Experiment set12IT028 for Escherichia coli BW25113

Compare to:

LB with Trimethoprim 0.0025 mM

200 most detrimental genes:

  gene name fitness t score description  
b3493 pitA +2.5 19.1 phosphate transporter, low-affinity (NCBI) compare
b3390 aroK +2.2 6.9 shikimate kinase I (NCBI) compare
b2814 metZ +2.2 6.1 tRNA-Met (NCBI) compare
b0439 lon +1.8 9.8 DNA-binding ATP-dependent protease La (NCBI) compare
b2830 nudH +1.6 3.1 dinucleoside polyphosphate hydrolase (NCBI) compare
b0014 dnaK +1.6 3.2 molecular chaperone DnaK (NCBI) compare
b4480 hdfR +1.4 9.4 transcriptional regulator HdfR (NCBI) compare
b2318 truA +1.4 4.9 tRNA pseudouridine synthase A (NCBI) compare
b1048 mdoG +1.4 8.4 glucan biosynthesis protein, periplasmic (NCBI) compare
b1847 yebF +1.4 2.5 predicted protein (RefSeq) compare
b3261 fis +1.4 3.6 DNA-binding protein Fis (NCBI) compare
b0931 pncB +1.4 6.7 nicotinate phosphoribosyltransferase (NCBI) compare
b0145 dksA +1.3 4.3 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b0051 ksgA +1.3 6.1 dimethyladenosine transferase (NCBI) compare
b1049 mdoH +1.3 9.7 glucosyltransferase MdoH (NCBI) compare
b3764 yifE +1.3 6.8 hypothetical protein (NCBI) compare
b4005 purD +1.2 9.5 phosphoribosylamine--glycine ligase (NCBI) compare
b0471 ybaB +1.2 5.0 hypothetical protein (NCBI) compare
b0630 lipB +1.2 2.2 protein of lipoate biosynthesis (VIMSS) compare
b2312 purF +1.2 7.3 amidophosphoribosyltransferase (NCBI) compare
b1709 btuD +1.2 1.6 vitamin B12-transporter ATPase (NCBI) compare
b2620 smpB +1.2 1.3 SsrA-binding protein (NCBI) compare
b0464 acrR +1.1 9.7 DNA-binding transcriptional repressor (NCBI) compare
b1976 b1976 +1.1 4.3 orf, hypothetical protein (VIMSS) compare
b2699 recA +1.1 1.5 recombinase A (NCBI) compare
b0523 purE +1.1 5.4 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b4503 yafF +1.0 0.9 no description compare
b3162 deaD +1.0 5.1 inducible ATP-independent RNA helicase (VIMSS) compare
b3247 cafA +1.0 8.5 bundles of cytoplasmic filaments (VIMSS) compare
b2618 b2618 +1.0 2.7 orf, hypothetical protein (VIMSS) compare
b2688 gshA +1.0 7.4 glutamate--cysteine ligase (NCBI) compare
b4000 hupA +1.0 4.2 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b2499 purM +1.0 5.5 phosphoribosylaminoimidazole synthetase (NCBI) compare
b2518 ndk +1.0 3.4 nucleoside diphosphate kinase (NCBI) compare
b0685 ybfE +1.0 2.4 orf, hypothetical protein (VIMSS) compare
b2557 purL +1.0 7.2 phosphoribosylformylglycinamidine synthase (NCBI) compare
b0866 ybjQ +0.9 1.3 hypothetical protein (NCBI) compare
b3204 ptsN +0.9 4.9 sugar-specific enzyme IIA component of PTS (NCBI) compare
b1252 tonB +0.9 3.1 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b4179 vacB +0.9 5.4 putative enzyme (VIMSS) compare
b1942 fliJ +0.9 1.3 flagellar biosynthesis chaperone (NCBI) compare
b0828 iaaA +0.9 4.2 L-asparaginase (NCBI) compare
b3860 dsbA +0.9 6.7 periplasmic protein disulfide isomerase I (NCBI) compare
b1685 ydiH +0.9 1.4 predicted protein (RefSeq) compare
b2340 sixA +0.8 3.8 phosphohistidine phosphatase (NCBI) compare
b1831 proQ +0.8 3.9 putative solute/DNA competence effector (NCBI) compare
b1548 nohA +0.8 2.1 Qin prophage; predicted packaging protein (RefSeq) compare
b3164 pnp +0.8 2.7 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b4551 yheV +0.8 2.0 hypothetical protein (NCBI) compare
b2617 smpA +0.8 2.9 small membrane protein A (VIMSS) compare
b4613 dinQ +0.8 1.9 Damage inducible, function unknown (RefSeq) compare
b1448 yncA +0.8 2.1 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b2390 ypeC +0.8 2.4 hypothetical protein (NCBI) compare
b1358 ydaT +0.7 0.5 Rac prophage; predicted protein (NCBI) compare
b1273 yciN +0.7 1.5 hypothetical protein (NCBI) compare
b0472 recR +0.7 4.6 recombination protein RecR (NCBI) compare
b1632 rsxE +0.7 3.9 NADH-ubiquinone oxidoreductase (NCBI) compare
b1159 mcrA +0.7 1.0 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b3183 obgE +0.7 1.1 GTPase involved in cell partioning and DNA repair (NCBI) compare
b0015 dnaJ +0.7 5.3 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b3389 aroB +0.7 5.4 3-dehydroquinate synthase (NCBI) compare
b0688 pgm +0.7 2.6 phosphoglucomutase (NCBI) compare
b2741 rpoS +0.7 4.0 RNA polymerase sigma factor (NCBI) compare
b2512 yfgL +0.7 4.0 protein assembly complex, lipoprotein component (NCBI) compare
b4592 yccB +0.7 1.2 hypothetical protein (RefSeq) compare
b1882 cheY +0.7 0.7 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1530 marR +0.7 3.7 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b1063 yceB +0.7 2.2 predicted lipoprotein (NCBI) compare
b2947 gshB +0.7 3.4 glutathione synthetase (NCBI) compare
b3005 exbD +0.7 3.8 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1400 paaY +0.7 1.8 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b1357 ydaS +0.7 1.5 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1139 lit +0.7 3.1 e14 prophage; cell death peptidase, inhibitor of T4 late gene expression (NCBI) compare
b4578 insP +0.7 1.7 no description compare
b1628 rsxB +0.7 1.7 electron transport complex protein RnfB (NCBI) compare
b1630 rsxD +0.7 3.7 electron transport complex protein RnfD (NCBI) compare
b0850 ybjC +0.6 2.0 predicted inner membrane protein (NCBI) compare
b1185 dsbB +0.6 1.6 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b1109 ndh +0.6 2.3 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b1426 ydcH +0.6 1.3 orf, hypothetical protein (VIMSS) compare
b2258 b2258 +0.6 1.1 putative transport/receptor protein (VIMSS) compare
b1848 yebG +0.6 1.3 conserved protein regulated by LexA (NCBI) compare
b1527 yneK +0.6 1.5 hypothetical protein (NCBI) compare
b3465 yhhF +0.6 3.8 predicted methyltransferase (NCBI) compare
b3741 gidA +0.6 2.9 glucose-inhibited division protein A (NCBI) compare
b2565 recO +0.6 3.7 DNA repair protein RecO (NCBI) compare
b0706 ybfD +0.6 1.5 hypothetical protein (NCBI) compare
b3119 tdcR +0.6 1.9 threonine dehydratase operon activator protein (VIMSS) compare
b4631 ykgQ +0.6 1.5 no description compare
b4172 hfq +0.6 0.5 RNA-binding protein Hfq (NCBI) compare
b3700 recF +0.6 4.9 recombination protein F (NCBI) compare
b3009 yghB +0.6 3.3 conserved inner membrane protein (NCBI) compare
b0303 ykgI +0.6 0.6 orf, hypothetical protein (VIMSS) compare
b2476 purC +0.6 2.7 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b1823 cspC +0.6 1.2 stress protein, member of the CspA-family (NCBI) compare
b1627 rsxA +0.6 2.1 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b2911 ssrS +0.6 0.7 6S regulatory RNA (NCBI) compare
b1729 ydjN +0.6 2.6 predicted transporter (NCBI) compare
b4388 serB +0.6 2.9 3-phosphoserine phosphatase (NCBI) compare
b2892 recJ +0.6 4.1 ssDNA exonuclease, 5' --> 3'-specific (NCBI) compare
b1837 yebW +0.6 1.5 orf, hypothetical protein (VIMSS) compare
b2421 cysM +0.6 2.2 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b3871 bipA +0.6 4.7 GTP-binding protein (NCBI) compare
b1186 nhaB +0.6 3.2 NhaB sodium/proton transporter (Katherine Huang) compare
b3281 aroE +0.6 4.7 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b1567 intK +0.5 1.4 Qin prophage; predicted protein (RefSeq) compare
b1236 galU +0.5 2.4 glucose-1-phosphate uridylyltransferase (NCBI) compare
b1936 intG +0.5 0.6 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1566 flxA +0.5 1.5 Qin prophage; predicted protein (NCBI) compare
b3673 emrD +0.5 4.4 2-module integral membrane pump; multidrug resistance (VIMSS) compare
b4387 ytjB +0.5 3.2 hypothetical protein (NCBI) compare
b1361 ydaW +0.5 0.4 orf, hypothetical protein (VIMSS) compare
b1951 rcsA +0.5 0.8 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b4101 phnG +0.5 1.9 carbon-phosphorus lyase complex subunit (NCBI) compare
b1079 flgH +0.5 2.7 flagellar L-ring protein precursor H (NCBI) compare
b2414 cysK +0.5 3.4 cysteine synthase A, O-acetylserine sulfhydrolase A subunit (NCBI) compare
b3341 rpsG +0.5 0.8 30S ribosomal protein S7 (NCBI) compare
b0746 valZ +0.5 1.6 tRNA-Val (NCBI) compare
b4041 plsB +0.5 1.4 glycerol-3-phosphate acyltransferase (VIMSS) compare
b1153 ymfQ +0.5 2.5 e14 prophage; conserved protein (NCBI) compare
b0065 yabI +0.5 2.7 conserved inner membrane protein (NCBI) compare
b1766 sppA +0.5 3.4 protease IV (signal peptide peptidase) (NCBI) conserved
b4294 insA-7 +0.5 1.7 KpLE2 phage-like element; IS1 repressor protein InsA (NCBI) compare
b2684 mprA +0.5 2.1 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b2570 rseC +0.5 2.4 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b0888 trxB +0.5 1.9 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b3006 exbB +0.5 2.9 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b4595 yciY +0.5 1.1 hypothetical protein (RefSeq) compare
b4533 ynfO +0.5 1.3 Qin prophage; predicted protein (NCBI) compare
b1148 ymfM +0.5 1.5 e14 prophage; predicted protein (NCBI) compare
b2004 yeeU +0.5 1.8 CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b0527 ybcI +0.5 2.0 conserved inner membrane protein (NCBI) compare
b2582 trxC +0.5 1.0 thioredoxin 2 (NCBI) compare
b1572 ydfB +0.5 0.9 orf, hypothetical protein (VIMSS) compare
b2946 yggJ +0.5 2.5 orf, hypothetical protein (VIMSS) compare
b4191 ulaR +0.5 2.1 DNA-binding transcriptional dual regulator (NCBI) compare
b1158 pin +0.5 2.7 e14 prophage; site-specific DNA recombinase (NCBI) compare
b4195 ulaC +0.5 1.6 L-ascorbate-specific enzyme IIA component of PTS (NCBI) compare
b1629 rsxC +0.5 3.4 electron transport complex protein RnfC (NCBI) compare
b4698 mgrR +0.5 1.7 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b3753 rbsR +0.5 4.0 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b1533 ydeD +0.5 1.3 orf, hypothetical protein (VIMSS) compare
b4233 mpl +0.5 3.8 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (NCBI) compare
b0623 cspE +0.5 1.1 cold shock protein E (NCBI) compare
b3194 yrbE +0.5 3.6 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1594 dgsA +0.5 1.8 DNA-binding transcriptional repressor (NCBI) compare
b4139 aspA +0.5 3.4 aspartate ammonia-lyase (aspartase) (VIMSS) compare
b0955 ycbZ +0.5 3.4 predicted peptidase (NCBI) compare
b3062 ttdB +0.5 2.2 L(+)-tartrate dehydratase (NCBI) compare
b0468 ybaN +0.5 2.5 conserved inner membrane protein (NCBI) compare
b4646 yrdE +0.5 1.8 no description compare
b1462 b1462 +0.5 1.3 orf, hypothetical protein (VIMSS) compare
b3822 recQ +0.5 3.7 ATP-dependent DNA helicase (NCBI) compare
b1573 ydfC +0.5 0.8 Qin prophage; predicted protein (RefSeq) compare
b0216 aspV +0.5 2.4 tRNA-Asp (NCBI) compare
b1362 rzpR +0.5 1.2 putative Rac prophage endopeptidase (VIMSS) compare
b0585 fes +0.5 2.8 enterobactin/ferric enterobactin esterase (NCBI) compare
b2092 gatC +0.5 2.3 galactitol-specific enzyme IIC component of PTS (NCBI) compare
b0643 ybeL +0.5 2.3 hypothetical protein (NCBI) compare
b0989 cspH +0.5 0.9 stress protein, member of the CspA-family (NCBI) compare
b0583 entD +0.5 2.3 phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex (NCBI) compare
b0440 hupB +0.4 2.0 HU, DNA-binding transcriptional regulator, beta subunit (NCBI) compare
b4381 deoC +0.4 2.3 deoxyribose-phosphate aldolase (NCBI) compare
b0882 clpA +0.4 3.2 ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI) compare
b0191 yaeJ +0.4 1.6 hypothetical protein (NCBI) compare
b1651 gloA +0.4 1.6 glyoxalase I, Ni-dependent (NCBI) compare
b3195 yrbF +0.4 3.4 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b1151 ymfO +0.4 1.3 e14 prophage; conserved protein (RefSeq) compare
b1147 ymfL +0.4 1.1 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b1020 phoH +0.4 2.6 conserved protein with nucleoside triphosphate hydrolase domain (NCBI) compare
b2659 b2659 +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b1171 b1171 +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b2953 yggU +0.4 0.8 orf, hypothetical protein (VIMSS) compare
b3133 agaV +0.4 1.5 PTS system, cytoplasmic, N-acetylgalactosamine-specific IIB component 2 (EIIB-AGA) (VIMSS) compare
b4648 ysaC +0.4 1.5 no description compare
b0976 hyaE +0.4 1.8 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b2085 b2085 +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b1675 ydhZ +0.4 1.9 hypothetical protein (NCBI) compare
b0249 ykfF +0.4 1.3 CP4-6 prophage; predicted protein (NCBI) compare
b3775 ppiC +0.4 1.9 peptidyl-prolyl cis-trans isomerase C (rotamase C) (NCBI) compare
b4520 ymgF +0.4 0.7 hypothetical protein (NCBI) compare
b2918 argK +0.4 1.9 arginine/ornithine transport system ATPase (NCBI) compare
b1754 b1754 +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b3781 trxA +0.4 1.3 thioredoxin 1 (VIMSS) compare
b1486 ddpB +0.4 0.8 D-ala-D-ala transporter subunit (NCBI) compare
b4394 yjjX +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b3289 rsmB +0.4 3.2 16S rRNA m5C967 methyltransferase, S-adenosyl-L-methionine-dependent (NCBI) compare
b1731 cedA +0.4 1.3 cell division modulator (RefSeq) compare
b2346 vacJ +0.4 2.4 predicted lipoprotein (NCBI) compare
b3205 yhbJ +0.4 1.8 hypothetical protein (NCBI) compare
b2366 dsdA +0.4 1.7 D-serine dehydratase (NCBI) compare
b3448 yhhA +0.4 1.4 hypothetical protein (NCBI) compare
b1157 stfE +0.4 2.1 no description compare
b4203 rplI +0.4 1.8 50S ribosomal protein L9 (NCBI) compare
b3582 sgbU +0.4 1.6 probable 3-hexulose-6-phosphate isomerase (VIMSS) compare
b3497 yhiQ +0.4 2.6 predicted SAM-dependent methyltransferase (NCBI) compare
b4383 deoB +0.4 2.6 phosphopentomutase (NCBI) compare
b2189 proL +0.4 0.9 tRNA-Pro (NCBI) compare
b0435 bolA +0.4 2.0 possible regulator of murein genes (VIMSS) compare
b0346 mhpR +0.4 1.6 DNA-binding transcriptional activator, 3HPP-binding (RefSeq) compare


Specific Phenotypes

For 4 genes in this experiment

For stress Trimethoprim in Escherichia coli BW25113

For stress Trimethoprim across organisms