2642 yfjW +0.5 0.7 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b3950 frwB +0.5 2.3 predicted enzyme IIB component of PTS (NCBI) compare
b1263 trpD +0.5 1.7 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b1160 elbA +0.5 0.6 hypothetical protein (NCBI) compare
b0388 aroL +0.5 2.7 shikimate kinase II (NCBI) compare
b1446 ydcY +0.5 1.5 hypothetical protein (NCBI) compare
b0908 aroA +0.5 3.4 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b4480 hdfR +0.5 2.3 transcriptional regulator HdfR (NCBI) compare
b2362 yfdS +0.5 2.2 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b2444 yffM +0.5 1.0 CPZ-55 prophage; predicted protein (NCBI) compare
b0530 sfmA +0.5 1.8 putative fimbrial-like protein (VIMSS) compare
b2684 mprA +0.5 1.8 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b0739 tolA +0.5 1.9 cell envelope integrity inner membrane protein TolA (NCBI) compare
b1946 fliN +0.5 1.5 flagellar motor switch protein (NCBI) compare
b1891 flhC +0.5 1.7 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b0613 citG +0.5 1.4 triphosphoribosyl-dephospho-CoA transferase (NCBI) compare
b0556 rzpD +0.5 1.9 DLP12 prophage; predicted murein endopeptidase (NCBI) compare
b1540 ydfH +0.5 0.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b0998 torD +0.5 0.9 chaperone protein TorD (NCBI) compare
b0720 gltA +0.5 2.9 citrate synthase (NCBI) compare
b2136 yohD +0.5 2.2 orf, hypothetical protein (VIMSS) compare
b4071 nrfB +0.4 2.2 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b3264 envR +0.4 2.0 DNA-binding transcriptional regulator (NCBI) compare
b2579 yfiD +0.4 1.3 pyruvate formate lyase subunit (NCBI) compare
b3636 rpmG +0.4 0.7 50S ribosomal protein L33 (NCBI) compare
b3930 menA +0.4 3.9 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b1347 ydaC +0.4 0.3 Rac prophage; predicted protein (NCBI) compare
b2003 yeeT +0.4 1.6 CP4-44 prophage; predicted protein (NCBI) compare
b3146 yraL +0.4 2.8 predicted methyltransferase (NCBI) compare
b0830 yliB +0.4 2.8 predicted peptide transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b1598 ydgD +0.4 1.7 predicted peptidase (NCBI) compare
b1823 cspC +0.4 0.8 stress protein, member of the CspA-family (NCBI) compare
b2258 b2258 +0.4 0.8 putative transport/receptor protein (VIMSS) compare
b1671 ydhX +0.4 1.7 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b2493 yfgO +0.4 2.7 predicted inner membrane protein (NCBI) compare
b3099 yqjE +0.4 2.9 conserved inner membrane protein (NCBI) compare
b1411 ynbD +0.4 2.4 predicted phosphatase, inner membrane protein (NCBI) compare
b0113 pdhR +0.4 2.7 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b2539 hcaF +0.4 2.4 3-phenylpropionate dioxygenase, small (beta) subunit (NCBI) compare
b2248 yfaX +0.4 0.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b3611 yibN +0.4 2.0 predicted rhodanese-related sulfurtransferase (NCBI) compare
b4181 yjfI +0.4 1.7 hypothetical protein (NCBI) compare
b1904 yecR +0.4 1.9 hypothetical protein (NCBI) compare
b2217 rcsB +0.4 2.6 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b2744 surE +0.4 2.7 acid phosphatase (NCBI) compare
b1679 sufE +0.4 1.7 cysteine desufuration protein SufE (NCBI) compare
b0440 hupB +0.4 2.6 HU, DNA-binding transcriptional regulator, beta subunit (NCBI) compare
b4408 csrB +0.4 1.0 CsrA-binding sRNA, antagonizing CsrA regulation (NCBI) compare
b1520 b1520 +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b3785 wzzE +0.4 3.1 putative transport protein (VIMSS) compare
b4172 hfq +0.4 0.4 RNA-binding protein Hfq (NCBI) compare
b1171 b1171 +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b1572 ydfB +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b0866 ybjQ +0.4 0.8 hypothetical protein (NCBI) compare
b3623 rfaK +0.4 0.9 lipopolysaccharide core biosynthesis (NCBI) compare
b3098 yqjD +0.4 1.7 hypothetical protein (NCBI) compare
b4651 yibW +0.4 0.2 no description compare
b2156 lysP +0.4 2.9 lysine transporter (NCBI) compare
b0685 ybfE +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b2261 menC +0.4 2.5 O-succinylbenzoate synthase (NCBI) compare
b1429 tehA +0.4 0.6 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b1392 paaE +0.4 2.3 predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI) compare
b3032 cpdA +0.4 2.6 cyclic 3',5'-adenosine monophosphate phosphodiesterase (NCBI) compare
b3764 yifE +0.4 2.2 hypothetical protein (NCBI) compare
b0951 pqiB +0.4 2.8 paraquat-inducible protein B (NCBI) compare
b0379 yaiY +0.4 1.5 predicted inner membrane protein (NCBI) compare
b1164 ycgZ +0.4 0.7 hypothetical protein (NCBI) compare
b4450 arcZ +0.4 1.2 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b3100 yqjK +0.4 1.5 hypothetical protein (NCBI) compare
b1346 ydaQ +0.4 0.6 orf, hypothetical protein (VIMSS) compare
b0722 sdhD +0.4 1.8 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b4529 ydbJ +0.4 0.5 hypothetical protein (NCBI) compare
b2700 ygaD +0.4 1.3 competence damage-inducible protein A (NCBI) compare
b1357 ydaS +0.4 1.0 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b2794 yqcD +0.4 2.4 hypothetical protein (NCBI) compare
b0018 mokC +0.4 0.8 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b0696 kdpC +0.4 1.0 potassium-transporting ATPase subunit C (NCBI) compare
b2749 ygbE +0.4 2.1 conserved inner membrane protein (NCBI) compare
b4260 pepA +0.4 2.9 leucyl aminopeptidase (NCBI) compare
b3271 yhdZ +0.4 2.1 predicted amino-acid transporter subunit (NCBI) compare
b2182 bcr +0.4 1.9 bicyclomycin/multidrug efflux system (NCBI) compare
b1251 yciI +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b4280 yjhC +0.3 2.4 putative dehydrogenase (VIMSS) compare
b1134 ymfB +0.3 1.0 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b0210 yafE +0.3 1.3 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b2468 aegA +0.3 2.4 fused predicted oxidoreductase: FeS binding subunit/NAD/FAD-binding subunit (NCBI) compare
b1712 ihfA +0.3 0.8 integration host factor subunit alpha (NCBI) compare
b2322 yfcJ +0.3 2.2 predicted transporter (NCBI) compare
b3194 yrbE +0.3 2.7 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0310 ykgH +0.3 0.5 predicted inner membrane protein (NCBI) compare
b1865 nudB +0.3 0.8 dATP pyrophosphohydrolase (NCBI) compare
b1607 ydgC +0.3 0.6 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b1626 b1626 +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b3243 aaeR +0.3 1.8 predicted DNA-binding transcriptional regulator, efflux system (NCBI) compare
b1945 fliM +0.3 1.9 flagellar motor switch protein M (NCBI) compare
b2169 fruB +0.3 2.5 fused fructose-specific PTS enzymes: IIA component/HPr component (NCBI) compare
b3928 yiiU +0.3 1.9 hypothetical protein (NCBI) compare
b1644 b1644 +0.3 1.4 putative membrane protein (VIMSS) compare
b2477 nlpB +0.3 1.4 lipoprotein-34 (VIMSS) compare
b2750 cysC +0.3 2.0 adenylylsulfate kinase (NCBI) compare
b4111 proP +0.3 2.7 proline/glycine betaine transporter (NCBI) compare
b2236 yfaE +0.3 1.6 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b4246 pyrL +0.3 0.8 pyrBI operon leader peptide (NCBI) compare
b1622 malY +0.3 1.6 bifunctional beta-cystathionase, PLP-dependent/ regulator of maltose regulon (NCBI) compare
b1172 ymgG +0.3 2.0 conserved protein, UPF0757 family (RefSeq) compare
b0125 hpt +0.3 1.6 hypoxanthine phosphoribosyltransferase (VIMSS) compare
b4698 mgrR +0.3 1.3 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b1836 yebV +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b1661 cfa +0.3 2.2 cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI) compare
b1639 ydhA +0.3 2.0 orf, hypothetical protein (VIMSS) compare
b2189 proL +0.3 0.8 tRNA-Pro (NCBI) compare
b1804 rnd +0.3 1.8 ribonuclease D (NCBI) compare
b2457 cchA +0.3 1.5 detox protein (VIMSS) compare
b1802 yeaW +0.3 1.5 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b3563 yiaB +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b1082 flgK +0.3 1.6 flagellar hook-associated protein K (NCBI) compare
b3462 ftsX +0.3 1.6 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b4175 hflC +0.3 2.5 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b3153 yhbO +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b2808 gcvA +0.3 1.4 DNA-binding transcriptional dual regulator (NCBI) compare
b2366 dsdA +0.3 1.8 D-serine dehydratase (NCBI) compare
b4704 arrS +0.3 0.1 Antisense sRNA ArrS, function unknown (NCBI) compare
b0549 ybcO +0.3 1.2 DLP12 prophage; predicted protein (NCBI) compare
b2500 purN +0.3 1.1 phosphoribosylglycinamide formyltransferase (NCBI) compare
b1187 fadR +0.3 1.2 fatty acid metabolism regulator (NCBI) compare
b1244 oppB +0.3 1.9 oligopeptide permease ABC transporter membrane protein (NCBI) compare


Specific Phenotypes

For 2 genes in this experiment

For stress Isobutyl alcohol in Escherichia coli BW25113

For stress Isobutyl alcohol across organisms