Experiment set13IT027 for Escherichia coli BW25113

Compare to:

LB with Cisplatin 0.05 mg/ml

200 most detrimental genes:

  gene name fitness t score description  
b0912 ihfB +2.2 11.4 integration host factor subunit beta (NCBI) compare
b0958 sulA +1.9 8.9 SOS cell division inhibitor (NCBI) compare
b3493 pitA +1.6 12.4 phosphate transporter, low-affinity (NCBI) compare
b1349 recT +1.6 7.7 Rac prophage; recombination and repair protein (NCBI) compare
b0059 hepA +1.5 13.2 ATP-dependent helicase HepA (NCBI) compare
b0092 ddlB +1.5 7.8 D-alanylalanine synthetase (NCBI) compare
b4538 yoeF +1.4 3.2 hypothetical protein (RefSeq) compare
b1712 ihfA +1.4 2.1 integration host factor subunit alpha (NCBI) compare
b3229 sspA +1.3 5.0 stringent starvation protein A (NCBI) compare
b1348 lar +1.3 2.8 Rac prophage; restriction alleviation protein (NCBI) compare
b1231 tyrT +1.3 1.6 tRNA-Tyr (NCBI) compare
b2906 visC +1.2 7.0 hypothetical protein (NCBI) compare
b1984 asnW +1.2 2.0 tRNA-Asn (NCBI) compare
b4163 glyV +1.2 1.8 tRNA-Gly (NCBI) compare
b2518 ndk +1.2 4.8 nucleoside diphosphate kinase (NCBI) compare
b3806 cyaA +1.1 6.2 adenylate cyclase (NCBI) compare
b4701 sokX +1.1 1.3 sok-related sRNA, function unknown (RefSeq) compare
b2285 nuoE +1.1 3.3 NADH dehydrogenase subunit E (NCBI) compare
b0428 cyoE +1.1 6.7 protoheme IX farnesyltransferase (NCBI) compare
b0088 murD +1.0 1.8 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b2479 gcvR +1.0 4.8 transcriptional regulation of gcv operon (VIMSS) compare
b3844 fre +1.0 5.5 NAD(P)H-flavin reductase (NCBI) compare
b0632 dacA +1.0 7.2 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b1493 gadB +1.0 0.8 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b3979 thrT +1.0 0.6 tRNA-Thr (NCBI) compare
b1804 rnd +1.0 4.0 ribonuclease D (NCBI) compare
b2279 nuoK +0.9 2.3 NADH dehydrogenase subunit K (NCBI) compare
b0437 clpP +0.9 5.4 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b2528 iscA +0.9 2.2 iron-sulfur cluster assembly protein (NCBI) compare
b4596 yciZ +0.9 2.1 hypothetical protein (RefSeq) compare
b0438 clpX +0.9 5.9 ATP-dependent protease ATP-binding subunit (NCBI) compare
b1765 ydjA +0.9 1.2 predicted oxidoreductase (NCBI) compare
b0357 yaiN +0.9 2.3 putative alpha helix chain (VIMSS) compare
b2284 nuoF +0.9 4.2 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b2280 nuoJ +0.9 5.1 NADH dehydrogenase subunit J (NCBI) compare
b1361 ydaW +0.8 0.3 orf, hypothetical protein (VIMSS) compare
b0432 cyoA +0.8 6.1 cytochrome o ubiquinol oxidase subunit II (NCBI) compare
b2299 yfcD +0.8 3.7 predicted NUDIX hydrolase (NCBI) compare
b1235 rssB +0.8 4.7 response regulator of RpoS (NCBI) compare
b4704 arrS +0.8 0.4 Antisense sRNA ArrS, function unknown (NCBI) compare
b1874 cutC +0.8 2.4 copper homeostasis protein (NCBI) compare
b2909 ygfB +0.8 5.0 orf, hypothetical protein (VIMSS) compare
b1104 ycfL +0.8 1.7 hypothetical protein (NCBI) compare
b0720 gltA +0.8 4.3 citrate synthase (NCBI) compare
b3398 yrfF +0.8 2.8 predicted inner membrane protein (NCBI) compare
b4522 yciX_1 +0.8 2.1 hypothetical protein (NCBI) compare
b2911 ssrS +0.8 1.5 6S regulatory RNA (NCBI) compare
b0429 cyoD +0.7 3.6 cytochrome o ubiquinol oxidase subunit IV (NCBI) compare
b1850 eda +0.7 1.5 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b1160 elbA +0.7 0.5 hypothetical protein (NCBI) compare
b2237 inaA +0.7 2.6 hypothetical protein (NCBI) compare
b0081 mraZ +0.7 3.8 hypothetical protein (NCBI) compare
b3414 gntY +0.7 3.4 predicted gluconate transport associated protein (NCBI) compare
b1109 ndh +0.7 3.0 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b4012 yjaB +0.7 1.5 predicted acetyltransferase (NCBI) compare
b0989 cspH +0.7 1.7 stress protein, member of the CspA-family (NCBI) compare
b2216 rcsD +0.7 5.6 phosphotransfer intermediate protein in two-component regulatory system with RcsBC (NCBI) compare
b2502 ppx +0.7 5.0 exopolyphosphatase (NCBI) compare
b3871 bipA +0.7 5.8 GTP-binding protein (NCBI) compare
b4519 icdC +0.7 2.7 no description compare
b1777 b1777 +0.7 2.4 orf, hypothetical protein (VIMSS) compare
b3129 sohA +0.6 2.1 predicted regulator (NCBI) compare
b4394 yjjX +0.6 1.7 orf, hypothetical protein (VIMSS) compare
b2283 nuoG +0.6 3.8 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b2258 b2258 +0.6 1.0 putative transport/receptor protein (VIMSS) compare
b2111 yehD +0.6 1.9 predicted fimbrial-like adhesin protein (NCBI) compare
b0853 ybjN +0.6 3.4 predicted oxidoreductase (NCBI) compare
b2278 nuoL +0.6 4.4 NADH dehydrogenase subunit L (NCBI) compare
b1379 hslJ +0.6 2.3 heat-inducible protein (NCBI) compare
b4598 yncL +0.6 1.9 hypothetical protein (RefSeq) compare
b1461 ydcE +0.6 2.0 4-oxalocrotonate tautomerase (NCBI) compare
b2281 nuoI +0.6 1.9 NADH dehydrogenase subunit I (NCBI) compare
b2293 yfbT +0.6 1.9 putative phosphatase (VIMSS) compare
b1418 cybB +0.6 2.2 cytochrome b(561) (VIMSS) compare
b2377 yfdY +0.6 2.1 predicted inner membrane protein (NCBI) compare
b1671 ydhX +0.6 1.1 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b2277 nuoM +0.6 3.5 NADH dehydrogenase subunit M (NCBI) compare
b1840 yebZ +0.6 2.7 predicted inner membrane protein (NCBI) compare
b2144 sanA +0.6 4.7 hypothetical protein (NCBI) compare
b2814 metZ +0.6 1.5 tRNA-Met (NCBI) compare
b1573 ydfC +0.6 1.1 Qin prophage; predicted protein (RefSeq) compare
b4418 sraB +0.6 1.4 unknown RNA (NCBI) compare
b3766 ilvL +0.6 2.7 ilvG operon leader peptide (NCBI) compare
b2711 norW +0.6 3.5 nitric oxide reductase (NCBI) compare
b4686 yshB +0.6 2.3 expressed protein (RefSeq) compare
b1739 osmE +0.6 1.3 DNA-binding transcriptional activator (NCBI) compare
b1919 yedO +0.6 2.8 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b0212 gloB +0.6 2.2 predicted hydroxyacylglutathione hydrolase (NCBI) compare
b1307 pspD +0.6 1.4 peripheral inner membrane phage-shock protein (NCBI) compare
b2948 yqgE +0.6 2.5 orf, hypothetical protein (VIMSS) compare
b4042 dgkA +0.6 1.6 diacylglycerol kinase (NCBI) compare
b1433 b1433 +0.6 2.3 putative membrane transport protein (VIMSS) compare
b1696 ydiP +0.6 1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b1217 chaB +0.6 1.2 cation transport regulator (NCBI) compare
b1281 pyrF +0.6 2.1 orotidine 5'-phosphate decarboxylase (NCBI) compare
b2635 ypjK +0.6 2.3 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b0431 cyoB +0.6 4.3 cytochrome o ubiquinol oxidase subunit I (NCBI) compare
b1476 fdnI +0.5 0.7 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b1247 oppF +0.5 2.3 oligopeptide transporter subunit (NCBI) compare
b2586 yfiM +0.5 1.5 predicted protein (RefSeq) compare
b2286 nuoC +0.5 2.7 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b2218 rcsC +0.5 4.3 hybrid sensory kinase in two-component regulatory system with RcsB and YojN (RefSeq) compare
b3920 yiiQ +0.5 3.4 hypothetical protein (NCBI) compare
b2576 srmB +0.5 1.9 ATP-dependent RNA helicase (NCBI) compare
b4581 ybeM +0.5 2.5 no description compare
b0167 glnD +0.5 2.4 PII uridylyl-transferase (NCBI) compare
b3834 yigP +0.5 3.3 hypothetical protein (NCBI) compare
b4676 yoaK +0.5 0.4 expressed protein (RefSeq) compare
b3761 trpT +0.5 1.3 tRNA-Trp (NCBI) compare
b4532 yncN +0.5 1.4 orf, hypothetical protein (VIMSS) compare
b1083 flgL +0.5 2.3 flagellar hook-associated protein L (NCBI) compare
b2071 yegJ +0.5 1.0 hypothetical protein (NCBI) compare
b3858 yihD +0.5 1.9 hypothetical protein (NCBI) compare
b2204 napH +0.5 2.1 quinol dehydrogenase membrane component (NCBI) compare
b4570 lomR +0.5 0.9 no description compare
b3742 mioC +0.5 2.9 flavodoxin (NCBI) compare
b2674 nrdI +0.5 1.7 hypothetical protein (NCBI) compare
b4585 sroB +0.5 0.9 Novel sRNA, function unknown (RefSeq) compare
b2445 yffN +0.5 1.7 CPZ-55 prophage; predicted protein (RefSeq) compare
b1726 yniB +0.5 1.2 predicted inner membrane protein (NCBI) compare
b1741 ydjQ +0.5 2.1 endonuclease of nucleotide excision repair (NCBI) compare
b2480 bcp +0.5 2.4 thioredoxin-dependent thiol peroxidase (NCBI) compare
b4502 yeiW +0.5 2.2 hypothetical protein (NCBI) compare
b2703 srlE +0.5 2.8 glucitol/sorbitol-specific enzyme IIB component of PTS (NCBI) compare
b1012 b1012 +0.5 1.6 orf, hypothetical protein (VIMSS) compare
b3134 agaW +0.5 1.9 N-acetylgalactosamine-specific enzyme IIC component of PTS, fragment (pseudogene) (RefSeq) compare
b2007 yeeX +0.5 0.9 conserved protein (RefSeq) compare
b4345 mcrC +0.5 0.6 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b1242 ychE +0.5 2.3 predicted inner membrane protein (NCBI) compare
b1313 ycjQ +0.5 2.3 predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI) compare
b1013 ycdC +0.5 1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b3271 yhdZ +0.5 2.6 predicted amino-acid transporter subunit (NCBI) compare
b2700 ygaD +0.5 2.5 competence damage-inducible protein A (NCBI) compare
b3221 yhcH +0.5 2.6 hypothetical protein (NCBI) compare
b0146 sfsA +0.5 2.7 sugar fermentation stimulation protein A (NCBI) compare
b0226 dinJ +0.4 2.4 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b1925 fliS +0.4 1.7 flagellar protein FliS (NCBI) compare
b4436 ryeC +0.4 0.6 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b2276 nuoN +0.4 2.4 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b0746 valZ +0.4 1.6 tRNA-Val (NCBI) compare
b1486 ddpB +0.4 1.1 D-ala-D-ala transporter subunit (NCBI) compare
b1357 ydaS +0.4 1.1 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1138 ymfE +0.4 0.8 e14 prophage; predicted inner membrane protein (NCBI) compare
b2469 narQ +0.4 3.1 sensory histidine kinase in two-component regulatory system with NarP (NarL) (NCBI) compare
b1908 yecA +0.4 1.9 conserved metal-binding protein (NCBI) compare
b1597 asr +0.4 1.9 acid shock protein (VIMSS) compare
b1286 rnb +0.4 1.2 exoribonuclease II (NCBI) compare
b1810 yoaC +0.4 2.6 predicted protein (RefSeq) compare
b2399 yfeD +0.4 2.3 predicted DNA-binding transcriptional regulator (RefSeq) compare
b4444 rygA +0.4 1.8 unknown RNA (NCBI) compare
b3537 yhjT +0.4 2.3 orf, hypothetical protein (VIMSS) compare
b3427 yzgL +0.4 2.2 orf, hypothetical protein (VIMSS) compare
b2287 nuoB +0.4 1.7 NADH dehydrogenase subunit B (NCBI) compare
b1512 lsrR +0.4 1.9 lsr operon transcriptional repressor (NCBI) compare
b3210 arcB +0.4 2.7 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b1569 dicC +0.4 1.0 Qin prophage; DNA-binding transcriptional regulator for DicB (NCBI) compare
b0846 ybjK +0.4 2.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b1837 yebW +0.4 0.8 orf, hypothetical protein (VIMSS) compare
b3624 rfaZ +0.4 1.1 lipopolysaccharide core biosynthesis protein (NCBI) compare
b2867 xdhB +0.4 2.3 xanthine dehydrogenase, FAD-binding subunit (NCBI) compare
b2300 yfcE +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b0806 ybiM +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b4207 fklB +0.4 2.4 FKBP-type 22KD peptidyl-prolyl cis-trans isomerase (rotamase) (VIMSS) compare
b0102 yacF +0.4 2.2 hypothetical protein (NCBI) compare
b2040 rfbD +0.4 3.2 dTDP-4-dehydrorhamnose reductase subunit, NAD(P)-binding, of dTDP-L-rhamnose synthase (NCBI) compare
b4227 ytfQ +0.4 2.3 predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b1489 b1489 +0.4 2.2 putative enzyme (VIMSS) compare
b1308 pspE +0.4 1.7 thiosulfate:cyanide sulfurtransferase (rhodanese) (NCBI) compare
b1917 yecC +0.4 1.1 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b1475 fdnH +0.4 1.6 formate dehydrogenase-N, Fe-S (beta) subunit, nitrate-inducible (NCBI) compare
b1456 rhsE +0.4 2.0 rhsE element core protein RshE (RefSeq) compare
b4234 yjgA +0.4 1.7 hypothetical protein (NCBI) compare
b2561 yfhH +0.4 2.2 orf, hypothetical protein (VIMSS) compare
b1171 b1171 +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b4180 rlmB +0.4 2.5 23S rRNA (Gm2251)-methyltransferase (NCBI) compare
b3071 yqjI +0.4 1.8 predicted transcriptional regulator (NCBI) compare
b4613 dinQ +0.4 1.0 Damage inducible, function unknown (RefSeq) compare
b1450 b1450 +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b1246 oppD +0.4 2.2 oligopeptide transporter ATP-binding component (NCBI) compare
b2506 yfgI +0.4 1.1 hypothetical protein (NCBI) compare
b1165 ymgA +0.4 0.2 hypothetical protein (NCBI) compare
b1839 yebY +0.4 1.2 hypothetical protein (NCBI) compare
b1377 ompN +0.4 1.8 outer membrane pore protein N, non-specific (NCBI) compare
b2378 ddg +0.4 1.8 putative heat shock protein (VIMSS) compare
b0430 cyoC +0.4 1.8 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b3425 glpE +0.4 2.1 thiosulfate sulfurtransferase (NCBI) compare
b4381 deoC +0.4 1.7 deoxyribose-phosphate aldolase (NCBI) compare
b1244 oppB +0.4 2.6 oligopeptide permease ABC transporter membrane protein (NCBI) compare
b0934 ycbM +0.4 1.2 putative transport system permease protein (VIMSS) compare
b1948 fliP +0.4 1.6 flagellar biosynthesis protein P (NCBI) compare
b0225 yafQ +0.4 1.5 predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI) compare
b3810 yigA +0.4 2.7 hypothetical protein (NCBI) compare
b2969 yghE +0.4 1.6 predicted secretion pathway protein, L-type protein (pseudogene) (RefSeq) compare
b4561 insM +0.4 1.9 transposase (RefSeq) compare
b0752 zitB +0.4 2.3 zinc transporter ZitB (NCBI) compare
b2443 yffL +0.4 1.7 CPZ-55 prophage; predicted protein (NCBI) compare
b2481 hyfA +0.4 1.7 hydrogenase 4 Fe-S subunit (VIMSS) compare
b1161 ycgX +0.4 0.3 hypothetical protein (NCBI) compare
b2743 pcm +0.4 1.8 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b0964 yccT +0.4 1.6 hypothetical protein (NCBI) compare


Specific Phenotypes

For 11 genes in this experiment

For stress Cisplatin in Escherichia coli BW25113

For stress Cisplatin across organisms