Experiment set12IT038 for Pseudomonas putida KT2440

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1,5-Pentanediol carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + 1,5-Pentanediol (10 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Putida_ML5_JBEI, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=700rpm
By: Matthew Incha on 12-Feb-19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 13 genes in this experiment

For carbon source 1,5-Pentanediol in Pseudomonas putida KT2440

For carbon source 1,5-Pentanediol across organisms

SEED Subsystems

Subsystem #Specific
Molybdenum cofactor biosynthesis 3
N-heterocyclic aromatic compound degradation 2
Ammonia assimilation 1
Transport of Molybdenum 1
Universal stress protein family 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
glutarate degradation 2 2 1
molybdopterin biosynthesis 6 4 1
2-deoxy-D-ribose degradation II 8 4 1
photorespiration I 9 6 1