Experiment set13IT049 for Escherichia coli BW25113

LB with Nickel (II) chloride hexahydrate 1.5 mM

Group: stress
Media: LB + Nickel (II) chloride hexahydrate (1.5 mM)
Culturing: Keio_ML9a, 48 well microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 25-Jul-16
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: Plate1 D5,D6

Specific Phenotypes

For 7 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Escherichia coli BW25113

For stress Nickel (II) chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Cobalt-zinc-cadmium resistance	1
D-gluconate and ketogluconates metabolism	1
DNA-replication	1
Proline, 4-hydroxyproline uptake and utilization	1
Transcription factors bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Puromycin biosynthesis
• Glycine, serine and threonine metabolism
• Cysteine metabolism
• Nucleotide sugars metabolism
• Lipopolysaccharide biosynthesis
• alpha-Linolenic acid metabolism
• Trinitrotoluene degradation
• Folate biosynthesis
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-serine degradation	3	3	3
D-serine degradation	3	3	2
L-tryptophan degradation II (via pyruvate)	3	3	2
L-cysteine degradation II	3	2	2
glycine betaine degradation III	7	4	3
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis	5	2	2
glycine betaine degradation I	8	4	3
glycine degradation	3	3	1
L-methionine biosynthesis II	6	5	2
L-mimosine degradation	8	4	2
glutathione-mediated detoxification I	8	3	2
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	2
purine nucleobases degradation II (anaerobic)	24	17	3