Experiment set12IT028 for Escherichia coli BW25113
LB with Trimethoprim 0.0025 mM
Group: stressMedia: LB + Trimethoprim (0.0025 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Keio_ML9a, 24-well transparent microplate; Multitron, Aerobic, at 37 (C), shaken=700 rpm
Growth: about 5.4 generations
By: Adam on 29-Jun-16
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: TnSeq_Plate1 C3
Specific Phenotypes
For 4 genes in this experiment
For stress Trimethoprim in Escherichia coli BW25113
For stress Trimethoprim across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| NAD and NADP cofactor biosynthesis global | 1 |
| NAD regulation | 1 |
| Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate | 1 |
| Redox-dependent regulation of nucleus processes | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Nicotinate and nicotinamide metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| NAD salvage pathway V (PNC V cycle) | 5 | 3 | 1 |
| NAD salvage pathway I (PNC VI cycle) | 7 | 5 | 1 |
| NAD salvage (plants) | 11 | 5 | 1 |
| superpathway of NAD biosynthesis in eukaryotes | 14 | 7 | 1 |