Experiment set14IT020 for Escherichia coli BW25113

Compare to:

LB with .5% DMSO

200 most detrimental genes:

  gene name fitness t score description  
b2741 rpoS +4.4 20.6 RNA polymerase sigma factor (NCBI) compare
b1831 proQ +3.9 7.1 putative solute/DNA competence effector (NCBI) compare
b1231 tyrT +1.6 0.9 tRNA-Tyr (NCBI) compare
b1882 cheY +1.6 0.9 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b4701 sokX +1.6 1.0 sok-related sRNA, function unknown (RefSeq) compare
b3133 agaV +1.4 1.4 PTS system, cytoplasmic, N-acetylgalactosamine-specific IIB component 2 (EIIB-AGA) (VIMSS) compare
b2444 yffM +1.4 2.0 CPZ-55 prophage; predicted protein (NCBI) compare
b2579 yfiD +1.2 2.3 pyruvate formate lyase subunit (NCBI) compare
b0503 ybbB +1.1 1.5 tRNA 2-selenouridine synthase, selenophosphate-dependent (NCBI) compare
b1103 ycfF +1.1 1.3 orf, hypothetical protein (VIMSS) compare
b0142 folK +1.0 0.6 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b2229 b2229 +1.0 1.4 orf, hypothetical protein (VIMSS) compare
b4416 rybA +1.0 1.1 unknown RNA (NCBI) compare
b1607 ydgC +1.0 1.2 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b2804 fucU +1.0 1.7 L-fucose mutarotase (NCBI) compare
b2792 yqcC +0.9 2.1 hypothetical protein (NCBI) compare
b1778 yeaA +0.9 1.6 methionine sulfoxide reductase B (NCBI) compare
b3672 ivbL +0.9 2.0 ilvB operon leader peptide (NCBI) compare
b0468 ybaN +0.9 1.6 conserved inner membrane protein (NCBI) compare
b4552 yrhC +0.9 0.6 no description compare
b1644 b1644 +0.9 2.3 putative membrane protein (VIMSS) compare
b0613 citG +0.9 1.6 triphosphoribosyl-dephospho-CoA transferase (NCBI) compare
b2643 yfjX +0.9 1.4 CP4-57 prophage; predicted antirestriction protein (NCBI) compare
b2586 yfiM +0.9 2.3 predicted protein (RefSeq) compare
b1446 ydcY +0.9 1.2 hypothetical protein (NCBI) compare
b0641 rlpB +0.9 1.7 minor lipoprotein (NCBI) compare
b3042 b3042 +0.9 2.3 orf, hypothetical protein (VIMSS) compare
b4595 yciY +0.9 1.5 hypothetical protein (RefSeq) compare
b4646 yrdE +0.9 1.6 no description compare
b1946 fliN +0.9 2.5 flagellar motor switch protein (NCBI) compare
b1104 ycfL +0.9 1.4 hypothetical protein (NCBI) compare
b1953 b1953 +0.9 2.1 orf, hypothetical protein (VIMSS) compare
b4407 thiS +0.8 1.3 sulfur carrier protein ThiS (NCBI) compare
b3107 yhaL +0.8 1.2 orf, hypothetical protein (VIMSS) compare
b2142 yohK +0.8 1.7 predicted inner membrane protein (NCBI) compare
b4373 rimI +0.8 1.7 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b4071 nrfB +0.8 1.5 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b4551 yheV +0.8 1.2 hypothetical protein (NCBI) compare
b2300 yfcE +0.8 1.3 orf, hypothetical protein (VIMSS) compare
b1055 yceA +0.8 1.4 hypothetical protein (NCBI) compare
b1613 manA +0.8 0.8 mannose-6-phosphate isomerase (VIMSS) compare
b2644 yfjY +0.8 1.8 CP4-57 prophage; predicted DNA repair protein (NCBI) compare
b2209 eco +0.8 1.2 ecotin precursor (NCBI) compare
b1557 cspB +0.8 0.8 Qin prophage; cold shock protein (NCBI) compare
b1461 ydcE +0.8 1.2 4-oxalocrotonate tautomerase (NCBI) compare
b0840 deoR +0.8 2.3 DNA-binding transcriptional repressor (NCBI) compare
b2377 yfdY +0.8 1.4 predicted inner membrane protein (NCBI) compare
b0937 ssuE +0.8 1.3 NAD(P)H-dependent FMN reductase (NCBI) compare
b1618 uidR +0.8 2.1 DNA-binding transcriptional repressor (NCBI) compare
b0451 amtB +0.7 1.7 ammonium transporter (NCBI) compare
b0550 rusA +0.7 1.4 DLP12 prophage; endonuclease RUS (NCBI) compare
b2787 gudD +0.7 2.1 (D)-glucarate dehydratase 1 (NCBI) compare
b1677 lpp +0.7 1.0 murein lipoprotein (NCBI) compare
b1921 fliZ +0.7 1.6 orf, hypothetical protein (VIMSS) compare
b1709 btuD +0.7 0.6 vitamin B12-transporter ATPase (NCBI) compare
b0481 ybaK +0.7 2.5 hypothetical protein (NCBI) compare
b1743 spy +0.7 1.0 envelope stress induced periplasmic protein (NCBI) compare
b0357 yaiN +0.7 1.1 putative alpha helix chain (VIMSS) compare
b3882 yihU +0.7 1.8 predicted oxidoreductase with NAD(P)-binding Rossmann-fold domain (NCBI) compare
b3375 yhfR +0.7 4.4 putative transcriptional regulator (VIMSS) compare
b4589 ylcI +0.7 1.0 hypothetical protein (RefSeq) compare
b1741 ydjQ +0.7 2.0 endonuclease of nucleotide excision repair (NCBI) compare
b4545 ypdJ +0.7 0.9 no description compare
b1218 chaC +0.7 1.8 regulatory protein for cation transport (RefSeq) compare
b1072 flgA +0.7 1.8 flagellar basal body P-ring biosynthesis protein A (NCBI) compare
b1013 ycdC +0.7 1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b1480 sra +0.7 0.7 30S ribosomal subunit protein S22 (NCBI) compare
b2433 b2433 +0.6 1.1 orf, hypothetical protein (VIMSS) compare
b0247 ykfG +0.6 0.8 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b3990 thiH +0.6 1.9 thiamine biosynthesis protein ThiH (NCBI) compare
b4508 peaD +0.6 2.1 putative sensory transduction regulator (VIMSS) compare
b4521 ycgI +0.6 1.6 hypothetical protein (NCBI) compare
b2080 b2080 +0.6 1.2 orf, hypothetical protein (VIMSS) compare
b2944 sprT +0.6 1.5 hypothetical protein (NCBI) compare
b1727 yniC +0.6 0.9 predicted hydrolase (NCBI) compare
b1443 ydcV +0.6 2.0 predicted spermidine/putrescine transporter subunit (NCBI) compare
b1514 lsrC +0.6 0.9 AI2 transporter (NCBI) compare
b1867 yecD +0.6 2.2 orf, hypothetical protein (VIMSS) compare
b1307 pspD +0.6 1.0 peripheral inner membrane phage-shock protein (NCBI) compare
b2051 wcaH +0.6 0.9 GDP-mannose mannosyl hydrolase (VIMSS) compare
b4644 ypjI +0.6 1.9 no description compare
b0191 yaeJ +0.6 1.6 hypothetical protein (NCBI) compare
b1591 b1591 +0.6 0.6 putative oxidoreductase component (VIMSS) compare
b0505 allA +0.6 1.5 ureidoglycolate hydrolase (NCBI) compare
b1553 ydfP +0.6 0.9 Qin prophage; conserved protein (NCBI) compare
b2911 ssrS +0.6 0.6 6S regulatory RNA (NCBI) compare
b1837 yebW +0.6 1.0 orf, hypothetical protein (VIMSS) compare
b3605 lldD +0.6 2.8 L-lactate dehydrogenase, FMN-linked (NCBI) compare
b1465 narV +0.6 1.0 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b0729 sucD +0.6 1.4 succinyl-CoA synthetase subunit alpha (NCBI) compare
b1672 ydhW +0.6 1.9 hypothetical protein (NCBI) compare
b1260 trpA +0.6 2.1 tryptophan synthase subunit alpha (NCBI) compare
b1312 ycjP +0.6 2.3 predicted sugar transporter subunit: membrane component of ABC superfamily (NCBI) compare
b3208 mtgA +0.6 1.8 monofunctional biosynthetic peptidoglycan transglycosylase (NCBI) compare
b1394 paaG +0.6 1.4 enoyl-CoA hydratase (NCBI) compare
b1752 ydjZ +0.6 1.6 conserved inner membrane protein (NCBI) compare
b0785 moaE +0.6 2.2 molybdopterin synthase, large subunit (NCBI) compare
b3032 cpdA +0.6 2.4 cyclic 3',5'-adenosine monophosphate phosphodiesterase (NCBI) compare
b1184 umuC +0.6 2.5 DNA polymerase V subunit UmuC (NCBI) compare
b3753 rbsR +0.6 3.4 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b0841 ybjG +0.6 1.6 undecaprenyl pyrophosphate phosphatase (NCBI) compare
b2004 yeeU +0.6 1.4 CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b1844 exoX +0.6 1.9 exodeoxyribonuclease X (NCBI) compare
b3293 yhdN +0.6 1.8 hypothetical protein (NCBI) compare
b1542 ydfI +0.6 1.1 predicted mannonate dehydrogenase (NCBI) compare
b1656 sodB +0.6 1.0 superoxide dismutase, Fe (NCBI) compare
b1263 trpD +0.6 1.4 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b0556 rzpD +0.6 1.6 DLP12 prophage; predicted murein endopeptidase (NCBI) compare
b2317 dedA +0.6 1.3 conserved inner membrane protein (NCBI) compare
b2145 yeiS +0.5 1.5 predicted inner membrane protein (NCBI) compare
b1827 kdgR +0.5 1.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b1940 fliH +0.5 2.3 flagellar biosynthesis; export of flagellar proteins? (VIMSS) compare
b4608 ryfC +0.5 1.5 Novel sRNA, function unknown (RefSeq) compare
b2208 napF +0.5 1.9 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b1248 b1248 +0.5 1.0 orf, hypothetical protein (VIMSS) compare
b0852 rimK +0.5 1.6 ribosomal protein S6 modification protein (NCBI) compare
b0502 ylbG +0.5 0.9 no description compare
b1853 yebK +0.5 1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1378 ydbK +0.5 2.0 fused predicted pyruvate-flavodoxin oxidoreductase: conserved protein/conserved protein/FeS binding protein (NCBI) compare
b1651 gloA +0.5 1.2 glyoxalase I, Ni-dependent (NCBI) compare
b2531 iscR +0.5 0.6 DNA-binding transcriptional repressor (NCBI) compare
b0285 yagS +0.5 1.0 predicted oxidoreductase with FAD-binding domain (NCBI) compare
b4444 rygA +0.5 1.4 unknown RNA (NCBI) compare
b1221 narL +0.5 1.8 DNA-binding response regulator in two-component regulatory system with NarX (or NarQ) (NCBI) compare
b1140 intE +0.5 2.6 e14 prophage; predicted integrase (NCBI) compare
b1044 ymdA +0.5 1.8 hypothetical protein (NCBI) compare
b0999 cbpM +0.5 1.2 modulator of CbpA co-chaperone (NCBI) compare
b3718 yieK +0.5 1.6 hypothetical protein (NCBI) compare
b0717 ybgP +0.5 2.3 predicted assembly protein (NCBI) compare
b2398 yfeC +0.5 1.2 orf, hypothetical protein (VIMSS) compare
b1914 uvrY +0.5 1.5 response regulator (NCBI) compare
b2635 ypjK +0.5 1.2 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b0018 mokC +0.5 0.6 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b4695 ykgT +0.5 0.7 no description compare
b2182 bcr +0.5 1.5 bicyclomycin/multidrug efflux system (NCBI) compare
b1573 ydfC +0.5 0.6 Qin prophage; predicted protein (RefSeq) compare
b4387 ytjB +0.5 1.5 hypothetical protein (NCBI) compare
b4127 yjdJ +0.5 2.3 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b2439 eutL +0.5 1.5 predicted carboxysome structural protein with predicted role in ethanolamine utilization (NCBI) compare
b4438 cyaR +0.5 1.0 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b4592 yccB +0.5 0.9 hypothetical protein (RefSeq) compare
b0761 modE +0.5 2.2 DNA-binding transcriptional dual regulator (NCBI) compare
b4636 ybfI +0.5 1.9 no description compare
b1361 ydaW +0.5 0.3 orf, hypothetical protein (VIMSS) compare
b0994 torT +0.5 1.7 periplasmic sensory protein associated with the TorRS two-component regulatory system (NCBI) compare
b1370 insH-5 +0.5 0.5 Rac prophage; IS5 transposase and trans-activator (NCBI) compare
b2094 gatA +0.5 1.8 galactitol-specific enzyme IIA component of PTS (NCBI) compare
b1120 cobB +0.5 1.4 deacetylase of acs and cheY, regulates chemotaxis (NCBI) compare
b2202 napC +0.5 1.6 nitrate reductase, cytochrome c-type, periplasmic (NCBI) compare
b1795 yeaQ +0.5 1.5 conserved inner membrane protein (NCBI) compare
b1388 paaA +0.5 1.1 predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI) compare
b0765 modC +0.5 1.5 molybdate transporter subunit (NCBI) compare
b1180 ycgM +0.5 1.4 predicted isomerase/hydrolase (NCBI) compare
b2939 yqgB +0.5 0.7 predicted protein (RefSeq) compare
b1314 b1314 +0.5 1.3 putative transient receptor potential locus (VIMSS) compare
b2698 recX +0.5 1.0 RecA regulator RecX (NCBI) compare
b4370 leuQ +0.5 0.8 tRNA-Leu (NCBI) compare
b2123 yehR +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b4215 ytfI +0.4 0.4 hypothetical protein (NCBI) compare
b1602 pntB +0.4 1.5 pyridine nucleotide transhydrogenase (NCBI) compare
b1278 pgpB +0.4 0.7 phosphatidylglycerophosphatase B (NCBI) compare
b2783 chpR +0.4 0.8 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b1562 hokD +0.4 1.0 Qin prophage; small toxic polypeptide (NCBI) compare
b2262 menB +0.4 0.7 naphthoate synthase (NCBI) compare
b2506 yfgI +0.4 0.7 hypothetical protein (NCBI) compare
b1756 ynjD +0.4 1.7 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b2268 elaC +0.4 2.3 orf, hypothetical protein (VIMSS) compare
b1491 yddW +0.4 2.0 predicted liprotein (NCBI) compare
b0951 pqiB +0.4 2.2 paraquat-inducible protein B (NCBI) compare
b0707 ybgA +0.4 1.0 hypothetical protein (NCBI) compare
b0442 ybaV +0.4 1.5 hypothetical protein (NCBI) compare
b1635 gst +0.4 1.4 glutathionine S-transferase (NCBI) compare
b2728 hypC +0.4 1.5 protein required for maturation of hydrogenases 1 and 3 (NCBI) compare
b4509 ylcG +0.4 0.8 DLP12 prophage; predicted protein (NCBI) compare
b1253 yciA +0.4 1.1 predicted hydrolase (NCBI) compare
b0250 ykfB +0.4 1.3 CP4-6 prophage; predicted protein (NCBI) compare
b0784 moaD +0.4 1.1 molybdopterin synthase, small subunit (NCBI) compare
b3356 yhfA +0.4 1.8 hypothetical protein (NCBI) compare
b1025 ycdT +0.4 0.5 predicted diguanylate cyclase (NCBI) compare
b4493 gapC +0.4 0.9 no description compare
b2256 yfbH +0.4 2.0 hypothetical protein (NCBI) compare
b3351 kefG +0.4 1.6 glutathione-regulated potassium-efflux system ancillary protein (NCBI) compare
b3106 yhaK +0.4 1.1 predicted pirin-related protein (NCBI) compare
b2853 ygeI +0.4 0.2 hypothetical protein (NCBI) compare
b1793 yoaF +0.4 0.9 conserved outer membrane protein (NCBI) compare
b1839 yebY +0.4 0.6 hypothetical protein (NCBI) compare
b1796 yoaG +0.4 1.5 hypothetical protein (NCBI) compare
b4573 insZ +0.4 1.1 no description compare
b3188 sfsB +0.4 1.3 DNA-binding transcriptional activator of maltose metabolism (NCBI) compare
b1217 chaB +0.4 0.3 cation transport regulator (NCBI) compare
b2727 hypB +0.4 1.5 GTP hydrolase involved in nickel liganding into hydrogenases (NCBI) compare
b1819 manZ +0.4 1.1 mannose-specific enzyme IID component of PTS (NCBI) compare
b4002 yjaI +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b3119 tdcR +0.4 0.8 threonine dehydratase operon activator protein (VIMSS) compare
b3144 yraJ +0.4 2.5 predicted outer membrane protein (NCBI) compare
b4395 ytjC +0.4 1.9 phosphoglycerate mutase (NCBI) compare
b1639 ydhA +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b3437 gntK +0.4 1.7 gluconokinase 2, thermoresistant (VIMSS) compare
b0325 yahK +0.4 2.2 predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI) compare
b1622 malY +0.4 1.2 bifunctional beta-cystathionase, PLP-dependent/ regulator of maltose regulon (NCBI) compare


Specific Phenotypes

For 1 genes in this experiment

For stress Dimethyl Sulfoxide in Escherichia coli BW25113

For stress Dimethyl Sulfoxide across organisms