Experiment set11IT003 for Agrobacterium fabrum C58

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Ethyl benzoate carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Ethyl benzoate (2.5 mM), pH=7
Culturing: Agro_ML11, 24-well plate, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 1/6/22
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 15 genes in this experiment

For carbon source Ethyl benzoate in Agrobacterium fabrum C58

For carbon source Ethyl benzoate across organisms

SEED Subsystems

Subsystem #Specific
Multidrug Resistance Efflux Pumps 3
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 2
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon) 2
Bacterial RNA-metabolizing Zn-dependent hydrolases 1
Biotin biosynthesis 1
Entner-Doudoroff Pathway 1
Fermentations: Mixed acid 1
Glycolate, glyoxylate interconversions 1
Methylglyoxal Metabolism 1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 1
n-Phenylalkanoic acid degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
long-chain fatty acid activation 1 1 1
acetaldehyde biosynthesis I 1 1 1
phytol degradation 4 3 3
ethanol degradation II 3 3 2
putrescine degradation V 2 2 1
3-methyl-branched fatty acid α-oxidation 6 3 3
putrescine degradation I 2 1 1
γ-linolenate biosynthesis II (animals) 2 1 1
ethanol degradation I 2 1 1
linoleate biosynthesis II (animals) 2 1 1
ethylene glycol degradation 2 1 1
pyruvate fermentation to ethanol II 2 1 1
sphingosine and sphingosine-1-phosphate metabolism 10 4 4
octane oxidation 5 2 2
ethanol degradation IV 3 3 1
putrescine degradation IV 3 2 1
L-valine degradation II 3 2 1
L-isoleucine degradation II 3 2 1
L-leucine degradation III 3 2 1
hypotaurine degradation 3 2 1
ethanol degradation III 3 2 1
histamine degradation 3 1 1
starch degradation I 3 1 1
L-methionine degradation III 3 1 1
pyruvate fermentation to ethanol III 3 1 1
oleate biosynthesis I (plants) 3 1 1
alkane biosynthesis II 3 1 1
pyruvate fermentation to ethanol I 3 1 1
noradrenaline and adrenaline degradation 13 4 4
serotonin degradation 7 3 2
ceramide degradation by α-oxidation 7 2 2
L-tryptophan degradation X (mammalian, via tryptamine) 4 2 1
L-phenylalanine degradation III 4 2 1
fatty acid α-oxidation I (plants) 4 2 1
L-tyrosine degradation III 4 2 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 4
D-arabinose degradation II 4 1 1
salidroside biosynthesis 4 1 1
wax esters biosynthesis II 4 1 1
putrescine degradation III 4 1 1
cytidine-5'-diphosphate-glycerol biosynthesis 4 1 1
phosphatidylcholine acyl editing 4 1 1
long chain fatty acid ester synthesis (engineered) 4 1 1
sporopollenin precursors biosynthesis 18 4 4
pyruvate fermentation to isobutanol (engineered) 5 4 1
mitochondrial NADPH production (yeast) 5 3 1
(S)-propane-1,2-diol degradation 5 1 1
acetylene degradation (anaerobic) 5 1 1
phenylethanol biosynthesis 5 1 1
dopamine degradation 5 1 1
ethanolamine utilization 5 1 1
fatty acid salvage 6 5 1
stearate biosynthesis II (bacteria and plants) 6 5 1
stearate biosynthesis IV 6 4 1
6-gingerol analog biosynthesis (engineered) 6 2 1
stearate biosynthesis I (animals) 6 1 1
alkane oxidation 6 1 1
3-methylbutanol biosynthesis (engineered) 7 6 1
superpathway of glycol metabolism and degradation 7 4 1
arachidonate biosynthesis III (6-desaturase, mammals) 7 1 1
icosapentaenoate biosynthesis II (6-desaturase, mammals) 7 1 1
icosapentaenoate biosynthesis III (8-desaturase, mammals) 7 1 1
capsaicin biosynthesis 7 1 1
limonene degradation IV (anaerobic) 7 1 1
glycogen degradation I 8 6 1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) 8 6 1
superpathway of ornithine degradation 8 4 1
butanol and isobutanol biosynthesis (engineered) 8 3 1
2-deoxy-D-ribose degradation II 8 2 1
aromatic biogenic amine degradation (bacteria) 8 1 1
Entner-Doudoroff pathway II (non-phosphorylative) 9 5 1
superpathway of fermentation (Chlamydomonas reinhardtii) 9 3 1
suberin monomers biosynthesis 20 3 2
superpathway of fatty acid biosynthesis II (plant) 43 38 4
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 6 1
superpathway of L-arginine and L-ornithine degradation 13 8 1
superpathway of Clostridium acetobutylicum solventogenic fermentation 13 5 1
L-tryptophan degradation V (side chain pathway) 13 1 1
palmitate biosynthesis II (type II fatty acid synthase) 31 29 2
mixed acid fermentation 16 9 1
cutin biosynthesis 16 1 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 5 1
heterolactic fermentation 18 12 1
superpathway of anaerobic sucrose degradation 19 13 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 10 1
superpathway of N-acetylneuraminate degradation 22 12 1
superpathway of fatty acids biosynthesis (E. coli) 53 49 2
palmitate biosynthesis III 29 28 1
oleate β-oxidation 35 27 1
superpathway of pentose and pentitol degradation 42 16 1