Experiment set13IT030 for Escherichia coli BW25113

Compare to:

LB with Nickel (II) chloride hexahydrate 1 mM

200 most detrimental genes:

  gene name fitness t score description  
b1493 gadB +1.5 1.4 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b3784 rfe +1.3 12.1 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b0200 gmhB +1.3 1.9 hypothetical protein (NCBI) compare
b4647 mokA +1.0 1.2 no description compare
b3863 polA +1.0 1.2 DNA polymerase I (NCBI) compare
b3816 corA +1.0 7.8 magnesium/nickel/cobalt transporter (NCBI) compare
b4461 yfjD +1.0 7.4 predicted inner membrane protein (RefSeq) conserved
b1524 yneH +0.9 1.2 predicted glutaminase (NCBI) compare
b1279 yciS +0.9 1.4 conserved inner membrane protein (NCBI) compare
b0142 folK +0.9 0.6 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b4394 yjjX +0.8 3.1 orf, hypothetical protein (VIMSS) compare
b3787 rffD +0.8 6.5 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b2830 nudH +0.8 1.3 dinucleoside polyphosphate hydrolase (NCBI) compare
b3794 rffM +0.8 5.1 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b4533 ynfO +0.8 1.9 Qin prophage; predicted protein (NCBI) compare
b4552 yrhC +0.8 0.9 no description compare
b1434 ydcN +0.8 2.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b3146 yraL +0.7 4.5 predicted methyltransferase (NCBI) conserved
b3786 rffE +0.7 6.4 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b4522 yciX_1 +0.7 2.0 hypothetical protein (NCBI) compare
b0623 cspE +0.7 0.7 cold shock protein E (NCBI) compare
b1361 ydaW +0.7 0.3 orf, hypothetical protein (VIMSS) compare
b0357 yaiN +0.7 1.8 putative alpha helix chain (VIMSS) compare
b0405 queA +0.7 4.6 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI) compare
b2990 hybG +0.7 2.0 hydrogenase 2 accessory protein (NCBI) compare
b1671 ydhX +0.7 2.1 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b1183 umuD +0.7 1.7 DNA polymerase V, subunit D (NCBI) compare
b0796 ybiH +0.7 2.4 putative transcriptional regulator (VIMSS) compare
b1823 cspC +0.7 1.6 stress protein, member of the CspA-family (NCBI) compare
b1486 ddpB +0.6 2.4 D-ala-D-ala transporter subunit (NCBI) compare
b0683 fur +0.6 0.6 ferric uptake regulator (NCBI) compare
b2071 yegJ +0.6 1.3 hypothetical protein (NCBI) compare
b2846 yqeH +0.6 0.6 orf, hypothetical protein (VIMSS) compare
b4546 ypeB +0.6 1.1 hypothetical protein (NCBI) compare
b4513 kdpF +0.6 1.6 potassium ion accessory transporter subunit (NCBI) compare
b2506 yfgI +0.6 1.6 hypothetical protein (NCBI) compare
b2258 b2258 +0.6 0.9 putative transport/receptor protein (VIMSS) compare
b4436 ryeC +0.6 1.4 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b1103 ycfF +0.6 1.3 orf, hypothetical protein (VIMSS) compare
b0249 ykfF +0.6 1.6 CP4-6 prophage; predicted protein (NCBI) compare
b1418 cybB +0.6 1.9 cytochrome b(561) (VIMSS) compare
b1953 b1953 +0.6 2.4 orf, hypothetical protein (VIMSS) compare
b1548 nohA +0.6 1.3 Qin prophage; predicted packaging protein (RefSeq) compare
b4391 yjjK +0.6 4.4 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1925 fliS +0.6 3.2 flagellar protein FliS (NCBI) compare
b1831 proQ +0.6 2.2 putative solute/DNA competence effector (NCBI) compare
b1882 cheY +0.6 0.6 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1281 pyrF +0.6 1.7 orotidine 5'-phosphate decarboxylase (NCBI) compare
b2096 gatY +0.6 2.3 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b2204 napH +0.6 2.9 quinol dehydrogenase membrane component (NCBI) compare
b1960 vsr +0.5 2.3 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b1667 ydhR +0.5 1.4 hypothetical protein (NCBI) compare
b3915 fieF +0.5 4.3 ferrous iron efflux protein F (NCBI) conserved
b2635 ypjK +0.5 2.4 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b0696 kdpC +0.5 2.2 potassium-transporting ATPase subunit C (NCBI) compare
b1677 lpp +0.5 1.4 murein lipoprotein (NCBI) compare
b1627 rsxA +0.5 1.6 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b2424 cysU +0.5 1.4 sulfate/thiosulfate transporter subunit (NCBI) compare
b4701 sokX +0.5 0.5 sok-related sRNA, function unknown (RefSeq) compare
b1837 yebW +0.5 1.3 orf, hypothetical protein (VIMSS) compare
b1164 ycgZ +0.5 1.1 hypothetical protein (NCBI) compare
b4596 yciZ +0.5 1.3 hypothetical protein (RefSeq) compare
b3034 nudF +0.5 3.2 ADP-ribose pyrophosphatase (NCBI) compare
b1150 ymfR +0.5 0.8 e14 prophage; predicted protein (NCBI) compare
b3029 ygiN +0.5 1.5 quinol monooxygenase (NCBI) compare
b3790 rffC +0.5 3.0 TDP-fucosamine acetyltransferase (RefSeq) compare
b4548 ypjJ +0.5 2.3 hypothetical protein (NCBI) compare
b4373 rimI +0.5 1.7 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b2419 yfeK +0.5 1.4 hypothetical protein (NCBI) compare
b4481 rffT +0.5 3.3 4-alpha-L-fucosyltransferase (NCBI) compare
b0641 rlpB +0.5 1.5 minor lipoprotein (NCBI) compare
b0547 ybcN +0.5 2.2 DLP12 prophage; predicted protein (NCBI) compare
b1161 ycgX +0.5 0.5 hypothetical protein (NCBI) compare
b4527 b1354 +0.5 1.7 orf, hypothetical protein (VIMSS) compare
b1901 araF +0.5 1.8 L-arabinose transporter subunit (NCBI) compare
b2422 cysA +0.5 3.5 sulfate/thiosulfate transporter subunit (NCBI) compare
b0937 ssuE +0.5 1.0 NAD(P)H-dependent FMN reductase (NCBI) compare
b1305 pspB +0.5 1.8 phage shock protein B (NCBI) compare
b1218 chaC +0.5 2.4 regulatory protein for cation transport (RefSeq) compare
b3472 b3472 +0.5 3.0 orf, hypothetical protein (VIMSS) compare
b1114 mfd +0.5 3.2 transcription-repair coupling factor (NCBI) compare
b3500 gor +0.5 0.9 glutathione reductase (NCBI) compare
b1333 uspE +0.5 1.7 stress-induced protein (NCBI) compare
b3281 aroE +0.5 4.0 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b0520 ylbF +0.5 2.5 hypothetical protein (NCBI) compare
b2111 yehD +0.5 1.3 predicted fimbrial-like adhesin protein (NCBI) compare
b2703 srlE +0.5 2.7 glucitol/sorbitol-specific enzyme IIB component of PTS (NCBI) compare
b1874 cutC +0.4 1.3 copper homeostasis protein (NCBI) compare
b1724 ydiZ +0.4 0.8 hypothetical protein (NCBI) compare
b4538 yoeF +0.4 0.8 hypothetical protein (RefSeq) compare
b1824 yobF +0.4 1.2 hypothetical protein (NCBI) compare
b3806 cyaA +0.4 2.1 adenylate cyclase (NCBI) compare
b3033 yqiB +0.4 2.3 predicted dehydrogenase (NCBI) compare
b1777 b1777 +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b2807 ygdD +0.4 2.2 conserved inner membrane protein (NCBI) compare
b1295 ymjA +0.4 1.2 hypothetical protein (NCBI) compare
b2490 b2490 +0.4 1.8 putative protein processing element (VIMSS) compare
b2459 eutT +0.4 2.7 predicted cobalamin adenosyltransferase in ethanolamine utilization (NCBI) compare
b3389 aroB +0.4 3.4 3-dehydroquinate synthase (NCBI) compare
b2711 norW +0.4 2.0 nitric oxide reductase (NCBI) compare
b3074 ygjH +0.4 2.2 hypothetical protein (NCBI) compare
b3858 yihD +0.4 1.3 hypothetical protein (NCBI) compare
b1456 rhsE +0.4 2.3 rhsE element core protein RshE (RefSeq) compare
b0953 rmf +0.4 0.9 ribosome modulation factor (NCBI) compare
b2237 inaA +0.4 1.5 hypothetical protein (NCBI) compare
b4012 yjaB +0.4 1.4 predicted acetyltransferase (NCBI) compare
b2423 cysW +0.4 1.9 sulfate/thiosulfate transporter subunit (NCBI) compare
b3504 yhiS +0.4 0.2 hypothetical protein (RefSeq) compare
b4451 ryhB +0.4 0.7 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b1511 ydeV +0.4 2.0 putative kinase (VIMSS) compare
b1610 tus +0.4 2.0 DNA replication terminus site-binding protein (NCBI) compare
b1802 yeaW +0.4 2.0 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b2928 yggC +0.4 3.0 hypothetical protein (NCBI) compare
b1313 ycjQ +0.4 2.4 predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI) compare
b1422 ydcI +0.4 2.4 putative transcriptional regulator LYSR-type (VIMSS) compare
b0387 yaiI +0.4 2.2 orf, hypothetical protein (VIMSS) compare
b2637 yfjT +0.4 1.3 CP4-57 prophage; predicted protein (NCBI) compare
b1306 pspC +0.4 1.7 DNA-binding transcriptional activator (NCBI) compare
b1180 ycgM +0.4 1.4 predicted isomerase/hydrolase (NCBI) compare
b1348 lar +0.4 0.8 Rac prophage; restriction alleviation protein (NCBI) compare
b1482 osmC +0.4 1.0 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b1961 dcm +0.4 0.9 DNA cytosine methylase (NCBI) compare
b2105 yohL +0.4 1.2 hypothetical protein (NCBI) compare
b1101 ptsG +0.4 2.8 fused glucose-specific PTS enzymes: IIB component/IIC component (NCBI) compare
b4408 csrB +0.4 1.6 CsrA-binding sRNA, antagonizing CsrA regulation (NCBI) compare
b2969 yghE +0.4 1.5 predicted secretion pathway protein, L-type protein (pseudogene) (RefSeq) compare
b1602 pntB +0.4 1.8 pyridine nucleotide transhydrogenase (NCBI) compare
b2698 recX +0.4 1.0 RecA regulator RecX (NCBI) compare
b3181 greA +0.4 2.1 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b3587 yiaW +0.4 2.2 conserved inner membrane protein (NCBI) compare
b4627 ykfL +0.4 1.4 no description compare
b1104 ycfL +0.4 0.8 hypothetical protein (NCBI) compare
b1698 ydiR +0.4 1.6 predicted electron transfer flavoprotein, FAD-binding (NCBI) compare
b4557 yidD +0.4 1.6 hypothetical protein (NCBI) compare
b1626 b1626 +0.4 2.1 orf, hypothetical protein (VIMSS) compare
b1727 yniC +0.4 1.2 predicted hydrolase (NCBI) compare
b1135 ymfC +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b0097 yacA +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b0994 torT +0.4 1.6 periplasmic sensory protein associated with the TorRS two-component regulatory system (NCBI) compare
b3868 glnG +0.4 2.0 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b2208 napF +0.4 2.0 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b1989 asnV +0.4 1.2 tRNA-Asn (NCBI) compare
b1221 narL +0.4 1.3 DNA-binding response regulator in two-component regulatory system with NarX (or NarQ) (NCBI) compare
b1553 ydfP +0.4 1.1 Qin prophage; conserved protein (NCBI) compare
b4501 torI +0.4 2.0 response regulator inhibitor for tor operon (NCBI) compare
b4519 icdC +0.4 1.4 no description compare
b1787 yeaK +0.4 2.1 hypothetical protein (NCBI) compare
b0410 yajD +0.4 2.3 hypothetical protein (NCBI) compare
b4570 lomR +0.4 0.8 no description compare
b0946 ycbW +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b3933 ftsN +0.4 2.6 essential cell division protein (NCBI) compare
b1061 dinI +0.4 1.9 DNA damage-inducible protein I (NCBI) compare
b4541 yehK +0.4 1.6 hypothetical protein (NCBI) compare
b1372 b1372 +0.4 2.2 putative membrane protein (VIMSS) compare
b4503 yafF +0.4 0.5 no description compare
b0789 ybhO +0.4 1.6 cardiolipin synthase 2 (NCBI) compare
b2301 yfcF +0.4 1.8 predicted enzyme (NCBI) compare
b0504 ybbS +0.4 1.5 DNA-binding transcriptional activator of the allD operon (NCBI) compare
b1686 ydiI +0.4 0.9 hypothetical protein (NCBI) compare
b2377 yfdY +0.3 1.1 predicted inner membrane protein (NCBI) compare
b1419 ydcA +0.3 1.5 hypothetical protein (NCBI) compare
b4609 ryfD +0.3 1.5 Novel sRNA, function unknown (RefSeq) compare
b1475 fdnH +0.3 2.0 formate dehydrogenase-N, Fe-S (beta) subunit, nitrate-inducible (NCBI) compare
b4631 ykgQ +0.3 0.7 no description compare
b4045 yjbJ +0.3 1.1 predicted stress response protein (NCBI) compare
b2741 rpoS +0.3 2.0 RNA polymerase sigma factor (NCBI) compare
b0015 dnaJ +0.3 2.6 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b1588 ynfF +0.3 1.3 oxidoreductase subunit (RefSeq) compare
b1790 yeaM +0.3 1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b2195 ccmG +0.3 1.2 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b2354 yfdK +0.3 1.7 CPS-53 (KpLE1) prophage; conserved protein (NCBI) compare
b2293 yfbT +0.3 1.2 putative phosphatase (VIMSS) compare
b3427 yzgL +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b1442 ydcU +0.3 1.8 predicted spermidine/putrescine transporter subunit (NCBI) compare
b2771 ygcS +0.3 1.8 putative transport protein (VIMSS) compare
b1635 gst +0.3 1.7 glutathionine S-transferase (NCBI) compare
b0245 ykfI +0.3 2.2 CP4-6 prophage; toxin of the YkfI-YafW toxin-antitoxin system (NCBI) compare
b1850 eda +0.3 0.9 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b1741 ydjQ +0.3 0.9 endonuclease of nucleotide excision repair (NCBI) compare
b2225 yfaP +0.3 1.5 hypothetical protein (NCBI) compare
b0959 yccR +0.3 1.8 hypothetical protein (NCBI) compare
b4190 yjfP +0.3 1.8 predicted hydrolase (NCBI) compare
b4395 ytjC +0.3 2.0 phosphoglycerate mutase (NCBI) compare
b2005 yeeV +0.3 1.5 CP4-44 prophage; toxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b1271 yciK +0.3 1.1 short chain dehydrogenase (NCBI) compare
b2977 glcG +0.3 1.4 hypothetical protein (NCBI) compare
b1464 yddE +0.3 1.6 hypothetical protein (NCBI) compare
b4630 ykgP +0.3 1.1 no description compare
b2554 yfhA +0.3 2.3 predicted DNA-binding response regulator in two-component system (NCBI) compare
b1919 yedO +0.3 1.4 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b0970 yccA +0.3 1.2 inner membrane protein (NCBI) compare
b2125 yehT +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b2769 ygcQ +0.3 1.3 putative flavoprotein (VIMSS) compare
b3966 btuB +0.3 2.8 vitamin B12/cobalamin outer membrane transporter (NCBI) compare
b3677 yidI +0.3 1.9 predicted inner membrane protein (NCBI) compare
b0426 yajQ +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b2209 eco +0.3 0.9 ecotin precursor (NCBI) compare
b1512 lsrR +0.3 1.2 lsr operon transcriptional repressor (NCBI) compare
b4184 yjfL +0.3 1.3 conserved inner membrane protein (NCBI) compare
b1728 ydjM +0.3 1.1 predicted inner membrane protein regulated by LexA (RefSeq) compare


Specific Phenotypes

For 3 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Escherichia coli BW25113

For stress Nickel (II) chloride hexahydrate across organisms