Experiment set16S220 for Agrobacterium fabrum C58

AgroPhage_C04

Group: phage
Media: LB_plus_SM_buffer + Kan (50 ug/ml)
Culturing: Agro_ML11a, 48 well microplate; Epoch2, Aerobic, at 30 (C), shaken=double orbital, continuous, 205cpm
By: James on 2/20/23
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 13 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Terminal cytochrome C oxidases	4
Biogenesis of cbb3-type cytochrome c oxidases	2
Experimental tye	1
Heme and Siroheme Biosynthesis	1
Transcription factors bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Oxidative phosphorylation
• Porphyrin and chlorophyll metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
arsenite to oxygen electron transfer	2	1	1
arsenite to oxygen electron transfer (via azurin)	3	1	1
aerobic respiration I (cytochrome c)	4	3	1
heme b biosynthesis II (oxygen-independent)	4	3	1
aerobic respiration II (cytochrome c) (yeast)	4	2	1
superpathway of heme b biosynthesis from uroporphyrinogen-III	6	4	1
Fe(II) oxidation	6	2	1
3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic)	9	2	1
superpathway of bacteriochlorophyll a biosynthesis	26	6	1