Experiment set12IT042 for Escherichia coli BW25113

Compare to:

LB with Trimethoprim 0.00125 mM

200 most detrimental genes:

  gene name fitness t score description  
b0685 ybfE +6.7 25.3 orf, hypothetical protein (VIMSS) compare
b3390 aroK +5.6 24.3 shikimate kinase I (NCBI) compare
b1530 marR +4.9 6.7 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b3860 dsbA +4.8 37.0 periplasmic protein disulfide isomerase I (NCBI) compare
b3162 deaD +4.8 22.4 inducible ATP-independent RNA helicase (VIMSS) compare
b0464 acrR +4.5 34.7 DNA-binding transcriptional repressor (NCBI) compare
b4685 yrbN +4.3 11.1 expressed protein (RefSeq) compare
b2814 metZ +3.8 13.3 tRNA-Met (NCBI) compare
b3493 pitA +3.8 30.2 phosphate transporter, low-affinity (NCBI) compare
b4414 tff +3.7 13.7 unknown RNA (NCBI) compare
b0051 ksgA +3.6 15.3 dimethyladenosine transferase (NCBI) compare
b0439 lon +3.4 22.4 DNA-binding ATP-dependent protease La (NCBI) compare
b1185 dsbB +3.2 9.8 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b2620 smpB +3.0 6.6 SsrA-binding protein (NCBI) compare
b3934 cytR +2.9 25.1 DNA-binding transcriptional dual regulator (NCBI) compare
b2531 iscR +2.9 7.6 DNA-binding transcriptional repressor (NCBI) compare
b4042 dgkA +2.9 9.9 diacylglycerol kinase (NCBI) compare
b3261 fis +2.8 8.0 DNA-binding protein Fis (NCBI) compare
b2684 mprA +2.8 11.0 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b1804 rnd +2.8 12.7 ribonuclease D (NCBI) compare
b4146 yjeK +2.7 10.8 predicted lysine aminomutase (NCBI) compare
b2948 yqgE +2.6 3.9 orf, hypothetical protein (VIMSS) compare
b3247 cafA +2.6 21.7 bundles of cytoplasmic filaments (VIMSS) compare
b3398 yrfF +2.5 6.6 predicted inner membrane protein (NCBI) compare
b2688 gshA +2.4 14.9 glutamate--cysteine ligase (NCBI) compare
b4203 rplI +2.4 12.4 50S ribosomal protein L9 (NCBI) compare
b4005 purD +2.4 20.5 phosphoribosylamine--glycine ligase (NCBI) compare
b0564 appY +2.3 2.2 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b3761 trpT +2.3 7.7 tRNA-Trp (NCBI) compare
b2312 purF +2.2 15.9 amidophosphoribosyltransferase (NCBI) compare
b3673 emrD +2.2 18.5 2-module integral membrane pump; multidrug resistance (VIMSS) compare
b2040 rfbD +2.1 17.1 dTDP-4-dehydrorhamnose reductase subunit, NAD(P)-binding, of dTDP-L-rhamnose synthase (NCBI) compare
b2330 yfcB +2.1 4.9 putative adenine-specific methylase (VIMSS) compare
b1630 rsxD +2.1 10.7 electron transport complex protein RnfD (NCBI) compare
b0746 valZ +2.0 8.1 tRNA-Val (NCBI) compare
b1632 rsxE +2.0 11.6 NADH-ubiquinone oxidoreductase (NCBI) compare
b4375 prfC +2.0 10.0 peptide chain release factor 3 (NCBI) compare
b2557 purL +2.0 15.1 phosphoribosylformylglycinamidine synthase (NCBI) compare
b0955 ycbZ +1.8 12.1 predicted peptidase (NCBI) compare
b2218 rcsC +1.8 13.4 hybrid sensory kinase in two-component regulatory system with RcsB and YojN (RefSeq) compare
b3871 bipA +1.8 14.4 GTP-binding protein (NCBI) compare
b2570 rseC +1.7 11.0 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b1628 rsxB +1.7 5.3 electron transport complex protein RnfB (NCBI) compare
b2066 udk +1.7 6.8 uridine/cytidine kinase (VIMSS) compare
b2216 rcsD +1.7 9.1 phosphotransfer intermediate protein in two-component regulatory system with RcsBC (NCBI) compare
b2041 rfbB +1.7 14.3 dTDP-glucose 4,6 dehydratase, NAD(P)-binding (NCBI) compare
b2499 purM +1.7 9.3 phosphoribosylaminoimidazole synthetase (NCBI) compare
b2214 yojL +1.7 12.4 predicted thiamine biosynthesis lipoprotein (NCBI) compare
b1109 ndh +1.7 9.1 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b2476 purC +1.6 4.7 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b3190 yrbA +1.6 3.5 orf, hypothetical protein (VIMSS) compare
b4000 hupA +1.6 6.4 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b3785 wzzE +1.5 11.1 putative transport protein (VIMSS) compare
b3129 sohA +1.5 4.6 predicted regulator (NCBI) compare
b4202 rpsR +1.5 3.4 30S ribosomal protein S18 (NCBI) compare
b3181 greA +1.5 8.5 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1048 mdoG +1.5 9.1 glucan biosynthesis protein, periplasmic (NCBI) compare
b3229 sspA +1.5 5.6 stringent starvation protein A (NCBI) compare
b2038 rfbC +1.5 9.3 dTDP-4-deoxyrhamnose-3,5-epimerase (NCBI) compare
b1629 rsxC +1.5 8.5 electron transport complex protein RnfC (NCBI) compare
b1984 asnW +1.4 3.1 tRNA-Asn (NCBI) compare
b4480 hdfR +1.4 7.0 transcriptional regulator HdfR (NCBI) compare
b1049 mdoH +1.4 11.0 glucosyltransferase MdoH (NCBI) compare
b2765 ygcM +1.4 6.6 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) (NCBI) compare
b0523 purE +1.4 3.6 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b2947 gshB +1.4 8.1 glutathione synthetase (NCBI) compare
b1627 rsxA +1.4 5.9 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b0122 yacC +1.4 7.4 orf, hypothetical protein (VIMSS) compare
b0200 gmhB +1.4 3.1 hypothetical protein (NCBI) compare
b2749 ygbE +1.4 7.9 conserved inner membrane protein (NCBI) compare
b2618 b2618 +1.4 3.8 orf, hypothetical protein (VIMSS) compare
b0449 mdlB +1.4 10.6 fused predicted multidrug transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1977 asnT +1.3 3.6 tRNA-Asn (NCBI) compare
b3781 trxA +1.3 5.6 thioredoxin 1 (VIMSS) compare
b3228 sspB +1.3 5.2 ClpXP protease specificity-enhancing factor (NCBI) compare
b2938 speA +1.3 9.9 arginine decarboxylase (NCBI) compare
b4391 yjjK +1.3 8.9 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b3636 rpmG +1.2 2.4 50S ribosomal protein L33 (NCBI) compare
b2937 speB +1.2 7.9 agmatinase (NCBI) compare
b2796 sdaC +1.2 9.9 predicted serine transporter (NCBI) compare
b3500 gor +1.2 3.1 glutathione reductase (NCBI) compare
b3764 yifE +1.2 3.3 hypothetical protein (NCBI) compare
b0448 mdlA +1.2 9.5 fused predicted multidrug transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b0145 dksA +1.2 3.0 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b3204 ptsN +1.1 6.1 sugar-specific enzyme IIA component of PTS (NCBI) compare
b3389 aroB +1.1 4.3 3-dehydroquinate synthase (NCBI) compare
b3166 truB +1.1 6.8 tRNA pseudouridine synthase B (NCBI) compare
b3649 rpoZ +1.1 2.1 DNA-directed RNA polymerase subunit omega (NCBI) compare
b3545 proK +1.1 3.4 tRNA-Pro (NCBI) compare
b1642 slyA +1.1 1.6 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b1758 b1758 +1.1 1.8 putative cytochrome oxidase (VIMSS) compare
b4392 slt +1.1 9.7 soluble lytic murein transglycosylase (VIMSS) compare
b2911 ssrS +1.1 1.4 6S regulatory RNA (NCBI) compare
b4155 yjeA +1.1 1.6 putative lysyl-tRNA synthetase (VIMSS) compare
b1766 sppA +1.1 7.4 protease IV (signal peptide peptidase) (NCBI) conserved
b1606 folM +1.1 7.1 short chain dehydrogenase (NCBI) compare
b3071 yqjI +1.1 4.1 predicted transcriptional regulator (NCBI) compare
b1831 proQ +1.1 4.0 putative solute/DNA competence effector (NCBI) compare
b1476 fdnI +1.1 1.6 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b0059 hepA +1.1 9.2 ATP-dependent helicase HepA (NCBI) compare
b2502 ppx +1.0 7.0 exopolyphosphatase (NCBI) compare
b2042 galF +1.0 6.8 predicted subunit with GalU (NCBI) compare
b2319 usg +1.0 2.6 hypothetical protein (NCBI) compare
b1573 ydfC +1.0 1.8 Qin prophage; predicted protein (RefSeq) compare
b0471 ybaB +1.0 3.7 hypothetical protein (NCBI) compare
b3466 yhhL +1.0 4.4 conserved inner membrane protein (NCBI) compare
b2812 ygdL +1.0 6.1 hypothetical protein (NCBI) compare
b4618 tisB +1.0 3.2 lexA-regulated toxic peptide (RefSeq) compare
b3465 yhhF +1.0 6.1 predicted methyltransferase (NCBI) compare
b4445 omrB +1.0 2.9 sRNA down regulates OM proteins; positively regulated by OmpR/EnvZ; binds Hfq (RefSeq) compare
b3706 trmE +1.0 4.8 tRNA modification GTPase (NCBI) compare
b1222 narX +1.0 2.9 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b1989 asnV +1.0 3.2 tRNA-Asn (NCBI) compare
b3741 gidA +1.0 5.1 glucose-inhibited division protein A (NCBI) compare
b0014 dnaK +1.0 2.3 molecular chaperone DnaK (NCBI) compare
b1292 sapC +1.0 2.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0851 nfsA +0.9 4.7 nitroreductase A, NADPH-dependent, FMN-dependent (NCBI) compare
b2318 truA +0.9 3.2 tRNA pseudouridine synthase A (NCBI) compare
b1290 sapF +0.9 1.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0889 lrp +0.9 2.2 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b2962 yggX +0.9 4.3 hypothetical protein (NCBI) compare
b2340 sixA +0.9 4.3 phosphohistidine phosphatase (NCBI) compare
b3258 panF +0.9 6.9 sodium/pantothenate symporter (VIMSS) compare
b3257 yhdT +0.9 4.7 conserved inner membrane protein (NCBI) compare
b4431 rprA +0.9 1.8 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b4592 yccB +0.9 1.4 hypothetical protein (RefSeq) compare
b1259 yciG +0.8 1.9 orf, hypothetical protein (VIMSS) compare
b2682 ygaZ +0.8 2.3 predicted transporter (NCBI) compare
b1824 yobF +0.8 2.5 hypothetical protein (NCBI) compare
b1362 rzpR +0.8 1.7 putative Rac prophage endopeptidase (VIMSS) compare
b3244 tldD +0.8 6.4 predicted peptidase (NCBI) compare
b1961 dcm +0.8 1.7 DNA cytosine methylase (NCBI) compare
b0082 mraW +0.8 2.7 S-adenosyl-methyltransferase (NCBI) compare
b0401 brnQ +0.8 6.0 predicted branched chain amino acid transporter (LIV-II) (NCBI) compare
b0797 rhlE +0.8 4.4 RNA helicase (NCBI) compare
b1427 rimL +0.8 2.5 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b3146 yraL +0.8 4.5 predicted methyltransferase (NCBI) compare
b3164 pnp +0.8 1.8 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b2567 rnc +0.8 1.0 ribonuclease III (NCBI) compare
b0114 aceE +0.8 0.9 pyruvate dehydrogenase subunit E1 (NCBI) compare
b4255 yjgD +0.8 3.7 hypothetical protein (NCBI) compare
b2313 cvpA +0.8 2.6 membrane protein required for colicin V production (NCBI) compare
b1248 b1248 +0.8 2.3 orf, hypothetical protein (VIMSS) compare
b4235 pmbA +0.8 6.1 predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 (NCBI) compare
b3774 ilvC +0.8 6.2 ketol-acid reductoisomerase (NCBI) compare
b0242 proB +0.8 2.9 gamma-glutamyl kinase (NCBI) compare
b0418 pgpA +0.8 3.3 phosphatidylglycerophosphatase A (NCBI) compare
b1871 yecP +0.8 3.5 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b2285 nuoE +0.8 2.1 NADH dehydrogenase subunit E (NCBI) compare
b0435 bolA +0.8 3.7 possible regulator of murein genes (VIMSS) compare
b2113 mrp +0.8 4.6 putative ATPase (VIMSS) compare
b3778 rep +0.8 5.2 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b2587 kgtP +0.8 4.1 alpha-ketoglutarate transporter (NCBI) compare
b2946 yggJ +0.8 3.7 orf, hypothetical protein (VIMSS) compare
b3554 yiaF +0.7 5.8 orf, hypothetical protein (VIMSS) compare
b3149 yraO +0.7 4.2 DnaA initiator-associating factor for replication initiation (NCBI) compare
b0357 yaiN +0.7 1.1 putative alpha helix chain (VIMSS) compare
b4370 leuQ +0.7 2.3 tRNA-Leu (NCBI) compare
b0009 mog +0.7 4.4 molybdenum cofactor biosynthesis protein (NCBI) compare
b4418 sraB +0.7 1.8 unknown RNA (NCBI) compare
b2450 yffS +0.7 1.9 CPZ-55 prophage; predicted protein (RefSeq) compare
b4214 cysQ +0.7 5.7 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b4077 gltP +0.7 4.9 glutamate/aspartate:proton symporter (NCBI) compare
b3672 ivbL +0.7 2.9 ilvB operon leader peptide (NCBI) compare
b1217 chaB +0.7 1.3 cation transport regulator (NCBI) compare
b1302 puuE +0.7 2.1 GABA aminotransferase, PLP-dependent (NCBI) compare
b0183 rnhB +0.7 1.8 ribonuclease HII (NCBI) compare
b0710 ybgI +0.7 3.7 conserved metal-binding protein (NCBI) compare
b3979 thrT +0.7 0.7 tRNA-Thr (NCBI) compare
b0440 hupB +0.7 3.5 HU, DNA-binding transcriptional regulator, beta subunit (NCBI) compare
b1512 lsrR +0.7 2.7 lsr operon transcriptional repressor (NCBI) compare
b3401 yrfI +0.7 4.5 orf, hypothetical protein (VIMSS) compare
b0393 rdgC +0.7 4.9 recombination associated protein (NCBI) compare
b2210 mqo +0.7 5.2 malate:quinone oxidoreductase (NCBI) compare
b0118 acnB +0.7 0.9 aconitate hydratase (NCBI) compare
b0426 yajQ +0.7 3.4 orf, hypothetical protein (VIMSS) compare
b3766 ilvL +0.7 2.2 ilvG operon leader peptide (NCBI) compare
b4139 aspA +0.7 5.3 aspartate ammonia-lyase (aspartase) (VIMSS) compare
b2701 mltB +0.7 3.7 membrane-bound lytic murein transglycosylase B (NCBI) compare
b1334 fnr +0.7 1.8 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b4201 priB +0.7 1.2 primosomal replication protein N (NCBI) compare
b3610 grxC +0.7 4.2 glutaredoxin 3 (NCBI) compare
b2303 folX +0.7 4.4 D-erythro-7,8-dihydroneopterin triphosphate 2'-epimerase and dihydroneopterin aldolase (NCBI) compare
b1691 ydiN +0.7 2.2 predicted transporter (RefSeq) compare
b3789 rffH +0.6 2.4 glucose-1-phosphate thymidylyltransferase (NCBI) compare
b4078 yjcO +0.6 5.1 hypothetical protein (NCBI) compare
b3816 corA +0.6 4.5 magnesium/nickel/cobalt transporter (NCBI) compare
b2889 idi +0.6 3.1 isopentenyl-diphosphate delta-isomerase (NCBI) compare
b2017 yefM +0.6 1.8 orf, hypothetical protein (VIMSS) compare
b2580 ung +0.6 4.4 uracil-DNA glycosylase (NCBI) compare
b2810 csdA +0.6 3.7 cysteine sulfinate desulfinase (NCBI) compare
b1777 b1777 +0.6 1.4 orf, hypothetical protein (VIMSS) compare
b4025 pgi +0.6 4.1 glucose-6-phosphate isomerase (NCBI) compare
b2399 yfeD +0.6 2.7 predicted DNA-binding transcriptional regulator (RefSeq) compare
b3248 yhdE +0.6 1.7 Maf-like protein (NCBI) compare
b2564 pdxJ +0.6 1.7 pyridoxal phosphate biosynthetic protein (NCBI) compare
b4179 vacB +0.6 3.3 putative enzyme (VIMSS) compare
b2236 yfaE +0.6 1.6 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b3739 atpI +0.6 2.5 membrane-bound ATP synthase, dispensable protein, affects expression of atpB (VIMSS) compare
b2707 srlR +0.6 2.8 DNA-bindng transcriptional repressor (NCBI) compare


Specific Phenotypes

For 37 genes in this experiment

For stress Trimethoprim in Escherichia coli BW25113

For stress Trimethoprim across organisms