Experiment set13IT016 for Escherichia coli BW25113

LB with Nalidixic acid sodium salt 0.06 mM

Group: stress
Media: LB + Nalidixic acid sodium salt (0.06 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Keio_ML9a, 24-well transparent microplate; Multitron, Aerobic, at 37 (C), shaken=700 rpm
Growth: about 5.6 generations
By: Mark on 26-Aug-16
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 12 genes in this experiment

For stress Nalidixic acid sodium salt in Escherichia coli BW25113

For stress Nalidixic acid sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, bacterial	2
Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes	2
DNA-replication	1
DNA repair, bacterial DinG and relatives	1
Selenocysteine metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Selenoamino acid metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
L-selenocysteine biosynthesis I (bacteria)	3	3	1
L-selenocysteine biosynthesis II (archaea and eukaryotes)	4	2	1
polyisoprenoid biosynthesis (E. coli)	5	5	1