Experiment set12IT043 for Escherichia coli BW25113

Compare to:

LB with Trimethoprim 0.0025 mM

200 most detrimental genes:

  gene name fitness t score description  
b0685 ybfE +8.1 25.0 orf, hypothetical protein (VIMSS) compare
b3390 aroK +6.3 26.4 shikimate kinase I (NCBI) compare
b1530 marR +6.2 7.1 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b3860 dsbA +5.9 46.3 periplasmic protein disulfide isomerase I (NCBI) compare
b0464 acrR +5.8 40.1 DNA-binding transcriptional repressor (NCBI) compare
b3162 deaD +5.6 22.2 inducible ATP-independent RNA helicase (VIMSS) compare
b4685 yrbN +5.0 10.2 expressed protein (RefSeq) compare
b1185 dsbB +4.7 21.8 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b3493 pitA +4.6 32.5 phosphate transporter, low-affinity (NCBI) compare
b2814 metZ +4.6 15.9 tRNA-Met (NCBI) compare
b4414 tff +4.5 18.7 unknown RNA (NCBI) compare
b0439 lon +4.2 22.5 DNA-binding ATP-dependent protease La (NCBI) compare
b0051 ksgA +4.0 16.1 dimethyladenosine transferase (NCBI) compare
b2948 yqgE +3.4 3.2 orf, hypothetical protein (VIMSS) compare
b4042 dgkA +3.3 9.5 diacylglycerol kinase (NCBI) compare
b2688 gshA +3.3 22.2 glutamate--cysteine ligase (NCBI) compare
b2684 mprA +3.3 13.3 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b3247 cafA +3.2 27.6 bundles of cytoplasmic filaments (VIMSS) compare
b2330 yfcB +3.2 7.5 putative adenine-specific methylase (VIMSS) compare
b1804 rnd +3.1 13.0 ribonuclease D (NCBI) compare
b2620 smpB +3.1 6.8 SsrA-binding protein (NCBI) compare
b2531 iscR +3.0 5.1 DNA-binding transcriptional repressor (NCBI) compare
b4146 yjeK +2.9 12.0 predicted lysine aminomutase (NCBI) compare
b3934 cytR +2.9 25.3 DNA-binding transcriptional dual regulator (NCBI) compare
b3398 yrfF +2.8 6.3 predicted inner membrane protein (NCBI) compare
b4005 purD +2.6 21.1 phosphoribosylamine--glycine ligase (NCBI) compare
b2312 purF +2.5 16.1 amidophosphoribosyltransferase (NCBI) compare
b3673 emrD +2.5 20.7 2-module integral membrane pump; multidrug resistance (VIMSS) compare
b4203 rplI +2.5 15.7 50S ribosomal protein L9 (NCBI) compare
b0564 appY +2.4 2.3 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b3261 fis +2.3 6.6 DNA-binding protein Fis (NCBI) compare
b3761 trpT +2.3 7.5 tRNA-Trp (NCBI) compare
b0746 valZ +2.3 9.0 tRNA-Val (NCBI) compare
b2947 gshB +2.3 14.5 glutathione synthetase (NCBI) compare
b4375 prfC +2.2 11.6 peptide chain release factor 3 (NCBI) compare
b3781 trxA +2.2 8.2 thioredoxin 1 (VIMSS) compare
b2040 rfbD +2.1 17.5 dTDP-4-dehydrorhamnose reductase subunit, NAD(P)-binding, of dTDP-L-rhamnose synthase (NCBI) compare
b2557 purL +2.1 17.0 phosphoribosylformylglycinamidine synthase (NCBI) compare
b2216 rcsD +2.1 10.0 phosphotransfer intermediate protein in two-component regulatory system with RcsBC (NCBI) compare
b2618 b2618 +2.1 8.3 orf, hypothetical protein (VIMSS) compare
b1292 sapC +2.1 4.5 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3764 yifE +2.1 6.0 hypothetical protein (NCBI) compare
b4480 hdfR +2.0 9.2 transcriptional regulator HdfR (NCBI) compare
b2570 rseC +2.0 13.5 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b1290 sapF +2.0 4.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1632 rsxE +2.0 11.0 NADH-ubiquinone oxidoreductase (NCBI) compare
b3190 yrbA +2.0 3.7 orf, hypothetical protein (VIMSS) compare
b2765 ygcM +2.0 7.7 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) (NCBI) compare
b4000 hupA +2.0 12.8 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b3204 ptsN +1.9 7.9 sugar-specific enzyme IIA component of PTS (NCBI) compare
b3129 sohA +1.9 4.7 predicted regulator (NCBI) compare
b1630 rsxD +1.9 9.4 electron transport complex protein RnfD (NCBI) compare
b2911 ssrS +1.9 2.3 6S regulatory RNA (NCBI) compare
b1291 sapD +1.9 3.5 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2499 purM +1.9 9.3 phosphoribosylaminoimidazole synthetase (NCBI) compare
b2041 rfbB +1.9 15.3 dTDP-glucose 4,6 dehydratase, NAD(P)-binding (NCBI) compare
b2218 rcsC +1.9 13.8 hybrid sensory kinase in two-component regulatory system with RcsB and YojN (RefSeq) compare
b2214 yojL +1.8 12.6 predicted thiamine biosynthesis lipoprotein (NCBI) compare
b0955 ycbZ +1.8 11.1 predicted peptidase (NCBI) compare
b1629 rsxC +1.8 12.1 electron transport complex protein RnfC (NCBI) compare
b2066 udk +1.8 9.3 uridine/cytidine kinase (VIMSS) compare
b4391 yjjK +1.7 12.6 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b3785 wzzE +1.7 12.4 putative transport protein (VIMSS) compare
b4155 yjeA +1.7 2.6 putative lysyl-tRNA synthetase (VIMSS) compare
b2476 purC +1.7 5.5 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b1628 rsxB +1.7 4.5 electron transport complex protein RnfB (NCBI) compare
b0471 ybaB +1.7 7.9 hypothetical protein (NCBI) compare
b3977 tyrU +1.7 2.8 tRNA-Tyr (NCBI) compare
b3228 sspB +1.7 4.9 ClpXP protease specificity-enhancing factor (NCBI) compare
b1831 proQ +1.6 6.6 putative solute/DNA competence effector (NCBI) compare
b1766 sppA +1.6 10.7 protease IV (signal peptide peptidase) (NCBI) conserved
b3871 bipA +1.6 14.0 GTP-binding protein (NCBI) compare
b3706 trmE +1.6 7.9 tRNA modification GTPase (NCBI) compare
b0523 purE +1.6 4.5 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b0449 mdlB +1.6 11.9 fused predicted multidrug transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b3741 gidA +1.6 9.3 glucose-inhibited division protein A (NCBI) compare
b1109 ndh +1.5 7.7 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b1758 b1758 +1.5 2.3 putative cytochrome oxidase (VIMSS) compare
b0014 dnaK +1.5 3.3 molecular chaperone DnaK (NCBI) compare
b0145 dksA +1.5 4.1 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b0122 yacC +1.5 6.3 orf, hypothetical protein (VIMSS) compare
b1048 mdoG +1.5 8.5 glucan biosynthesis protein, periplasmic (NCBI) compare
b2938 speA +1.5 11.4 arginine decarboxylase (NCBI) compare
b1606 folM +1.4 9.7 short chain dehydrogenase (NCBI) compare
b2038 rfbC +1.4 8.9 dTDP-4-deoxyrhamnose-3,5-epimerase (NCBI) compare
b3166 truB +1.4 8.7 tRNA pseudouridine synthase B (NCBI) compare
b3636 rpmG +1.4 4.8 50S ribosomal protein L33 (NCBI) compare
b2564 pdxJ +1.4 4.1 pyridoxal phosphate biosynthetic protein (NCBI) compare
b1627 rsxA +1.4 6.1 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b2749 ygbE +1.4 8.9 conserved inner membrane protein (NCBI) compare
b1709 btuD +1.4 1.3 vitamin B12-transporter ATPase (NCBI) compare
b3389 aroB +1.4 5.2 3-dehydroquinate synthase (NCBI) compare
b2796 sdaC +1.4 11.8 predicted serine transporter (NCBI) compare
b2340 sixA +1.4 6.7 phosphohistidine phosphatase (NCBI) compare
b1977 asnT +1.4 4.9 tRNA-Asn (NCBI) compare
b0448 mdlA +1.3 10.7 fused predicted multidrug transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1049 mdoH +1.3 10.1 glucosyltransferase MdoH (NCBI) compare
b0200 gmhB +1.3 2.8 hypothetical protein (NCBI) compare
b1001 yccE +1.3 1.1 hypothetical protein (NCBI) compare
b2318 truA +1.3 4.2 tRNA pseudouridine synthase A (NCBI) compare
b2937 speB +1.2 7.9 agmatinase (NCBI) compare
b2946 yggJ +1.2 6.2 orf, hypothetical protein (VIMSS) compare
b2502 ppx +1.2 7.8 exopolyphosphatase (NCBI) compare
b0009 mog +1.2 7.3 molybdenum cofactor biosynthesis protein (NCBI) compare
b0114 aceE +1.2 1.4 pyruvate dehydrogenase subunit E1 (NCBI) compare
b1961 dcm +1.2 2.4 DNA cytosine methylase (NCBI) compare
b1551 b1551 +1.2 0.6 orf, hypothetical protein (VIMSS) compare
b1222 narX +1.2 3.8 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b1576 ydfD +1.2 0.6 Qin prophage; predicted protein (NCBI) compare
b0357 yaiN +1.2 1.8 putative alpha helix chain (VIMSS) compare
b1499 ydeO +1.2 0.6 predicted DNA-binding transcriptional acfivator (NCBI) compare
b1524 yneH +1.2 1.2 predicted glutaminase (NCBI) compare
b1334 fnr +1.1 2.7 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b1691 ydiN +1.1 4.0 predicted transporter (RefSeq) compare
b4699 fnrS +1.1 1.0 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1347 ydaC +1.1 0.5 Rac prophage; predicted protein (NCBI) compare
b1293 sapB +1.1 1.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3466 yhhL +1.1 6.1 conserved inner membrane protein (NCBI) compare
b2042 galF +1.1 6.5 predicted subunit with GalU (NCBI) compare
b0888 trxB +1.1 4.1 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b0118 acnB +1.1 1.4 aconitate hydratase (NCBI) compare
b3465 yhhF +1.1 6.4 predicted methyltransferase (NCBI) compare
b1989 asnV +1.1 3.2 tRNA-Asn (NCBI) compare
b0082 mraW +1.1 4.8 S-adenosyl-methyltransferase (NCBI) compare
b3500 gor +1.1 2.8 glutathione reductase (NCBI) compare
b3229 sspA +1.1 3.7 stringent starvation protein A (NCBI) compare
b3290 trkA +1.1 3.3 potassium transporter peripheral membrane component (NCBI) compare
b0440 hupB +1.1 5.8 HU, DNA-binding transcriptional regulator, beta subunit (NCBI) compare
b2319 usg +1.1 2.5 hypothetical protein (NCBI) compare
b3649 rpoZ +1.0 1.7 DNA-directed RNA polymerase subunit omega (NCBI) compare
b2313 cvpA +1.0 3.4 membrane protein required for colicin V production (NCBI) compare
b1363 trkG +1.0 0.6 Rac prophage; potassium transporter subunit (NCBI) compare
b0059 hepA +1.0 9.0 ATP-dependent helicase HepA (NCBI) compare
b3181 greA +1.0 4.0 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b3258 panF +1.0 7.6 sodium/pantothenate symporter (VIMSS) compare
b1409 ynbB +1.0 0.6 predicted CDP-diglyceride synthase (NCBI) compare
b2682 ygaZ +1.0 2.6 predicted transporter (NCBI) compare
b3778 rep +1.0 7.1 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b1351 racC +1.0 0.6 Rac prophage; predicted protein (NCBI) compare
b1540 ydfH +1.0 0.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b3071 yqjI +1.0 3.0 predicted transcriptional regulator (NCBI) compare
b3244 tldD +1.0 7.6 predicted peptidase (NCBI) compare
b1558 cspF +1.0 1.8 Qin prophage; cold shock protein (NCBI) compare
b1259 yciG +1.0 1.9 orf, hypothetical protein (VIMSS) compare
b1497 b1497 +0.9 0.6 putative enzyme (VIMSS) compare
b4235 pmbA +0.9 7.2 predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 (NCBI) compare
b4392 slt +0.9 7.8 soluble lytic murein transglycosylase (VIMSS) compare
b2114 metG +0.9 2.0 methionyl-tRNA synthetase (NCBI) compare
b2017 yefM +0.9 2.5 orf, hypothetical protein (VIMSS) compare
b1572 ydfB +0.9 1.5 orf, hypothetical protein (VIMSS) compare
b1550 b1550 +0.9 1.1 orf, hypothetical protein (VIMSS) compare
b0401 brnQ +0.9 6.2 predicted branched chain amino acid transporter (LIV-II) (NCBI) compare
b0851 nfsA +0.9 3.6 nitroreductase A, NADPH-dependent, FMN-dependent (NCBI) compare
b0426 yajQ +0.9 6.0 orf, hypothetical protein (VIMSS) compare
b1536 ydeI +0.9 0.6 hypothetical protein (NCBI) compare
b1690 ydiM +0.9 1.5 predicted transporter (NCBI) compare
b1167 ymgC +0.9 0.4 hypothetical protein (NCBI) compare
b1589 ynfG +0.9 1.7 oxidoreductase, Fe-S subunit (NCBI) compare
b3789 rffH +0.9 3.2 glucose-1-phosphate thymidylyltransferase (NCBI) compare
b2652 ileY +0.9 1.0 tRNA-Ile (NCBI) compare
b1332 ynaJ +0.9 0.8 predicted inner membrane protein (NCBI) compare
b2143 cdd +0.9 4.0 cytidine deaminase (NCBI) compare
b3149 yraO +0.9 5.3 DnaA initiator-associating factor for replication initiation (NCBI) compare
b1772 b1772 +0.9 3.2 putative kinase (VIMSS) compare
b0782 moaB +0.9 1.7 molybdopterin biosynthesis protein B (NCBI) compare
b2812 ygdL +0.9 5.4 hypothetical protein (NCBI) compare
b4494 arpB +0.8 1.4 no description compare
b0310 ykgH +0.8 1.4 predicted inner membrane protein (NCBI) compare
b0707 ybgA +0.8 2.9 hypothetical protein (NCBI) compare
b1159 mcrA +0.8 1.0 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b4594 ymgJ +0.8 2.0 hypothetical protein (RefSeq) compare
b4139 aspA +0.8 6.3 aspartate ammonia-lyase (aspartase) (VIMSS) compare
b1545 pinQ +0.8 0.3 Qin prophage; predicted site-specific recombinase (NCBI) compare
b1007 b1007 +0.8 1.8 orf, hypothetical protein (VIMSS) compare
b3774 ilvC +0.8 6.8 ketol-acid reductoisomerase (NCBI) compare
b4381 deoC +0.8 3.5 deoxyribose-phosphate aldolase (NCBI) compare
b1121 ycfZ +0.8 1.4 predicted inner membrane protein (NCBI) compare
b1257 yciE +0.8 0.5 hypothetical protein (NCBI) compare
b1871 yecP +0.8 3.1 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b3672 ivbL +0.8 3.1 ilvB operon leader peptide (NCBI) compare
b2707 srlR +0.8 2.7 DNA-bindng transcriptional repressor (NCBI) compare
b3009 yghB +0.8 5.7 conserved inner membrane protein (NCBI) compare
b1298 ycjL +0.8 1.6 probable amidotransferase subunit (VIMSS) compare
b1648 b1648 +0.8 1.2 orf, hypothetical protein (VIMSS) compare
b0797 rhlE +0.8 4.3 RNA helicase (NCBI) compare
b0418 pgpA +0.8 4.0 phosphatidylglycerophosphatase A (NCBI) compare
b0705 ybfL +0.8 0.7 putative receptor protein (VIMSS) compare
b0931 pncB +0.8 4.2 nicotinate phosphoribosyltransferase (NCBI) compare
b1767 ansA +0.8 1.9 cytoplasmic asparaginase I (NCBI) compare
b1505 ydeT +0.8 0.5 hypothetical protein (RefSeq) compare
b4441 glmY +0.8 1.7 sRNA activator of glmS mRNA (RefSeq) compare
b4679 yohP +0.7 1.9 expressed protein (RefSeq) compare
b0720 gltA +0.7 3.8 citrate synthase (NCBI) compare
b3248 yhdE +0.7 1.9 Maf-like protein (NCBI) compare
b3397 nudE +0.7 5.4 ADP-ribose diphosphatase (NCBI) compare
b2567 rnc +0.7 0.8 ribonuclease III (NCBI) compare
b4450 arcZ +0.7 2.4 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b4590 ybfK +0.7 0.8 hypothetical protein (RefSeq) compare
b1165 ymgA +0.7 0.6 hypothetical protein (NCBI) compare
b3546 yhjW +0.7 6.3 orf, hypothetical protein (VIMSS) compare


Specific Phenotypes

For 40 genes in this experiment

For stress Trimethoprim in Escherichia coli BW25113

For stress Trimethoprim across organisms