Experiment set13IT037 for Escherichia coli BW25113

LB with Cisplatin 0.1 mg/ml

Group: stress
Media: LB + Cisplatin (0.1 mg/ml)
Culturing: Keio_ML9a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.0 generations
By: Adam on 25-Jul-16
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: Plate1 D5

Specific Phenotypes

For 32 genes in this experiment

For stress Cisplatin in Escherichia coli BW25113

For stress Cisplatin across organisms

SEED Subsystems

Subsystem	#Specific
DNA-replication	7
DNA repair, bacterial RecFOR pathway	5
DNA repair, UvrABC system	3
DNA repair, bacterial	3
DNA Repair Base Excision	2
ABC transporter oligopeptide (TC 3.A.1.5.1)	1
Arginine Deiminase Pathway	1
Arginine and Ornithine Degradation	1
Copper homeostasis	1
Folate Biosynthesis	1
Fructose utilization	1
Listeria phi-A118-like prophages	1
Nudix proteins (nucleoside triphosphate hydrolases)	1
Pentose phosphate pathway	1
Polyamine Metabolism	1
Transcription factors bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Biosynthesis of alkaloids derived from shikimate pathway
• Pentose phosphate pathway
• Ubiquinone and menaquinone biosynthesis
• Pyrimidine metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Lipopolysaccharide biosynthesis
• Naphthalene and anthracene degradation
• Folate biosynthesis
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pentose phosphate pathway (non-oxidative branch) I	5	5	1
pentose phosphate pathway	8	8	1
formaldehyde assimilation II (assimilatory RuMP Cycle)	9	7	1
Rubisco shunt	10	9	1
formaldehyde assimilation III (dihydroxyacetone cycle)	12	10	1
Bifidobacterium shunt	15	13	1
superpathway of glucose and xylose degradation	17	17	1