Experiment set17IT031 for Pseudomonas putida KT2440
M9 minimal media_noCarbon with D-Glucose 10 mM; 15C
Group: carbon sourceMedia: M9 minimal media_noCarbon + D-Glucose (10 mM)
Culturing: Putida_ML5_PNNL, tube, Aerobic, at 15 (C)
By: Rob Egbert on 11/7/19
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride
Specific Phenotypes
For 2 genes in this experiment
For carbon source D-Glucose in Pseudomonas putida KT2440
For carbon source D-Glucose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Alginate metabolism | 1 |
| Mannose Metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-mannose degradation I | 2 | 1 | 1 |
| D-mannose degradation II | 2 | 1 | 1 |
| β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation | 3 | 2 | 1 |
| mannitol biosynthesis | 3 | 1 | 1 |
| GDP-mannose biosynthesis | 4 | 4 | 1 |
| mannitol degradation II | 4 | 2 | 1 |
| 1,5-anhydrofructose degradation | 5 | 2 | 1 |
| β-(1,4)-mannan degradation | 7 | 2 | 1 |
| colanic acid building blocks biosynthesis | 11 | 9 | 1 |
| superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis | 14 | 7 | 1 |