Experiment set12IT043 for Pseudomonas syringae pv. syringae B728a ΔmexB

KB with Harmine 10 mM

Group: stress
Media: KB + Harmine (10 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: SyringaeB728a_mexBdelta_ML3, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
By: Tyler Helmann on 9/12/19
Media components: 10 g/L Bacto Peptone, 1.5 g/L Potassium phosphate dibasic, 15 g/L Glycerol, 0.6 g/L Magnesium sulfate

Specific Phenotypes

For 4 genes in this experiment

For stress Harmine in Pseudomonas syringae pv. syringae B728a ΔmexB

For stress Harmine across organisms

SEED Subsystems

Subsystem	#Specific
Choline and Betaine Uptake and Betaine Biosynthesis	1
Peptidoglycan Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Glycine, serine and threonine metabolism
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Lipopolysaccharide biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glycine betaine biosynthesis I (Gram-negative bacteria)	2	2	1
choline degradation I	2	2	1
glycine betaine biosynthesis II (Gram-positive bacteria)	2	2	1
choline-O-sulfate degradation	3	3	1