Experiment set14IT066 for Escherichia coli BW25113

LB with 2-nitrobenzenesulfonyl fluoride

Group: stress
Media: LB + 2-nitrobenzenesulfonyl fluoride (25 uM)
Culturing: Keio_ML9a, 24-well transparent microplate; Tecan, Aerobic, at 37 (C), shaken=200 rpm
By: Kelly on 10/07/16
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 19 genes in this experiment

For stress 2-nitrobenzenesulfonyl fluoride in Escherichia coli BW25113

For stress 2-nitrobenzenesulfonyl fluoride across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, UvrABC system	3
DNA repair, bacterial RecFOR pathway	3
DNA-replication	2
Thioredoxin-disulfide reductase	2
DNA repair, bacterial	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
Glycine and Serine Utilization	1
Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes	1
Protein degradation	1
Pterin biosynthesis	1
Pyruvate Alanine Serine Interconversions	1
Threonine anaerobic catabolism gene cluster	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• Puromycin biosynthesis
• Selenoamino acid metabolism
• Glutathione metabolism
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Lipopolysaccharide biosynthesis
• Ether lipid metabolism
• alpha-Linolenic acid metabolism
• Sphingolipid metabolism
• Trinitrotoluene degradation
• Thiamine metabolism
• Riboflavin metabolism
• Nicotinate and nicotinamide metabolism
• Folate biosynthesis
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
tetrahydropteridine recycling	2	2	1
L-phenylalanine degradation V	3	1	1
heme b biosynthesis II (oxygen-independent)	4	4	1
heme b biosynthesis I (aerobic)	4	4	1
heme b biosynthesis V (aerobic)	4	4	1
superpathway of heme b biosynthesis from uroporphyrinogen-III	6	6	1
toxoflavin biosynthesis	7	4	1
superpathway of heme b biosynthesis from glycine	8	7	1
flavin biosynthesis I (bacteria and plants)	9	9	1
flavin biosynthesis III (fungi)	9	8	1
superpathway of heme b biosynthesis from glutamate	10	10	1
flavin biosynthesis II (archaea)	10	6	1