Experiment set15IT068 for Pseudomonas putida KT2440

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Butyl stearate carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Butyl stearate (10 mM)
Culturing: Putida_ML5_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
By: Mitchell Thompson on 10/22/19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 8 genes in this experiment

For carbon source Butyl stearate in Pseudomonas putida KT2440

For carbon source Butyl stearate across organisms

SEED Subsystems

Subsystem #Specific
Biotin biosynthesis 1
n-Phenylalkanoic acid degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
long-chain fatty acid activation 1 1 1
γ-linolenate biosynthesis II (animals) 2 1 1
linoleate biosynthesis II (animals) 2 1 1
3-methyl-branched fatty acid α-oxidation 6 3 2
oleate biosynthesis I (plants) 3 1 1
alkane biosynthesis II 3 1 1
phytol degradation 4 3 1
phosphatidylcholine acyl editing 4 1 1
wax esters biosynthesis II 4 1 1
long chain fatty acid ester synthesis (engineered) 4 1 1
sporopollenin precursors biosynthesis 18 4 4
octane oxidation 5 4 1
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
fatty acid salvage 6 6 1
stearate biosynthesis II (bacteria and plants) 6 5 1
stearate biosynthesis IV 6 4 1
6-gingerol analog biosynthesis (engineered) 6 3 1
stearate biosynthesis I (animals) 6 1 1
capsaicin biosynthesis 7 3 1
ceramide degradation by α-oxidation 7 2 1
icosapentaenoate biosynthesis III (8-desaturase, mammals) 7 1 1
arachidonate biosynthesis III (6-desaturase, mammals) 7 1 1
icosapentaenoate biosynthesis II (6-desaturase, mammals) 7 1 1
2-deoxy-D-ribose degradation II 8 4 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
suberin monomers biosynthesis 20 4 2
superpathway of fatty acid biosynthesis II (plant) 43 38 4
palmitate biosynthesis II (type II fatty acid synthase) 31 29 2
cutin biosynthesis 16 1 1
superpathway of fatty acids biosynthesis (E. coli) 53 51 2
palmitate biosynthesis III 29 28 1
oleate β-oxidation 35 30 1