Experiment set14IT066 for Escherichia coli BW25113

Compare to:

LB with 2-nitrobenzenesulfonyl fluoride

200 most detrimental genes:

  gene name fitness t score description  
b0851 nfsA +6.3 22.0 nitroreductase A, NADPH-dependent, FMN-dependent (NCBI) compare
b1531 marA +4.4 7.7 multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS) compare
b0464 acrR +3.5 24.1 DNA-binding transcriptional repressor (NCBI) compare
b0912 ihfB +3.1 9.8 integration host factor subunit beta (NCBI) compare
b1217 chaB +3.0 2.0 cation transport regulator (NCBI) compare
b2689 yqaA +3.0 4.3 conserved inner membrane protein (NCBI) compare
b0578 nfnB +2.7 15.8 dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive (NCBI) compare
b0850 ybjC +2.3 1.3 predicted inner membrane protein (NCBI) compare
b1530 marR +2.3 2.5 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b0852 rimK +2.2 4.2 ribosomal protein S6 modification protein (NCBI) compare
b0606 ahpF +2.0 11.9 alkyl hydroperoxide reductase, F52a subunit; detoxification of hydroperoxides (VIMSS) compare
b2642 yfjW +1.9 1.2 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b0605 ahpC +1.8 4.5 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b1429 tehA +1.8 1.1 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b3979 thrT +1.8 1.0 tRNA-Thr (NCBI) compare
b4402 yjjY +1.8 1.9 hypothetical protein (NCBI) compare
b0014 dnaK +1.6 2.5 molecular chaperone DnaK (NCBI) compare
b3405 ompR +1.6 6.8 osmolarity response regulator (NCBI) compare
b1160 elbA +1.6 1.4 hypothetical protein (NCBI) compare
b2444 yffM +1.6 2.3 CPZ-55 prophage; predicted protein (NCBI) compare
b0059 hepA +1.5 12.0 ATP-dependent helicase HepA (NCBI) compare
b0247 ykfG +1.5 2.0 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b4313 fimE +1.5 9.2 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b2215 ompC +1.4 8.7 outer membrane porin protein C (NCBI) compare
b4407 thiS +1.3 2.1 sulfur carrier protein ThiS (NCBI) compare
b2480 bcp +1.3 5.3 thioredoxin-dependent thiol peroxidase (NCBI) compare
b1013 ycdC +1.3 2.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b1553 ydfP +1.3 2.1 Qin prophage; conserved protein (NCBI) compare
b0623 cspE +1.3 2.5 cold shock protein E (NCBI) compare
b3404 envZ +1.3 8.0 osmolarity sensor protein (NCBI) compare
b3780 rhlB +1.2 9.1 ATP-dependent RNA helicase (NCBI) compare
b3812 yigB +1.2 5.9 predicted hydrolase (NCBI) compare
b4416 rybA +1.2 1.3 unknown RNA (NCBI) compare
b1946 fliN +1.2 3.0 flagellar motor switch protein (NCBI) compare
b1831 proQ +1.2 2.5 putative solute/DNA competence effector (NCBI) compare
b2421 cysM +1.2 3.8 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b0927 ycbL +1.2 2.8 predicted metal-binding enzyme (NCBI) compare
b2804 fucU +1.2 2.1 L-fucose mutarotase (NCBI) compare
b2029 gnd +1.1 6.1 6-phosphogluconate dehydrogenase (NCBI) compare
b1656 sodB +1.1 1.9 superoxide dismutase, Fe (NCBI) compare
b0127 yadG +1.1 5.0 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b1138 ymfE +1.1 2.0 e14 prophage; predicted inner membrane protein (NCBI) compare
b2783 chpR +1.1 2.2 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b3493 pitA +1.1 6.1 phosphate transporter, low-affinity (NCBI) compare
b2582 trxC +1.1 2.3 thioredoxin 2 (NCBI) compare
b1882 cheY +1.1 0.6 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b2392 mntH +1.0 6.0 manganese transport protein MntH (NCBI) compare
b1153 ymfQ +1.0 3.2 e14 prophage; conserved protein (NCBI) compare
b2209 eco +1.0 1.7 ecotin precursor (NCBI) compare
b2446 yffO +1.0 1.8 CPZ-55 prophage; predicted protein (NCBI) compare
b1778 yeaA +1.0 1.8 methionine sulfoxide reductase B (NCBI) compare
b4461 yfjD +1.0 5.7 predicted inner membrane protein (RefSeq) compare
b4699 fnrS +1.0 0.9 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1907 tyrP +1.0 3.4 tyrosine transporter (NCBI) compare
b0888 trxB +1.0 2.6 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b1818 manY +0.9 1.2 mannose-specific enzyme IIC component of PTS (NCBI) compare
b1824 yobF +0.9 2.0 hypothetical protein (NCBI) compare
b1567 intK +0.9 1.2 Qin prophage; predicted protein (RefSeq) compare
b4041 plsB +0.9 1.9 glycerol-3-phosphate acyltransferase (VIMSS) compare
b1558 cspF +0.9 1.1 Qin prophage; cold shock protein (NCBI) compare
b1607 ydgC +0.9 1.1 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b4655 ythA +0.9 0.9 expressed protein (RefSeq) compare
b1332 ynaJ +0.9 0.6 predicted inner membrane protein (NCBI) compare
b1524 yneH +0.9 1.0 predicted glutaminase (NCBI) compare
b2377 yfdY +0.9 1.7 predicted inner membrane protein (NCBI) compare
b4589 ylcI +0.9 1.5 hypothetical protein (RefSeq) compare
b2635 ypjK +0.9 2.2 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b1253 yciA +0.9 2.5 predicted hydrolase (NCBI) compare
b1837 yebW +0.9 1.7 orf, hypothetical protein (VIMSS) compare
b2502 ppx +0.9 4.6 exopolyphosphatase (NCBI) compare
b2263 yfbB +0.9 2.1 predicted peptidase (NCBI) compare
b4396 rob +0.9 5.7 DNA-binding transcriptional activator (NCBI) compare
b2198 ccmD +0.9 1.8 cytochrome c biogenesis protein (NCBI) compare
b1148 ymfM +0.9 2.0 e14 prophage; predicted protein (NCBI) compare
b4515 ybgT +0.9 1.4 hypothetical protein (NCBI) compare
b1884 cheR +0.9 2.1 chemotaxis regulator, protein-glutamate methyltransferase (NCBI) compare
b3119 tdcR +0.9 1.8 threonine dehydratase operon activator protein (VIMSS) compare
b1764 selD +0.8 2.4 selenophosphate synthetase (NCBI) compare
b4520 ymgF +0.8 1.0 hypothetical protein (NCBI) compare
b0015 dnaJ +0.8 4.9 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b4521 ycgI +0.8 2.0 hypothetical protein (NCBI) compare
b1235 rssB +0.8 4.2 response regulator of RpoS (NCBI) compare
b1804 rnd +0.8 2.5 ribonuclease D (NCBI) compare
b1753 ynjA +0.8 1.6 hypothetical protein (NCBI) compare
b1157 stfE +0.8 3.3 no description compare
b3930 menA +0.8 6.7 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b1400 paaY +0.8 1.4 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b1242 ychE +0.8 2.2 predicted inner membrane protein (NCBI) compare
b0826 moeB +0.8 4.0 molybdopterin biosynthesis protein MoeB (NCBI) compare
b1312 ycjP +0.8 3.2 predicted sugar transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1150 ymfR +0.8 0.8 e14 prophage; predicted protein (NCBI) compare
b1823 cspC +0.8 1.2 stress protein, member of the CspA-family (NCBI) compare
b2683 ygaH +0.8 3.5 predicted inner membrane protein (NCBI) compare
b0937 ssuE +0.8 1.5 NAD(P)H-dependent FMN reductase (NCBI) compare
b4551 yheV +0.8 0.9 hypothetical protein (NCBI) compare
b1152 ymfP +0.8 2.7 orf, hypothetical protein (VIMSS) compare
b0128 yadH +0.8 4.5 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1141 ymfG +0.8 2.5 e14 prophage; predicted excisionase (NCBI) compare
b2398 yfeC +0.8 1.7 orf, hypothetical protein (VIMSS) compare
b1867 yecD +0.7 2.8 orf, hypothetical protein (VIMSS) compare
b2622 intA +0.7 3.7 CP4-57 prophage; integrase (NCBI) compare
b1446 ydcY +0.7 1.0 hypothetical protein (NCBI) compare
b0456 ybaA +0.7 2.1 hypothetical protein (NCBI) compare
b1900 araG +0.7 2.3 fused L-arabinose transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1222 narX +0.7 2.4 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b0641 rlpB +0.7 1.4 minor lipoprotein (NCBI) compare
b0783 moaC +0.7 2.8 molybdenum cofactor biosynthesis protein C (NCBI) compare
b1842 holE +0.7 0.8 DNA polymerase III, theta subunit (NCBI) compare
b0785 moaE +0.7 2.8 molybdopterin synthase, large subunit (NCBI) compare
b1538 dcp +0.7 2.3 dipeptidyl carboxypeptidase II (NCBI) compare
b4505 insX +0.7 1.0 predicted IS protein (RefSeq) compare
b1963 b1963 +0.7 1.7 orf, hypothetical protein (VIMSS) compare
b2913 serA +0.7 4.1 D-3-phosphoglycerate dehydrogenase (NCBI) compare
b2938 speA +0.7 4.2 arginine decarboxylase (NCBI) compare
b0763 modA +0.7 2.8 molybdate transporter subunit (NCBI) compare
b1569 dicC +0.7 1.6 Qin prophage; DNA-binding transcriptional regulator for DicB (NCBI) compare
b0473 htpG +0.7 4.8 heat shock protein 90 (NCBI) compare
b1184 umuC +0.7 3.0 DNA polymerase V subunit UmuC (NCBI) compare
b3293 yhdN +0.7 2.3 hypothetical protein (NCBI) compare
b2136 yohD +0.7 1.4 orf, hypothetical protein (VIMSS) compare
b1588 ynfF +0.7 1.6 oxidoreductase subunit (RefSeq) compare
b1441 ydcT +0.7 1.9 predicted spermidine/putrescine transporter subunit (NCBI) compare
b1287 yciW +0.7 1.7 putative oxidoreductase (VIMSS) compare
b4387 ytjB +0.7 2.6 hypothetical protein (NCBI) compare
b4693 aaaE +0.7 2.9 no description compare
b1348 lar +0.7 0.8 Rac prophage; restriction alleviation protein (NCBI) compare
b0550 rusA +0.6 1.3 DLP12 prophage; endonuclease RUS (NCBI) compare
b1482 osmC +0.6 0.8 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b1640 ydhH +0.6 1.8 anhydro-N-acetylmuramic acid kinase (NCBI) compare
b2592 clpB +0.6 4.7 protein disaggregation chaperone (NCBI) compare
b1158 pin +0.6 2.9 e14 prophage; site-specific DNA recombinase (NCBI) compare
b0729 sucD +0.6 1.5 succinyl-CoA synthetase subunit alpha (NCBI) compare
b2449 yffR +0.6 2.0 CPZ-55 prophage; predicted protein (NCBI) compare
b0761 modE +0.6 3.0 DNA-binding transcriptional dual regulator (NCBI) compare
b1940 fliH +0.6 2.6 flagellar biosynthesis; export of flagellar proteins? (VIMSS) compare
b1712 ihfA +0.6 1.0 integration host factor subunit alpha (NCBI) compare
b1313 ycjQ +0.6 2.7 predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI) compare
b1533 ydeD +0.6 1.4 orf, hypothetical protein (VIMSS) compare
b2094 gatA +0.6 2.4 galactitol-specific enzyme IIA component of PTS (NCBI) compare
b4552 yrhC +0.6 0.4 no description compare
b1434 ydcN +0.6 1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b3068 ygjF +0.6 1.7 G/U mismatch-specific DNA glycosylase (NCBI) compare
b4127 yjdJ +0.6 3.1 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b4675 yoaJ +0.6 1.6 expressed protein (RefSeq) compare
b2586 yfiM +0.6 1.6 predicted protein (RefSeq) compare
b4595 yciY +0.6 1.0 hypothetical protein (RefSeq) compare
b1591 b1591 +0.6 0.7 putative oxidoreductase component (VIMSS) compare
b1144 ymfJ +0.6 1.2 orf, hypothetical protein (VIMSS) compare
b1246 oppD +0.6 3.0 oligopeptide transporter ATP-binding component (NCBI) compare
b1154 ycfK +0.6 3.3 e14 prophage; predicted protein (NCBI) compare
b3427 yzgL +0.6 2.2 orf, hypothetical protein (VIMSS) compare
b2114 metG +0.6 1.0 methionyl-tRNA synthetase (NCBI) compare
b3206 npr +0.6 2.9 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1675 ydhZ +0.6 1.7 hypothetical protein (NCBI) compare
b4695 ykgT +0.6 0.7 no description compare
b4613 dinQ +0.6 1.0 Damage inducible, function unknown (RefSeq) compare
b4240 treB +0.6 4.2 fused trehalose(maltose)-specific PTS enzyme: IIB component/IIC component (NCBI) compare
b0782 moaB +0.6 1.0 molybdopterin biosynthesis protein B (NCBI) compare
b3166 truB +0.6 3.2 tRNA pseudouridine synthase B (NCBI) compare
b0167 glnD +0.6 1.5 PII uridylyl-transferase (NCBI) compare
b0289 yagV +0.6 1.8 orf, hypothetical protein (VIMSS) compare
b0644 ybeQ +0.6 3.3 orf, hypothetical protein (VIMSS) compare
b1064 grxB +0.6 2.2 glutaredoxin 2 (Grx2) (NCBI) compare
b0836 yliH +0.6 0.9 hypothetical protein (NCBI) compare
b0781 moaA +0.6 3.6 molybdenum cofactor biosynthesis protein A (NCBI) compare
b2183 rsuA +0.6 1.5 16S rRNA pseudouridylate 516 synthase (NCBI) compare
b3672 ivbL +0.6 1.8 ilvB operon leader peptide (NCBI) compare
b1419 ydcA +0.6 1.7 hypothetical protein (NCBI) compare
b3892 fdoI +0.6 3.2 formate dehydrogenase-O, cytochrome b556 subunit (NCBI) compare
b0108 ppdD +0.6 1.7 predicted major pilin subunit (NCBI) compare
b1744 astE +0.6 2.0 succinylglutamate desuccinylase (NCBI) compare
b1557 cspB +0.6 0.6 Qin prophage; cold shock protein (NCBI) compare
b3658 selC +0.6 1.3 tRNA-Sec (NCBI) compare
b1359 ydaU +0.6 1.4 Rac prophage; conserved protein (NCBI) compare
b3673 emrD +0.6 3.9 2-module integral membrane pump; multidrug resistance (VIMSS) compare
b1781 yeaE +0.5 2.0 predicted oxidoreductase (NCBI) compare
b2236 yfaE +0.5 1.1 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b4608 ryfC +0.5 1.5 Novel sRNA, function unknown (RefSeq) compare
b1120 cobB +0.5 1.7 deacetylase of acs and cheY, regulates chemotaxis (NCBI) compare
b3857 mobA +0.5 1.9 molybdopterin-guanine dinucleotide biosynthesis protein A (NCBI) compare
b2937 speB +0.5 2.5 agmatinase (NCBI) compare
b3366 nirD +0.5 2.0 nitrite reductase small subunit (NCBI) compare
b1251 yciI +0.5 1.2 orf, hypothetical protein (VIMSS) compare
b4000 hupA +0.5 2.0 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b3262 yhdJ +0.5 2.5 putative methyltransferase (VIMSS) compare
b2443 yffL +0.5 2.1 CPZ-55 prophage; predicted protein (NCBI) compare
b1134 ymfB +0.5 1.0 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b3644 yicC +0.5 2.6 hypothetical protein (NCBI) compare
b1511 ydeV +0.5 1.9 putative kinase (VIMSS) compare
b3002 yqhA +0.5 1.0 hypothetical protein (NCBI) compare
b1958 yedI +0.5 1.8 conserved inner membrane protein (NCBI) compare
b1733 chbG +0.5 1.7 hypothetical protein (NCBI) compare
b1070 flgN +0.5 0.7 export chaperone for FlgK and FlgL (NCBI) compare
b2953 yggU +0.5 0.8 orf, hypothetical protein (VIMSS) compare
b1248 b1248 +0.5 0.8 orf, hypothetical protein (VIMSS) compare
b0846 ybjK +0.5 1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b1260 trpA +0.5 1.8 tryptophan synthase subunit alpha (NCBI) compare
b1542 ydfI +0.5 1.0 predicted mannonate dehydrogenase (NCBI) compare
b4570 lomR +0.5 0.6 no description compare
b4701 sokX +0.5 0.3 sok-related sRNA, function unknown (RefSeq) compare


Specific Phenotypes

For 19 genes in this experiment

For stress 2-nitrobenzenesulfonyl fluoride in Escherichia coli BW25113

For stress 2-nitrobenzenesulfonyl fluoride across organisms