Experiment set16S226 for Agrobacterium fabrum C58
AgroPhage_C10
Group: phageMedia: LB_plus_SM_buffer + Kan (50 ug/ml)
Culturing: Agro_ML11a, 48 well microplate; Epoch2, Aerobic, at 30 (C), shaken=double orbital, continuous, 205cpm
By: James on 2/20/23
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 13 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Terminal cytochrome C oxidases | 4 |
| Biogenesis of cbb3-type cytochrome c oxidases | 2 |
| Biotin biosynthesis | 1 |
| ECF class transporters | 1 |
| Experimental tye | 1 |
| Heme and Siroheme Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| arsenite to oxygen electron transfer | 2 | 1 | 1 |
| arsenite to oxygen electron transfer (via azurin) | 3 | 1 | 1 |
| alginate degradation | 7 | 4 | 2 |
| aerobic respiration I (cytochrome c) | 4 | 3 | 1 |
| heme b biosynthesis II (oxygen-independent) | 4 | 3 | 1 |
| aerobic respiration II (cytochrome c) (yeast) | 4 | 2 | 1 |
| superpathway of heme b biosynthesis from uroporphyrinogen-III | 6 | 4 | 1 |
| Fe(II) oxidation | 6 | 2 | 1 |
| 3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic) | 9 | 2 | 1 |
| superpathway of bacteriochlorophyll a biosynthesis | 26 | 6 | 1 |