Experiment set10S151 for Escherichia coli BL21

D-Glucose

Group: carbon source
Media: M9_plus_D-Glucose + D-Glucose (20 mM)
Culturing: BL21_ML4, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 12/16/24

Specific Phenotypes

For 5 genes in this experiment

For carbon source D-Glucose in Escherichia coli BL21

For carbon source D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
Cysteine Biosynthesis	4
Ammonia assimilation	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glutamate metabolism
• Nitrogen metabolism
• Sulfur metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Purine metabolism
• Selenoamino acid metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamate biosynthesis I	2	2	2
L-glutamine degradation I	1	1	1
L-glutamate biosynthesis III	1	1	1
L-glutamate biosynthesis IV	1	1	1
L-glutamine degradation II	1	1	1
ammonia assimilation cycle III	3	3	2
assimilatory sulfate reduction III	3	3	2
L-glutamate and L-glutamine biosynthesis	7	5	4
assimilatory sulfate reduction I	4	4	2
ammonia assimilation cycle I	2	2	1
sulfate activation for sulfonation	2	2	1
superpathway of ammonia assimilation (plants)	3	2	1
assimilatory sulfate reduction II	3	1	1
sulfite oxidation III	3	1	1
assimilatory sulfate reduction IV	4	3	1
L-asparagine biosynthesis III (tRNA-dependent)	4	1	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	1	1
superpathway of sulfate assimilation and cysteine biosynthesis	9	9	2
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)	10	6	2
selenate reduction	5	2	1
dissimilatory sulfate reduction I (to hydrogen sufide))	5	1	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	10	2
superpathway of sulfur metabolism (Desulfocapsa sulfoexigens)	6	1	1
superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans)	6	1	1
L-citrulline biosynthesis	8	6	1
superpathway of L-citrulline metabolism	12	8	1
superpathway of sulfide oxidation (phototrophic sulfur bacteria)	12	2	1
superpathway of seleno-compound metabolism	19	6	1