Experiment set12IT028 for Pseudomonas putida KT2440
L-Glutamic acid monopotassium salt monohydrate carbon source
Group: carbon sourceMedia: MOPS minimal media_noCarbon + L-Glutamic acid monopotassium salt monohydrate (10 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Putida_ML5_JBEI, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=700rpm
By: Matthew Incha on 12-Feb-19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 7 genes in this experiment
For carbon source L-Glutamic acid monopotassium salt monohydrate in Pseudomonas putida KT2440
For carbon source L-Glutamic acid monopotassium salt monohydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Pyrimidine utilization | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| urea degradation I | 3 | 2 | 2 |
| cyanuric acid degradation II | 5 | 3 | 2 |
| cyanuric acid degradation I | 5 | 2 | 2 |
| L-citrulline degradation | 3 | 3 | 1 |
| cyanate degradation | 3 | 2 | 1 |
| superpathway of allantoin degradation in yeast | 6 | 3 | 2 |
| L-arginine degradation V (arginine deiminase pathway) | 4 | 4 | 1 |
| superpathway of atrazine degradation | 8 | 3 | 2 |
| uracil degradation III | 5 | 3 | 1 |
| allantoin degradation IV (anaerobic) | 9 | 2 | 1 |