Experiment set11IT077 for Pseudomonas putida KT2440

carbon source Glycerol 108.6 mM

Group: carbon source
Media: MOPS minimal media_noCarbon + Glycerol (108.6 mM)
Culturing: Putida_ML5_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
By: Thomas Eng on 09/16/2018
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 5 genes in this experiment

For carbon source Glycerol in Pseudomonas putida KT2440

For carbon source Glycerol across organisms

SEED Subsystems

Subsystem	#Specific
ATP-dependent RNA helicases, bacterial	1
High affinity phosphate transporter and control of PHO regulon	1
Methionine Biosynthesis	1
Phosphate metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.