Experiment set12IT030 for Escherichia coli BW25113

Compare to:

LB with Nalidixic acid sodium salt 0.0313 mM

200 most important genes:

  gene name fitness t score description  
b1855 lpxM -5.5 -3.8 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b3035 tolC -4.9 -5.9 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b0957 ompA -4.3 -4.2 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b2346 vacJ -4.3 -9.1 predicted lipoprotein (NCBI) compare
b0178 hlpA -4.2 -6.4 periplasmic chaperone (NCBI) compare
b2512 yfgL -4.1 -5.6 protein assembly complex, lipoprotein component (NCBI) compare
b0912 ihfB -3.9 -5.8 integration host factor subunit beta (NCBI) compare
b3163 nlpI -3.8 -5.2 hypothetical protein (NCBI) compare
b4201 priB -3.6 -4.1 primosomal replication protein N (NCBI) compare
b1830 prc -3.5 -3.4 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b2174 b2174 -3.4 -10.9 orf, hypothetical protein (VIMSS) compare
b1192 ldcA -3.4 -6.8 L,D-carboxypeptidase A (NCBI) compare
b3935 priA -3.4 -3.9 primosome assembly protein PriA (NCBI) compare
b2616 recN -3.3 -13.1 recombination and repair protein (NCBI) conserved
b3792 wzxE -3.2 -4.4 O-antigen translocase (NCBI) compare
b0053 surA -3.1 -6.3 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b2494 yfgC -3.1 -10.2 predicted peptidase (NCBI) compare
b1765 ydjA -3.0 -1.5 predicted oxidoreductase (NCBI) compare
b3199 yrbK -3.0 -4.0 hypothetical protein (NCBI) compare
b2617 smpA -2.9 -4.4 small membrane protein A (VIMSS) compare
b2317 dedA -2.8 -7.2 conserved inner membrane protein (NCBI) compare
b3195 yrbF -2.8 -13.6 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b1677 lpp -2.8 -3.2 murein lipoprotein (NCBI) compare
b3192 yrbC -2.7 -12.5 predicted ABC-type organic solvent transporter (NCBI) compare
b3194 yrbE -2.6 -14.4 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b3621 rfaC -2.6 -3.1 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3193 yrbD -2.5 -11.6 predicted ABC-type organic solvent transporter (NCBI) compare
b0462 acrB -2.5 -18.2 multidrug efflux system protein (NCBI) compare
b1236 galU -2.5 -2.4 glucose-1-phosphate uridylyltransferase (NCBI) compare
b0222 lpcA -2.5 -2.9 phosphoheptose isomerase (NCBI) compare
b1882 cheY -2.5 -1.2 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b4481 rffT -2.4 -8.3 4-alpha-L-fucosyltransferase (NCBI) compare
b0463 acrA -2.4 -12.8 multidrug efflux system (NCBI) compare
b3631 rfaG -2.4 -12.7 glucosyltransferase I (NCBI) compare
b3613 yibP -2.3 -2.2 putative membrane protein (VIMSS) compare
b3191 yrbB -2.3 -9.8 orf, hypothetical protein (VIMSS) compare
b0149 mrcB -2.2 -14.1 penicillin-binding protein 1b (NCBI) compare
b0688 pgm -2.2 -3.3 phosphoglucomutase (NCBI) compare
b3619 rfaD -2.1 -2.9 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3052 rfaE -2.1 -5.3 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3620 rfaF -2.1 -5.7 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b2509 xseA -2.1 -9.8 exodeoxyribonuclease VII large subunit (NCBI) conserved
b3790 rffC -2.1 -6.4 TDP-fucosamine acetyltransferase (RefSeq) compare
b1261 trpB -2.0 -3.7 tryptophan synthase subunit beta (NCBI) compare
b0759 galE -1.9 -6.3 UDP-galactose-4-epimerase (VIMSS) compare
b0145 dksA -1.9 -4.0 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b0049 apaH -1.9 -3.5 diadenosinetetraphosphatase (NCBI) compare
b0200 gmhB -1.9 -1.3 hypothetical protein (NCBI) compare
b2572 rseA -1.8 -3.7 anti-sigma factor (NCBI) compare
b3026 qseC -1.8 -2.1 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b4676 yoaK -1.8 -1.2 expressed protein (RefSeq) compare
b1861 ruvA -1.7 -1.2 Holliday junction DNA helicase motor protein (NCBI) compare
b3791 rffA -1.7 -8.0 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b1279 yciS -1.6 -1.6 conserved inner membrane protein (NCBI) compare
b4451 ryhB -1.5 -2.7 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b1604 ydgH -1.5 -4.9 hypothetical protein (NCBI) compare
b0407 yajC -1.5 -3.1 preprotein translocase subunit YajC (NCBI) compare
b3183 obgE -1.5 -1.6 GTPase involved in cell partioning and DNA repair (NCBI) compare
b2188 yejM -1.5 -8.0 predicted hydrolase, inner membrane (NCBI) compare
b3632 rfaQ -1.5 -12.2 lipopolysaccharide core biosynthesis protein (NCBI) compare
b4701 sokX -1.4 -1.2 sok-related sRNA, function unknown (RefSeq) compare
b4698 mgrR -1.4 -3.4 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b1863 ruvC -1.4 -2.1 Holliday junction resolvase (NCBI) compare
b0232 yafN -1.4 -0.9 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b3261 fis -1.4 -1.3 DNA-binding protein Fis (NCBI) compare
b3842 rfaH -1.4 -4.9 transcriptional activator RfaH (NCBI) compare
b1961 dcm -1.4 -1.9 DNA cytosine methylase (NCBI) compare
b0683 fur -1.4 -1.3 ferric uptake regulator (NCBI) compare
b3652 recG -1.3 -7.7 ATP-dependent DNA helicase (NCBI) compare
b0450 glnK -1.3 -3.2 nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB (NCBI) compare
b3628 rfaB -1.3 -6.9 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b0740 tolB -1.3 -3.9 translocation protein TolB precursor (NCBI) compare
b2814 metZ -1.3 -1.8 tRNA-Met (NCBI) compare
b1531 marA -1.2 -2.6 multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS) compare
b2175 spr -1.2 -3.0 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b3609 secB -1.2 -3.3 export protein SecB (NCBI) compare
b0739 tolA -1.2 -2.2 cell envelope integrity inner membrane protein TolA (NCBI) compare
b3627 rfaI -1.2 -6.6 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b3785 wzzE -1.2 -7.7 putative transport protein (VIMSS) compare
b0738 tolR -1.2 -1.5 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b2282 nuoH -1.1 -2.5 NADH dehydrogenase subunit H (NCBI) compare
b2597 yfiA -1.1 -2.2 cold shock protein associated with 30S ribosomal subunit (NCBI) compare
b0436 tig -1.1 -5.8 trigger factor (NCBI) compare
b0741 pal -1.1 -2.7 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b1317 ycjU -1.1 -3.1 predicted beta-phosphoglucomutase (NCBI) compare
b1712 ihfA -1.1 -0.7 integration host factor subunit alpha (NCBI) compare
b1176 minC -1.1 -2.9 septum formation inhibitor (NCBI) compare
b2279 nuoK -1.1 -1.3 NADH dehydrogenase subunit K (NCBI) compare
b3630 rfaP -1.1 -6.1 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b3784 rfe -1.1 -8.5 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b1205 ychH -1.1 -1.6 predicted inner membrane protein (NCBI) compare
b1831 proQ -1.1 -2.0 putative solute/DNA competence effector (NCBI) compare
b1739 osmE -1.1 -2.8 DNA-binding transcriptional activator (NCBI) compare
b0819 ybiS -1.0 -5.5 hypothetical protein (NCBI) compare
b2830 nudH -1.0 -1.1 dinucleoside polyphosphate hydrolase (NCBI) compare
b4596 yciZ -1.0 -2.2 hypothetical protein (RefSeq) compare
b2215 ompC -1.0 -5.9 outer membrane porin protein C (NCBI) compare
b3443 yrhA -1.0 -1.6 orf, hypothetical protein (VIMSS) compare
b2642 yfjW -1.0 -1.1 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b3811 xerC -1.0 -1.3 site-specific tyrosine recombinase XerC (NCBI) compare
b1105 ycfM -1.0 -3.4 predicted outer membrane lipoprotein (NCBI) compare
b1175 minD -1.0 -3.8 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b1195 ymgE -1.0 -2.9 predicted inner membrane protein (NCBI) compare
b2288 nuoA -0.9 -2.6 NADH dehydrogenase I chain A (VIMSS) compare
b1109 ndh -0.9 -3.1 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b4163 glyV -0.9 -1.3 tRNA-Gly (NCBI) compare
b0018 mokC -0.9 -1.4 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b4546 ypeB -0.8 -1.3 hypothetical protein (NCBI) compare
b1671 ydhX -0.8 -1.6 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b1185 dsbB -0.8 -2.2 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b0877 ybjX -0.8 -3.6 hypothetical protein (NCBI) compare
b3786 rffE -0.8 -6.1 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b0890 ftsK -0.8 -2.1 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b1235 rssB -0.8 -4.0 response regulator of RpoS (NCBI) compare
b2571 rseB -0.8 -3.6 periplasmic negative regulator of sigmaE (NCBI) compare
b3396 mrcA -0.8 -7.0 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b2325 yfcL -0.8 -2.7 hypothetical protein (NCBI) compare
b1304 pspA -0.8 -2.5 regulatory protein for phage-shock-protein operon (NCBI) compare
b3860 dsbA -0.8 -5.0 periplasmic protein disulfide isomerase I (NCBI) compare
b1361 ydaW -0.8 -0.5 orf, hypothetical protein (VIMSS) compare
b3147 yraM -0.8 -6.0 hypothetical protein (NCBI) compare
b3933 ftsN -0.8 -4.8 essential cell division protein (NCBI) compare
b0161 degP -0.7 -4.5 serine endoprotease (protease Do), membrane-associated (NCBI) compare
b2058 wcaB -0.7 -1.0 predicted acyl transferase (NCBI) compare
b1658 purR -0.7 -3.6 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b0357 yaiN -0.7 -1.6 putative alpha helix chain (VIMSS) compare
b3250 mreC -0.7 -1.3 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b3649 rpoZ -0.7 -2.2 DNA-directed RNA polymerase subunit omega (NCBI) compare
b0471 ybaB -0.7 -2.5 hypothetical protein (NCBI) compare
b4522 yciX_1 -0.7 -1.5 hypothetical protein (NCBI) compare
b0097 yacA -0.7 -1.5 orf, hypothetical protein (VIMSS) compare
b2209 eco -0.7 -1.6 ecotin precursor (NCBI) compare
b4702 mgtL -0.7 -1.3 regulatory leader peptide for mgtA (NCBI) compare
b3728 pstS -0.7 -1.6 phosphate transporter subunit (NCBI) compare
b3787 rffD -0.7 -4.6 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b4402 yjjY -0.7 -0.9 hypothetical protein (NCBI) compare
b1618 uidR -0.7 -2.4 DNA-binding transcriptional repressor (NCBI) compare
b0676 nagC -0.7 -4.4 DNA-binding transcriptional dual regulator, repressor of N-acetylglucosamine (NCBI) compare
b2064 asmA -0.7 -3.8 predicted assembly protein (NCBI) compare
b2892 recJ -0.7 -4.3 ssDNA exonuclease, 5' --> 3'-specific (NCBI) compare
b2287 nuoB -0.7 -1.8 NADH dehydrogenase subunit B (NCBI) compare
b3726 pstA -0.7 -2.8 phosphate transporter subunit (NCBI) compare
b4458 oxyS -0.7 -1.3 global regulatory RNA (NCBI) compare
b2569 lepA -0.7 -2.2 GTP-binding protein LepA (NCBI) compare
b2553 glnB -0.7 -2.9 regulatory protein P-II for glutamine synthetase (NCBI) compare
b2822 recC -0.7 -0.9 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b2286 nuoC -0.7 -2.9 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b1429 tehA -0.7 -0.9 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b4480 hdfR -0.7 -3.2 transcriptional regulator HdfR (NCBI) compare
b3778 rep -0.7 -3.9 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b4396 rob -0.6 -4.6 DNA-binding transcriptional activator (NCBI) compare
b2576 srmB -0.6 -1.9 ATP-dependent RNA helicase (NCBI) compare
b1284 yciT -0.6 -2.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1278 pgpB -0.6 -1.3 phosphatidylglycerophosphatase B (NCBI) compare
b0430 cyoC -0.6 -2.4 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b4577 sgrS -0.6 -1.6 small antisense RNA (NCBI) compare
b1266 yciV -0.6 -2.0 hypothetical protein (NCBI) compare
b2011 sbcB -0.6 -3.5 exonuclease I (NCBI) compare
b2819 recD -0.6 -3.3 exonuclease V (RecBCD complex), alpha chain (NCBI) compare
b1763 topB -0.6 -1.7 DNA topoisomerase III (NCBI) compare
b1524 yneH -0.6 -0.7 predicted glutaminase (NCBI) compare
b4535 yniD -0.6 -0.7 predicted protein (RefSeq) compare
b3387 dam -0.6 -4.8 DNA adenine methylase (NCBI) compare
b1778 yeaA -0.6 -1.1 methionine sulfoxide reductase B (NCBI) compare
b2650 ypjC -0.6 -0.4 hypothetical protein (RefSeq) compare
b1438 ydcQ -0.6 -1.8 predicted DNA-binding transcriptional regulator (RefSeq) compare
b4100 phnH -0.6 -2.5 carbon-phosphorus lyase complex subunit (NCBI) compare
b3389 aroB -0.6 -4.3 3-dehydroquinate synthase (NCBI) compare
b0406 tgt -0.6 -2.8 queuine tRNA-ribosyltransferase (NCBI) compare
b2363 yfdT -0.6 -1.2 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b1693 aroD -0.6 -2.9 3-dehydroquinate dehydratase (NCBI) compare
b2476 purC -0.6 -2.1 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b1569 dicC -0.6 -1.7 Qin prophage; DNA-binding transcriptional regulator for DicB (NCBI) compare
b4573 insZ -0.6 -1.9 no description compare
b3839 tatC -0.6 -3.1 TatABCE protein translocation system subunit (NCBI) compare
b2221 atoD -0.6 -2.2 acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI) compare
b1074 flgC -0.6 -0.8 flagellar basal-body rod protein C (NCBI) compare
b1643 ydhI -0.6 -2.0 predicted inner membrane protein (NCBI) compare
b1227 narI -0.6 -2.3 nitrate reductase 1, gamma (cytochrome b(NR)) subunit (NCBI) compare
b0737 tolQ -0.6 -1.0 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3931 hslU -0.6 -4.3 ATP-dependent protease ATP-binding subunit (NCBI) compare
b1757 b1757 -0.6 -2.5 putative thiosulfate sulfur transferase (VIMSS) compare
b0441 ppiD -0.6 -4.4 peptidyl-prolyl cis-trans isomerase (rotamase D) (NCBI) compare
b4695 ykgT -0.6 -0.9 no description compare
b0843 ybjH -0.5 -1.0 hypothetical protein (NCBI) compare
b3094 exuR -0.5 -2.3 negative regulator of exu regulon, exuT, uxaAC, and uxuB (VIMSS) compare
b3764 yifE -0.5 -2.4 hypothetical protein (NCBI) compare
b3813 uvrD -0.5 -4.3 DNA-dependent ATPase I and helicase II (NCBI) compare
b1370 insH-5 -0.5 -1.2 Rac prophage; IS5 transposase and trans-activator (NCBI) compare
b4062 soxS -0.5 -2.3 DNA-binding transcriptional dual regulator (NCBI) compare
b0853 ybjN -0.5 -2.1 predicted oxidoreductase (NCBI) compare
b1612 fumA -0.5 -1.8 fumarate hydratase (fumarase A), aerobic Class I (NCBI) compare
b0550 rusA -0.5 -1.4 DLP12 prophage; endonuclease RUS (NCBI) compare
b1259 yciG -0.5 -0.9 orf, hypothetical protein (VIMSS) compare
b2998 yghW -0.5 -2.2 hypothetical protein (NCBI) compare
b0611 rna -0.5 -2.5 ribonuclease I (NCBI) compare
b0484 copA -0.5 -4.0 copper transporter (NCBI) compare
b2424 cysU -0.5 -1.1 sulfate/thiosulfate transporter subunit (NCBI) compare
b2584 yfiQ -0.5 -4.0 fused predicted acyl-CoA synthetase: NAD(P)-binding subunit/ATP-binding subunit (NCBI) compare
b3149 yraO -0.5 -2.5 DnaA initiator-associating factor for replication initiation (NCBI) compare


Specific Phenotypes

For 4 genes in this experiment

For stress Nalidixic acid sodium salt in Escherichia coli BW25113

For stress Nalidixic acid sodium salt across organisms