Experiment set13IT031 for Escherichia coli BW25113
LB with Nickel (II) chloride hexahydrate 1.5 mM
Group: stressMedia: LB + Nickel (II) chloride hexahydrate (1.5 mM)
Culturing: Keio_ML9a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.1 generations
By: Adam on 25-Jul-16
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: Plate1 C3
Specific Phenotypes
For 10 genes in this experiment
For stress Nickel (II) chloride hexahydrate in Escherichia coli BW25113
For stress Nickel (II) chloride hexahydrate across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Cysteine metabolism
- Purine metabolism
- Puromycin biosynthesis
- Glycine, serine and threonine metabolism
- Nucleotide sugars metabolism
- Lipopolysaccharide biosynthesis
- alpha-Linolenic acid metabolism
- Trinitrotoluene degradation
- Folate biosynthesis
- Porphyrin and chlorophyll metabolism
- Carotenoid biosynthesis - General
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-serine degradation | 3 | 3 | 3 |
| thiosulfate disproportionation IV (rhodanese) | 1 | 1 | 1 |
| cis-cyclopropane fatty acid (CFA) biosynthesis | 1 | 1 | 1 |
| L-tryptophan degradation II (via pyruvate) | 3 | 3 | 2 |
| D-serine degradation | 3 | 3 | 2 |
| L-cysteine degradation II | 3 | 2 | 2 |
| L-cysteine degradation III | 2 | 2 | 1 |
| sterculate biosynthesis | 2 | 1 | 1 |
| glycine betaine degradation III | 7 | 4 | 3 |
| felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis | 5 | 2 | 2 |
| glycine betaine degradation I | 8 | 4 | 3 |
| molybdenum cofactor biosynthesis | 3 | 3 | 1 |
| glycine degradation | 3 | 3 | 1 |
| L-methionine biosynthesis II | 6 | 5 | 2 |
| L-mimosine degradation | 8 | 4 | 2 |
| glutathione-mediated detoxification I | 8 | 3 | 2 |
| sulfide oxidation IV (mitochondria) | 5 | 2 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis II | 15 | 13 | 2 |
| purine nucleobases degradation II (anaerobic) | 24 | 17 | 3 |
| mycolate biosynthesis | 205 | 25 | 3 |
| superpathway of mycolate biosynthesis | 239 | 26 | 3 |