Experiment set13IT037 for Escherichia coli BW25113

Compare to:

LB with Cisplatin 0.1 mg/ml

200 most detrimental genes:

  gene name fitness t score description  
b0958 sulA +3.8 16.2 SOS cell division inhibitor (NCBI) compare
b0912 ihfB +2.9 7.9 integration host factor subunit beta (NCBI) compare
b3493 pitA +2.8 19.3 phosphate transporter, low-affinity (NCBI) compare
b2518 ndk +2.7 6.0 nucleoside diphosphate kinase (NCBI) compare
b0092 ddlB +2.6 11.0 D-alanylalanine synthetase (NCBI) compare
b1235 rssB +2.3 12.6 response regulator of RpoS (NCBI) compare
b1349 recT +2.1 8.8 Rac prophage; recombination and repair protein (NCBI) compare
b3806 cyaA +2.0 8.1 adenylate cyclase (NCBI) compare
b1841 yobA +1.8 4.1 hypothetical protein (NCBI) compare
b4538 yoeF +1.8 2.8 hypothetical protein (RefSeq) compare
b2909 ygfB +1.7 7.4 orf, hypothetical protein (VIMSS) compare
b4042 dgkA +1.7 3.7 diacylglycerol kinase (NCBI) compare
b1840 yebZ +1.7 6.5 predicted inner membrane protein (NCBI) compare
b1540 ydfH +1.6 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b0059 hepA +1.6 10.4 ATP-dependent helicase HepA (NCBI) compare
b1712 ihfA +1.6 2.7 integration host factor subunit alpha (NCBI) compare
b1286 rnb +1.6 3.6 exoribonuclease II (NCBI) compare
b2516 yfgA +1.4 5.7 hypothetical protein (NCBI) compare
b0432 cyoA +1.4 9.2 cytochrome o ubiquinol oxidase subunit II (NCBI) compare
b3844 fre +1.4 6.4 NAD(P)H-flavin reductase (NCBI) compare
b1823 cspC +1.4 3.0 stress protein, member of the CspA-family (NCBI) compare
b2299 yfcD +1.4 6.8 predicted NUDIX hydrolase (NCBI) compare
b0428 cyoE +1.3 7.4 protoheme IX farnesyltransferase (NCBI) compare
b2281 nuoI +1.3 3.0 NADH dehydrogenase subunit I (NCBI) compare
b2218 rcsC +1.3 8.2 hybrid sensory kinase in two-component regulatory system with RcsB and YojN (RefSeq) compare
b2906 visC +1.3 5.9 hypothetical protein (NCBI) compare
b0632 dacA +1.3 5.6 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b1109 ndh +1.2 5.0 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b0429 cyoD +1.2 4.2 cytochrome o ubiquinol oxidase subunit IV (NCBI) compare
b3871 bipA +1.2 9.3 GTP-binding protein (NCBI) compare
b1246 oppD +1.2 5.5 oligopeptide transporter ATP-binding component (NCBI) compare
b3229 sspA +1.2 3.4 stringent starvation protein A (NCBI) compare
b3398 yrfF +1.2 3.2 predicted inner membrane protein (NCBI) compare
b4207 fklB +1.2 4.2 FKBP-type 22KD peptidyl-prolyl cis-trans isomerase (rotamase) (VIMSS) compare
b4596 yciZ +1.1 1.6 hypothetical protein (RefSeq) compare
b2479 gcvR +1.1 4.3 transcriptional regulation of gcv operon (VIMSS) compare
b2948 yqgE +1.1 5.0 orf, hypothetical protein (VIMSS) compare
b2284 nuoF +1.1 4.3 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b4234 yjgA +1.1 3.4 hypothetical protein (NCBI) compare
b1138 ymfE +1.1 1.7 e14 prophage; predicted inner membrane protein (NCBI) compare
b2502 ppx +1.1 7.1 exopolyphosphatase (NCBI) compare
b2216 rcsD +1.1 6.9 phosphotransfer intermediate protein in two-component regulatory system with RcsBC (NCBI) compare
b1247 oppF +1.1 4.5 oligopeptide transporter subunit (NCBI) compare
b1850 eda +1.1 2.0 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b0502 ylbG +1.1 2.9 no description compare
b1920 fliY +1.0 2.6 cystine transporter subunit (NCBI) compare
b2911 ssrS +1.0 1.1 6S regulatory RNA (NCBI) compare
b0081 mraZ +1.0 3.1 hypothetical protein (NCBI) compare
b3636 rpmG +1.0 1.5 50S ribosomal protein L33 (NCBI) compare
b2684 mprA +1.0 4.4 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b2283 nuoG +1.0 5.1 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b2279 nuoK +1.0 2.4 NADH dehydrogenase subunit K (NCBI) compare
b4701 sokX +1.0 0.6 sok-related sRNA, function unknown (RefSeq) compare
b4522 yciX_1 +1.0 2.2 hypothetical protein (NCBI) compare
b1103 ycfF +0.9 1.4 orf, hypothetical protein (VIMSS) compare
b1284 yciT +0.9 3.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b2287 nuoB +0.9 2.6 NADH dehydrogenase subunit B (NCBI) compare
b1244 oppB +0.9 4.0 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b0580 ybdJ +0.9 2.1 predicted inner membrane protein (NCBI) compare
b1465 narV +0.9 2.1 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b1242 ychE +0.9 2.4 predicted inner membrane protein (NCBI) compare
b2426 ucpA +0.9 2.1 putative oxidoreductase (VIMSS) compare
b0430 cyoC +0.9 3.2 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b3199 yrbK +0.9 1.9 hypothetical protein (NCBI) compare
b4511 ybdZ +0.9 1.8 hypothetical protein (NCBI) compare
b2277 nuoM +0.9 4.5 NADH dehydrogenase subunit M (NCBI) compare
b4066 yjcF +0.9 0.5 hypothetical protein (NCBI) compare
b1245 oppC +0.8 3.8 oligopeptide transporter subunit (NCBI) compare
b0226 dinJ +0.8 2.5 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b1104 ycfL +0.8 1.5 hypothetical protein (NCBI) compare
b1557 cspB +0.8 1.1 Qin prophage; cold shock protein (NCBI) compare
b2282 nuoH +0.8 2.7 NADH dehydrogenase subunit H (NCBI) compare
b1231 tyrT +0.8 0.7 tRNA-Tyr (NCBI) compare
b2285 nuoE +0.8 1.7 NADH dehydrogenase subunit E (NCBI) compare
b1162 ycgE +0.8 3.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b3071 yqjI +0.8 2.2 predicted transcriptional regulator (NCBI) compare
b3354 yheU +0.8 3.9 hypothetical protein (NCBI) compare
b4655 ythA +0.8 1.3 expressed protein (RefSeq) compare
b1317 ycjU +0.8 3.2 predicted beta-phosphoglucomutase (NCBI) compare
b0720 gltA +0.8 3.4 citrate synthase (NCBI) compare
b1243 oppA +0.8 2.6 oligopeptide transporter subunit (NCBI) compare
b0880 cspD +0.8 2.0 cold shock protein homolog (NCBI) compare
b0431 cyoB +0.8 5.3 cytochrome o ubiquinol oxidase subunit I (NCBI) compare
b0641 rlpB +0.8 2.0 minor lipoprotein (NCBI) compare
b1307 pspD +0.8 1.4 peripheral inner membrane phage-shock protein (NCBI) compare
b4068 yjcH +0.7 2.3 required for glycolate transport, with actP (from data) compare
b3184 yhbE +0.7 4.0 conserved inner membrane protein (NCBI) compare
b2022 hisB +0.7 2.7 imidazoleglycerolphosphate dehydratase and histidinol-phosphate phosphatase (VIMSS) compare
b0437 clpP +0.7 1.9 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b1279 yciS +0.7 0.7 conserved inner membrane protein (NCBI) compare
b4502 yeiW +0.7 2.0 hypothetical protein (NCBI) compare
b1608 rstA +0.7 2.5 DNA-binding response regulator in two-component regulatory system with RstB (RefSeq) compare
b1180 ycgM +0.7 2.3 predicted isomerase/hydrolase (NCBI) compare
b1633 nth +0.7 3.3 DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) (NCBI) compare
b1199 dhaL +0.7 3.1 dihydroxyacetone kinase, C-terminal domain (NCBI) compare
b1671 ydhX +0.7 1.1 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b0781 moaA +0.7 3.4 molybdenum cofactor biosynthesis protein A (NCBI) compare
b3183 obgE +0.7 1.0 GTPase involved in cell partioning and DNA repair (NCBI) compare
b3784 rfe +0.7 4.4 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b2450 yffS +0.7 0.9 CPZ-55 prophage; predicted protein (RefSeq) compare
b1936 intG +0.7 0.5 predicted defective phage integrase (pseudogene) (RefSeq) compare
b3973 birA +0.7 1.4 biotin--protein ligase (NCBI) compare
b2093 gatB +0.7 1.2 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b4408 csrB +0.7 1.9 CsrA-binding sRNA, antagonizing CsrA regulation (NCBI) compare
b1361 ydaW +0.7 0.2 orf, hypothetical protein (VIMSS) compare
b1891 flhC +0.7 2.5 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b1478 adhP +0.7 1.7 alcohol dehydrogenase (VIMSS) compare
b2419 yfeK +0.7 1.4 hypothetical protein (NCBI) compare
b4451 ryhB +0.7 1.1 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b2726 hypA +0.6 2.3 protein involved in nickel insertion into hydrogenases 3 (NCBI) compare
b2071 yegJ +0.6 1.3 hypothetical protein (NCBI) compare
b2672 ygaM +0.6 2.1 orf, hypothetical protein (VIMSS) compare
b0102 yacF +0.6 2.9 hypothetical protein (NCBI) compare
b0965 b0965 +0.6 1.3 orf, hypothetical protein (VIMSS) compare
b4606 ypfM +0.6 1.4 hypothetical protein (RefSeq) compare
b1926 fliT +0.6 1.7 predicted chaperone (NCBI) compare
b0998 torD +0.6 0.8 chaperone protein TorD (NCBI) compare
b4627 ykfL +0.6 1.8 no description compare
b0853 ybjN +0.6 2.8 predicted oxidoreductase (NCBI) compare
b1804 rnd +0.6 2.5 ribonuclease D (NCBI) compare
b1044 ymdA +0.6 2.9 hypothetical protein (NCBI) compare
b3834 yigP +0.6 2.7 hypothetical protein (NCBI) compare
b1273 yciN +0.6 1.2 hypothetical protein (NCBI) compare
b0934 ycbM +0.6 1.4 putative transport system permease protein (VIMSS) compare
b4180 rlmB +0.6 2.9 23S rRNA (Gm2251)-methyltransferase (NCBI) compare
b2446 yffO +0.6 1.4 CPZ-55 prophage; predicted protein (NCBI) compare
b1238 tdk +0.6 2.2 thymidine kinase (NCBI) compare
b1782 mipA +0.6 2.1 scaffolding protein for murein synthesizing machinery (NCBI) compare
b1073 flgB +0.6 1.6 flagellar basal-body rod protein B (NCBI) compare
b1883 cheB +0.6 1.7 chemotaxis-specific methylesterase (NCBI) compare
b2807 ygdD +0.6 1.9 conserved inner membrane protein (NCBI) compare
b0289 yagV +0.6 2.3 orf, hypothetical protein (VIMSS) compare
b2673 nrdH +0.6 1.3 glutaredoxin-like protein (NCBI) compare
b1348 lar +0.6 1.1 Rac prophage; restriction alleviation protein (NCBI) compare
b1456 rhsE +0.6 2.5 rhsE element core protein RshE (RefSeq) compare
b1379 hslJ +0.6 1.5 heat-inducible protein (NCBI) compare
b4653 yicT +0.6 1.6 no description compare
b3182 dacB +0.6 4.0 D-alanyl-D-alanine carboxypeptidase (NCBI) compare
b4210 ytfF +0.6 3.6 predicted inner membrane protein (RefSeq) compare
b4646 yrdE +0.6 1.4 no description compare
b3810 yigA +0.6 3.6 hypothetical protein (NCBI) compare
b4196 ulaD +0.6 2.1 3-keto-L-gulonate 6-phosphate decarboxylase (NCBI) compare
b1754 b1754 +0.6 1.2 orf, hypothetical protein (VIMSS) compare
b1438 ydcQ +0.6 1.2 predicted DNA-binding transcriptional regulator (RefSeq) compare
b2636 b2636 +0.5 2.4 orf, hypothetical protein (VIMSS) compare
b2377 yfdY +0.5 1.6 predicted inner membrane protein (NCBI) compare
b1939 fliG +0.5 1.4 flagellar motor switch protein G (NCBI) compare
b3210 arcB +0.5 2.7 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b2466 ypfG +0.5 2.2 hypothetical protein (NCBI) compare
b1426 ydcH +0.5 0.8 orf, hypothetical protein (VIMSS) compare
b4577 sgrS +0.5 1.1 small antisense RNA (NCBI) compare
b1214 ychA +0.5 2.5 predicted transcriptional regulator (NCBI) compare
b2708 gutQ +0.5 2.1 orf, hypothetical protein (VIMSS) compare
b4360 yjjA +0.5 1.5 putative glycoprotein/receptor (VIMSS) compare
b1948 fliP +0.5 2.0 flagellar biosynthesis protein P (NCBI) compare
b0494 tesA +0.5 2.9 multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1 (NCBI) compare
b2280 nuoJ +0.5 2.7 NADH dehydrogenase subunit J (NCBI) compare
b3658 selC +0.5 1.9 tRNA-Sec (NCBI) compare
b2955 yggW +0.5 2.8 coproporphyrinogen III oxidase (NCBI) compare
b4381 deoC +0.5 2.6 deoxyribose-phosphate aldolase (NCBI) compare
b4410 ecnA +0.5 0.9 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b0784 moaD +0.5 2.1 molybdopterin synthase, small subunit (NCBI) compare
b0550 rusA +0.5 1.3 DLP12 prophage; endonuclease RUS (NCBI) compare
b4075 nrfF +0.5 2.5 heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfF (NCBI) compare
b2555 yfhG +0.5 2.4 hypothetical protein (NCBI) compare
b0588 fepC +0.5 2.4 iron-enterobactin transporter subunit (NCBI) compare
b0765 modC +0.5 2.1 molybdate transporter subunit (NCBI) compare
b0775 bioB +0.5 1.9 biotin synthase (NCBI) compare
b3129 sohA +0.5 1.7 predicted regulator (NCBI) compare
b3920 yiiQ +0.5 2.5 hypothetical protein (NCBI) compare
b4246 pyrL +0.5 1.3 pyrBI operon leader peptide (NCBI) compare
b1418 cybB +0.5 1.5 cytochrome b(561) (VIMSS) compare
b2063 yegH +0.5 2.3 putative transport protein (VIMSS) compare
b0617 citD +0.5 1.1 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b4222 ytfP +0.5 2.3 hypothetical protein (NCBI) compare
b4532 yncN +0.5 1.3 orf, hypothetical protein (VIMSS) compare
b2823 ppdC +0.5 2.2 hypothetical protein (NCBI) compare
b2422 cysA +0.5 2.4 sulfate/thiosulfate transporter subunit (NCBI) compare
b1467 narY +0.5 2.1 nitrate reductase 2 (NRZ), beta subunit (NCBI) compare
b2258 b2258 +0.5 0.5 putative transport/receptor protein (VIMSS) compare
b0977 hyaF +0.5 1.6 protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI) compare
b2676 nrdF +0.5 2.2 ribonucleotide-diphosphate reductase beta subunit (NCBI) compare
b3352 yheS +0.5 3.7 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b2561 yfhH +0.5 3.0 orf, hypothetical protein (VIMSS) compare
b0590 fepD +0.5 3.0 iron-enterobactin transporter subunit (NCBI) compare
b1234 rssA +0.5 1.5 conserved protein (RefSeq) compare
b1562 hokD +0.5 1.2 Qin prophage; small toxic polypeptide (NCBI) compare
b1572 ydfB +0.5 0.8 orf, hypothetical protein (VIMSS) compare
b4699 fnrS +0.5 0.4 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b2278 nuoL +0.5 3.2 NADH dehydrogenase subunit L (NCBI) compare
b1159 mcrA +0.5 0.6 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b3740 gidB +0.5 3.1 glucose-inhibited division protein B (NCBI) compare
b1020 phoH +0.5 2.1 conserved protein with nucleoside triphosphate hydrolase domain (NCBI) compare
b3181 greA +0.5 1.8 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b2204 napH +0.5 1.7 quinol dehydrogenase membrane component (NCBI) compare
b1622 malY +0.5 1.8 bifunctional beta-cystathionase, PLP-dependent/ regulator of maltose regulon (NCBI) compare
b2771 ygcS +0.5 1.8 putative transport protein (VIMSS) compare
b3574 yiaJ +0.5 2.1 predicted DNA-binding transcriptional repressor (NCBI) compare
b2993 hybD +0.5 1.8 predicted maturation element for hydrogenase 2 (NCBI) compare
b1710 btuE +0.5 2.3 predicted glutathione peroxidase (NCBI) compare


Specific Phenotypes

For 31 genes in this experiment

For stress Cisplatin in Escherichia coli BW25113

For stress Cisplatin across organisms