Experiment set13IT016 for Escherichia coli BW25113

LB with Nalidixic acid sodium salt 0.06 mM

Group: stress
Media: LB + Nalidixic acid sodium salt (0.06 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Keio_ML9a, 24-well transparent microplate; Multitron, Aerobic, at 37 (C), shaken=700 rpm
Growth: about 5.6 generations
By: Mark on 26-Aug-16
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 12 genes in this experiment

For stress Nalidixic acid sodium salt in Escherichia coli BW25113

For stress Nalidixic acid sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, bacterial	2
Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes	2
DNA-replication	1
DNA repair, bacterial DinG and relatives	1
Heat shock dnaK gene cluster extended	1
Selenocysteine metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Selenoamino acid metabolism
• Lipopolysaccharide biosynthesis
• Thiamine metabolism
• Folate biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
L-selenocysteine biosynthesis I (bacteria)	3	3	1
UTP and CTP dephosphorylation I	7	5	2
L-selenocysteine biosynthesis II (archaea and eukaryotes)	4	2	1
polyisoprenoid biosynthesis (E. coli)	5	5	1
pyrimidine deoxyribonucleotides de novo biosynthesis IV	7	6	1
pyrimidine deoxyribonucleotides biosynthesis from CTP	8	6	1
pyrimidine deoxyribonucleotides de novo biosynthesis I	9	9	1
pyrimidine deoxyribonucleotides de novo biosynthesis III	9	8	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli)	14	14	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis	18	18	1