Experiment set13IT030 for Escherichia coli BW25113

Compare to:

LB with Nickel (II) chloride hexahydrate 1 mM

200 most important genes:

  gene name fitness t score description  
b2318 truA -4.4 -3.0 tRNA pseudouridine synthase A (NCBI) compare
b0145 dksA -3.4 -3.2 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b4242 mgtA -3.3 -9.1 magnesium transporter (NCBI) compare
b0437 clpP -2.6 -7.9 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b3644 yicC -2.4 -9.1 hypothetical protein (NCBI) compare
b3164 pnp -2.4 -3.5 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b3764 yifE -2.4 -7.1 hypothetical protein (NCBI) compare
b4702 mgtL -2.3 -2.6 regulatory leader peptide for mgtA (NCBI) compare
b0740 tolB -2.2 -5.3 translocation protein TolB precursor (NCBI) compare
b4480 hdfR -2.2 -8.0 transcriptional regulator HdfR (NCBI) compare
b3778 rep -2.1 -9.5 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b0149 mrcB -2.1 -14.2 penicillin-binding protein 1b (NCBI) compare
b0741 pal -2.1 -4.8 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b3977 tyrU -2.1 -1.5 tRNA-Tyr (NCBI) compare
b0438 clpX -2.1 -8.5 ATP-dependent protease ATP-binding subunit (NCBI) compare
b2501 ppk -2.0 -11.3 polyphosphate kinase (NCBI) compare
b1286 rnb -2.0 -3.8 exoribonuclease II (NCBI) compare
b0630 lipB -1.9 -1.8 protein of lipoate biosynthesis (VIMSS) compare
b2106 yohM -1.9 -9.5 membrane protein conferring nickel and cobalt resistance (NCBI) conserved
b2282 nuoH -1.9 -4.2 NADH dehydrogenase subunit H (NCBI) compare
b3229 sspA -1.9 -3.4 stringent starvation protein A (NCBI) compare
b2939 yqgB -1.8 -2.6 predicted protein (RefSeq) compare
b2285 nuoE -1.8 -3.2 NADH dehydrogenase subunit E (NCBI) compare
b3169 nusA -1.8 -4.5 transcription elongation factor NusA (NCBI) compare
b1105 ycfM -1.7 -5.4 predicted outer membrane lipoprotein (NCBI) compare
b0739 tolA -1.7 -3.1 cell envelope integrity inner membrane protein TolA (NCBI) compare
b1293 sapB -1.7 -1.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0949 uup -1.7 -9.8 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b3871 bipA -1.7 -11.2 GTP-binding protein (NCBI) compare
b2814 metZ -1.7 -2.6 tRNA-Met (NCBI) compare
b1865 nudB -1.7 -1.6 dATP pyrophosphohydrolase (NCBI) compare
b0738 tolR -1.6 -2.3 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b4402 yjjY -1.6 -1.6 hypothetical protein (NCBI) compare
b1235 rssB -1.6 -8.6 response regulator of RpoS (NCBI) compare
b4234 yjgA -1.6 -4.8 hypothetical protein (NCBI) compare
b4049 dusA -1.5 -8.1 tRNA-dihydrouridine synthase A (RefSeq) conserved
b0660 ybeZ -1.5 -3.8 predicted protein with nucleoside triphosphate hydrolase domain (RefSeq) compare
b0737 tolQ -1.5 -2.2 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b2288 nuoA -1.4 -3.5 NADH dehydrogenase I chain A (VIMSS) compare
b3741 gidA -1.4 -3.0 glucose-inhibited division protein A (NCBI) compare
b4171 miaA -1.4 -2.9 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b0726 sucA -1.4 -3.8 alpha-ketoglutarate decarboxylase (NCBI) compare
b3780 rhlB -1.4 -10.0 ATP-dependent RNA helicase (NCBI) compare
b2620 smpB -1.4 -1.6 SsrA-binding protein (NCBI) compare
b2528 iscA -1.3 -2.0 iron-sulfur cluster assembly protein (NCBI) compare
b3740 gidB -1.3 -8.2 glucose-inhibited division protein B (NCBI) conserved
b2938 speA -1.3 -7.6 arginine decarboxylase (NCBI) compare
b2683 ygaH -1.3 -3.5 predicted inner membrane protein (NCBI) compare
b0688 pgm -1.2 -2.9 phosphoglucomutase (NCBI) compare
b1480 sra -1.2 -2.1 30S ribosomal subunit protein S22 (NCBI) compare
b0990 cspG -1.2 -1.5 DNA-binding transcriptional regulator (NCBI) compare
b4155 yjeA -1.2 -0.8 putative lysyl-tRNA synthetase (VIMSS) compare
b0014 dnaK -1.2 -2.0 molecular chaperone DnaK (NCBI) compare
b2567 rnc -1.2 -1.3 ribonuclease III (NCBI) compare
b3290 trkA -1.2 -1.7 potassium transporter peripheral membrane component (NCBI) compare
b0439 lon -1.2 -4.3 DNA-binding ATP-dependent protease La (NCBI) compare
b1429 tehA -1.2 -1.3 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b0232 yafN -1.2 -0.7 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b3979 thrT -1.2 -0.5 tRNA-Thr (NCBI) compare
b2479 gcvR -1.2 -5.0 transcriptional regulation of gcv operon (VIMSS) compare
b0471 ybaB -1.2 -3.5 hypothetical protein (NCBI) compare
b2912 ygfA -1.2 -3.7 putative ligase (VIMSS) compare
b0564 appY -1.1 -1.4 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b4375 prfC -1.1 -3.2 peptide chain release factor 3 (NCBI) compare
b1332 ynaJ -1.1 -1.3 predicted inner membrane protein (NCBI) compare
b2576 srmB -1.1 -3.0 ATP-dependent RNA helicase (NCBI) compare
b0113 pdhR -1.1 -4.9 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b3792 wzxE -1.1 -5.7 O-antigen translocase (NCBI) compare
b3398 yrfF -1.1 -3.5 predicted inner membrane protein (NCBI) compare
b2279 nuoK -1.1 -1.5 NADH dehydrogenase subunit K (NCBI) compare
b1236 galU -1.1 -3.6 glucose-1-phosphate uridylyltransferase (NCBI) compare
b3426 glpD -1.0 -2.8 sn-glycerol-3-phosphate dehydrogenase, aerobic, FAD/NAD(P)-binding (NCBI) compare
b3613 yibP -1.0 -2.4 putative membrane protein (VIMSS) compare
b2363 yfdT -1.0 -2.1 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b3811 xerC -1.0 -1.2 site-specific tyrosine recombinase XerC (NCBI) compare
b1090 plsX -1.0 -4.4 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b2278 nuoL -1.0 -6.3 NADH dehydrogenase subunit L (NCBI) compare
b0729 sucD -1.0 -3.6 succinyl-CoA synthetase subunit alpha (NCBI) compare
b1095 fabF -1.0 -5.4 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b1842 holE -1.0 -2.3 DNA polymerase III, theta subunit (NCBI) compare
b3162 deaD -1.0 -3.7 inducible ATP-independent RNA helicase (VIMSS) compare
b2820 recB -1.0 -0.9 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b2946 yggJ -0.9 -4.7 orf, hypothetical protein (VIMSS) compare
b3107 yhaL -0.9 -1.7 orf, hypothetical protein (VIMSS) compare
b2189 proL -0.9 -1.8 tRNA-Pro (NCBI) compare
b3414 gntY -0.9 -4.5 predicted gluconate transport associated protein (NCBI) compare
b2330 yfcB -0.9 -1.0 putative adenine-specific methylase (VIMSS) compare
b0049 apaH -0.9 -2.5 diadenosinetetraphosphatase (NCBI) compare
b2937 speB -0.9 -5.0 agmatinase (NCBI) compare
b1084 rne -0.9 -3.3 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b0888 trxB -0.9 -3.1 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b3844 fre -0.9 -3.8 NAD(P)H-flavin reductase (NCBI) compare
b2504 yfgG -0.9 -0.6 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b3204 ptsN -0.9 -3.9 sugar-specific enzyme IIA component of PTS (NCBI) compare
b3706 trmE -0.9 -2.8 tRNA modification GTPase (NCBI) compare
b2168 fruK -0.9 -1.4 1-phosphofructokinase (NCBI) compare
b3962 udhA -0.9 -4.7 putative oxidoreductase (VIMSS) compare
b3649 rpoZ -0.9 -2.1 DNA-directed RNA polymerase subunit omega (NCBI) compare
b0685 ybfE -0.9 -2.4 orf, hypothetical protein (VIMSS) compare
b2020 hisD -0.9 -3.8 histidinol dehydrogenase (NCBI) compare
b1088 yceD -0.9 -3.6 hypothetical protein (NCBI) compare
b3410 yhgG -0.9 -5.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b1171 b1171 -0.8 -2.1 orf, hypothetical protein (VIMSS) compare
b2569 lepA -0.8 -3.1 GTP-binding protein LepA (NCBI) compare
b2287 nuoB -0.8 -2.8 NADH dehydrogenase subunit B (NCBI) compare
b0051 ksgA -0.8 -3.2 dimethyladenosine transferase (NCBI) compare
b3183 obgE -0.8 -1.2 GTPase involved in cell partioning and DNA repair (NCBI) compare
b3180 yhbY -0.8 -4.2 predicted RNA-binding protein (NCBI) compare
b0418 pgpA -0.8 -4.3 phosphatidylglycerophosphatase A (NCBI) compare
b0088 murD -0.8 -1.4 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b0053 surA -0.8 -3.8 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b3839 tatC -0.8 -4.4 TatABCE protein translocation system subunit (NCBI) compare
b2136 yohD -0.8 -1.8 orf, hypothetical protein (VIMSS) compare
b1676 pykF -0.8 -2.8 pyruvate kinase (NCBI) compare
b0585 fes -0.8 -4.0 enterobactin/ferric enterobactin esterase (NCBI) compare
b4482 yigE -0.8 -2.7 hypothetical protein (NCBI) compare
b4695 ykgT -0.8 -2.1 no description compare
b4593 ymgI -0.8 -0.6 hypothetical protein (RefSeq) compare
b3932 hslV -0.8 -5.2 ATP-dependent protease peptidase subunit (NCBI) compare
b1049 mdoH -0.8 -5.0 glucosyltransferase MdoH (NCBI) compare
b3042 b3042 -0.8 -3.0 orf, hypothetical protein (VIMSS) compare
b0948 ycbY -0.7 -5.0 predicted methyltransferase (NCBI) compare
b2286 nuoC -0.7 -3.1 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b2007 yeeX -0.7 -1.8 conserved protein (RefSeq) compare
b4180 rlmB -0.7 -4.0 23S rRNA (Gm2251)-methyltransferase (NCBI) compare
b1125 potB -0.7 -3.2 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b3931 hslU -0.7 -5.8 ATP-dependent protease ATP-binding subunit (NCBI) compare
b4413 sokC -0.7 -1.2 regulatory antisense RNA (NCBI) compare
b1936 intG -0.7 -0.8 predicted defective phage integrase (pseudogene) (RefSeq) compare
b0706 ybfD -0.7 -1.9 hypothetical protein (NCBI) compare
b4369 leuP -0.7 -2.3 tRNA-Leu (NCBI) compare
b2498 upp -0.7 -2.0 uracil phosphoribosyltransferase (VIMSS) compare
b1291 sapD -0.7 -1.4 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1130 phoP -0.7 -0.5 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b3742 mioC -0.7 -3.4 flavodoxin (NCBI) compare
b1860 ruvB -0.7 -0.7 Holliday junction DNA helicase B (NCBI) compare
b1864 yebC -0.7 -3.4 hypothetical protein (NCBI) compare
b2688 gshA -0.7 -4.0 glutamate--cysteine ligase (NCBI) compare
b4201 priB -0.7 -2.0 primosomal replication protein N (NCBI) compare
b3842 rfaH -0.7 -2.7 transcriptional activator RfaH (NCBI) compare
b3409 feoB -0.7 -6.2 ferrous iron transport protein B (NCBI) compare
b2110 yehC -0.7 -0.8 predicted periplasmic pilin chaperone (NCBI) compare
b3484 yhhI -0.7 -2.0 predicted transposase (NCBI) compare
b0890 ftsK -0.7 -2.2 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b1729 ydjN -0.7 -3.7 predicted transporter (NCBI) compare
b0303 ykgI -0.7 -0.4 orf, hypothetical protein (VIMSS) compare
b1040 csgD -0.7 -0.9 DNA-binding transcriptional activator in two-component regulatory system (NCBI) compare
b2023 hisH -0.7 -3.2 imidazole glycerol phosphate synthase subunit HisH (NCBI) compare
b2317 dedA -0.7 -2.8 conserved inner membrane protein (NCBI) compare
b2564 pdxJ -0.7 -2.3 pyridoxal phosphate biosynthetic protein (NCBI) compare
b1519 tam -0.7 -2.9 trans-aconitate 2-methyltransferase (NCBI) compare
b2689 yqaA -0.7 -3.2 conserved inner membrane protein (NCBI) compare
b4502 yeiW -0.7 -2.0 hypothetical protein (NCBI) compare
b3190 yrbA -0.6 -2.5 orf, hypothetical protein (VIMSS) compare
b2280 nuoJ -0.6 -2.9 NADH dehydrogenase subunit J (NCBI) compare
b3005 exbD -0.6 -2.9 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1709 btuD -0.6 -0.7 vitamin B12-transporter ATPase (NCBI) compare
b2277 nuoM -0.6 -3.1 NADH dehydrogenase subunit M (NCBI) compare
b1506 yneL -0.6 -1.2 predicted transcriptional regulator (NCBI) compare
b3860 dsbA -0.6 -4.2 periplasmic protein disulfide isomerase I (NCBI) compare
b0143 pcnB -0.6 -3.2 poly(A) polymerase I (VIMSS) compare
b2699 recA -0.6 -0.9 recombinase A (NCBI) compare
b3390 aroK -0.6 -1.6 shikimate kinase I (NCBI) compare
b0721 sdhC -0.6 -1.6 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b2291 yfbR -0.6 -2.1 hypothetical protein (NCBI) compare
b0728 sucC -0.6 -3.8 succinyl-CoA synthetase subunit beta (NCBI) compare
b3408 feoA -0.6 -4.5 ferrous iron transport protein A (NCBI) compare
b3405 ompR -0.6 -3.6 osmolarity response regulator (NCBI) compare
b4535 yniD -0.6 -0.9 predicted protein (RefSeq) compare
b3026 qseC -0.6 -2.1 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b2283 nuoG -0.6 -3.5 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b2066 udk -0.6 -3.0 uridine/cytidine kinase (VIMSS) compare
b1334 fnr -0.6 -1.7 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b4313 fimE -0.6 -4.2 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b4146 yjeK -0.6 -0.8 predicted lysine aminomutase (NCBI) compare
b0636 ybeA -0.6 -3.4 hypothetical protein (NCBI) compare
b1542 ydfI -0.6 -2.5 predicted mannonate dehydrogenase (NCBI) compare
b2276 nuoN -0.6 -2.7 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b3726 pstA -0.6 -2.6 phosphate transporter subunit (NCBI) compare
b3963 fabR -0.6 -3.9 DNA-binding transcriptional repressor (RefSeq) compare
b3348 slyX -0.6 -2.4 hypothetical protein (NCBI) compare
b4371 rsmC -0.6 -3.7 16S ribosomal RNA m2G1207 methyltransferase (NCBI) compare
b1863 ruvC -0.6 -1.0 Holliday junction resolvase (NCBI) compare
b3781 trxA -0.6 -2.3 thioredoxin 1 (VIMSS) compare
b1409 ynbB -0.6 -0.8 predicted CDP-diglyceride synthase (NCBI) compare
b1129 phoQ -0.6 -0.4 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b2804 fucU -0.6 -1.5 L-fucose mutarotase (NCBI) compare
b0542 renD -0.6 -1.1 orf, hypothetical protein (VIMSS) compare
b1001 yccE -0.6 -0.3 hypothetical protein (NCBI) compare
b0237 pepD -0.6 -3.8 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b1658 purR -0.6 -2.7 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b3166 truB -0.6 -3.6 tRNA pseudouridine synthase B (NCBI) compare
b3236 mdh -0.6 -3.5 malate dehydrogenase (NCBI) compare
b4494 arpB -0.6 -1.2 no description compare
b0191 yaeJ -0.6 -2.3 hypothetical protein (NCBI) compare
b2284 nuoF -0.5 -2.1 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b1523 yneG -0.5 -1.0 hypothetical protein (NCBI) compare
b1222 narX -0.5 -2.0 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b0908 aroA -0.5 -3.6 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b2340 sixA -0.5 -1.6 phosphohistidine phosphatase (NCBI) compare


Specific Phenotypes

For 3 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Escherichia coli BW25113

For stress Nickel (II) chloride hexahydrate across organisms