Experiment set11IT023 for Agrobacterium fabrum C58
methyl-alpha-D-xylopyranoside carbon source
Group: carbon sourceMedia: MOPS minimal media_noCarbon + methyl-alpha-D-xylopyranoside (5 mM), pH=7
Culturing: Agro_ML11, 24-well plate, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 1/6/22
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 7 genes in this experiment
For carbon source methyl-alpha-D-xylopyranoside in Agrobacterium fabrum C58
For carbon source methyl-alpha-D-xylopyranoside across organisms
SEED Subsystems
Subsystem | #Specific |
---|---|
Xylose utilization | 3 |
Inositol catabolism | 1 |
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pentose and glucuronate interconversions
- Fructose and mannose metabolism
- 1- and 2-Methylnaphthalene degradation
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
Pathway | #Steps | #Present | #Specific |
---|---|---|---|
D-xylose degradation I | 2 | 2 | 2 |
xylitol degradation I | 2 | 2 | 1 |
D-arabinitol degradation I | 2 | 1 | 1 |
superpathway of glucose and xylose degradation | 17 | 16 | 2 |
superpathway of pentose and pentitol degradation | 42 | 16 | 2 |