Experiment set13IT031 for Escherichia coli BW25113
LB with Nickel (II) chloride hexahydrate 1.5 mM
Group: stressMedia: LB + Nickel (II) chloride hexahydrate (1.5 mM)
Culturing: Keio_ML9a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.1 generations
By: Adam on 25-Jul-16
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: Plate1 C3
Specific Phenotypes
For 8 genes in this experiment
For stress Nickel (II) chloride hexahydrate in Escherichia coli BW25113
For stress Nickel (II) chloride hexahydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Cobalt-zinc-cadmium resistance | 1 |
| DNA-replication | 1 |
| Proline, 4-hydroxyproline uptake and utilization | 1 |
| Transcription factors bacterial | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Cysteine metabolism
- Purine metabolism
- Puromycin biosynthesis
- Glycine, serine and threonine metabolism
- Nucleotide sugars metabolism
- Lipopolysaccharide biosynthesis
- alpha-Linolenic acid metabolism
- Trinitrotoluene degradation
- Folate biosynthesis
- Porphyrin and chlorophyll metabolism
- Carotenoid biosynthesis - General
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-serine degradation | 3 | 3 | 3 |
| thiosulfate disproportionation IV (rhodanese) | 1 | 1 | 1 |
| cis-cyclopropane fatty acid (CFA) biosynthesis | 1 | 1 | 1 |
| D-serine degradation | 3 | 3 | 2 |
| L-tryptophan degradation II (via pyruvate) | 3 | 3 | 2 |
| L-cysteine degradation II | 3 | 2 | 2 |
| L-cysteine degradation III | 2 | 2 | 1 |
| sterculate biosynthesis | 2 | 1 | 1 |
| glycine betaine degradation III | 7 | 4 | 3 |
| felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis | 5 | 2 | 2 |
| glycine betaine degradation I | 8 | 4 | 3 |
| glycine degradation | 3 | 3 | 1 |
| L-methionine biosynthesis II | 6 | 5 | 2 |
| L-mimosine degradation | 8 | 4 | 2 |
| glutathione-mediated detoxification I | 8 | 3 | 2 |
| sulfide oxidation IV (mitochondria) | 5 | 2 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis II | 15 | 13 | 2 |
| purine nucleobases degradation II (anaerobic) | 24 | 17 | 3 |
| mycolate biosynthesis | 205 | 25 | 3 |
| superpathway of mycolate biosynthesis | 239 | 26 | 3 |