Experiment set11S543 for Escherichia coli BL21

Tailocin 256

Group: stress
Media: LB + Tailocin 256
Culturing: BL21_ML4, at 37 (C)
By: Cameron on 3/10/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 34 genes in this experiment

For stress Tailocin 256 in Escherichia coli BL21

For stress Tailocin 256 across organisms

SEED Subsystems

Subsystem	#Specific
Capsular Polysaccharide (CPS) of Campylobacter	2
Peptidoglycan Biosynthesis	2
Transport of Iron	2
ATP-dependent RNA helicases, bacterial	1
Bacterial hemoglobins	1
D-ribose utilization	1
Flavohaemoglobin	1
Glutaredoxins	1
Glycine and Serine Utilization	1
Hfl operon	1
Iron acquisition in Vibrio	1
Mannitol Utilization	1
Polyadenylation bacterial	1
Pyruvate Alanine Serine Interconversions	1
Rhamnose containing glycans	1
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization	1
Threonine anaerobic catabolism gene cluster	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Peptidoglycan biosynthesis
• Lipopolysaccharide biosynthesis
• Fructose and mannose metabolism
• Purine metabolism
• Biotin metabolism
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Pentose phosphate pathway
• Pentose and glucuronate interconversions
• Ubiquinone and menaquinone biosynthesis
• Lysine degradation
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Keratan sulfate biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Naphthalene and anthracene degradation
• Nicotinate and nicotinamide metabolism
• Zeatin biosynthesis
• Phenylpropanoid biosynthesis
• Flavone and flavonol biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
mannitol degradation I	1	1	1
siroheme biosynthesis	4	4	2
tetrahydropteridine recycling	2	2	1
D-arabinose degradation I	2	2	1
polyphosphate metabolism	2	2	1
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
L-threonine degradation II	2	2	1
8-amino-7-oxononanoate biosynthesis III	2	1	1
8-amino-7-oxononanoate biosynthesis II	2	1	1
ribitol degradation I	2	1	1
D-arabinose degradation V	3	1	1
factor 430 biosynthesis	7	3	2
peptidoglycan maturation (meso-diaminopimelate containing)	12	6	3
CMP-3-deoxy-D-manno-octulosonate biosynthesis	5	5	1
polyisoprenoid biosynthesis (E. coli)	5	5	1
8-amino-7-oxononanoate biosynthesis IV	5	4	1
mannitol cycle	5	4	1
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	3
peptidoglycan biosynthesis II (staphylococci)	17	12	3
ppGpp metabolism	6	6	1
biotin biosynthesis II	6	4	1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)	13	2	2
CMP-8-amino-3,8-dideoxy-D-manno-octulosonate biosynthesis	7	5	1
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)	15	3	2
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
8-amino-7-oxononanoate biosynthesis I	11	11	1
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
biotin biosynthesis I	15	15	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1
adenosylcobalamin biosynthesis II (aerobic)	33	17	2
superpathway of (Kdo)2-lipid A biosynthesis	17	17	1
superpathway of hexitol degradation (bacteria)	18	18	1
superpathway of L-threonine metabolism	18	16	1
adenosylcobalamin biosynthesis I (anaerobic)	36	16	2
hexitol fermentation to lactate, formate, ethanol and acetate	19	19	1
superpathway of Kdo2-lipid A biosynthesis	25	21	1
superpathway of pentose and pentitol degradation	42	15	1