Experiment set10IT095 for Escherichia coli BW25113

Compare to:

LB Anaerobic with Norfloxacin 0.00000015625 mM

200 most detrimental genes:

  gene name fitness t score description  
b3405 ompR +7.9 26.3 osmolarity response regulator (NCBI) compare
b0929 ompF +6.9 30.5 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b0464 acrR +4.2 29.3 DNA-binding transcriptional repressor (NCBI) compare
b1530 marR +3.3 5.5 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b0178 hlpA +2.7 5.5 periplasmic chaperone (NCBI) compare
b2572 rseA +2.7 7.0 anti-sigma factor (NCBI) compare
b1185 dsbB +2.4 5.2 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b3860 dsbA +2.2 14.7 periplasmic protein disulfide isomerase I (NCBI) compare
b2617 smpA +1.7 4.0 small membrane protein A (VIMSS) compare
b1151 ymfO +1.7 4.2 e14 prophage; conserved protein (RefSeq) compare
b2512 yfgL +1.7 8.1 protein assembly complex, lipoprotein component (NCBI) compare
b1865 nudB +1.6 2.6 dATP pyrophosphohydrolase (NCBI) compare
b3162 deaD +1.6 6.9 inducible ATP-independent RNA helicase (VIMSS) compare
b1236 galU +1.5 6.2 glucose-1-phosphate uridylyltransferase (NCBI) compare
b4699 fnrS +1.5 1.9 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b3673 emrD +1.5 10.9 2-module integral membrane pump; multidrug resistance (VIMSS) compare
b3751 rbsB +1.5 4.7 D-ribose transporter subunit (NCBI) compare
b4692 oweE +1.5 2.4 no description compare
b2571 rseB +1.4 7.6 periplasmic negative regulator of sigmaE (NCBI) compare
b4041 plsB +1.4 4.2 glycerol-3-phosphate acyltransferase (VIMSS) compare
b1048 mdoG +1.4 7.9 glucan biosynthesis protein, periplasmic (NCBI) compare
b3181 greA +1.4 5.8 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1147 ymfL +1.4 3.2 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b0437 clpP +1.4 5.8 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b1150 ymfR +1.4 2.0 e14 prophage; predicted protein (NCBI) compare
b1049 mdoH +1.4 9.2 glucosyltransferase MdoH (NCBI) compare
b1349 recT +1.4 5.1 Rac prophage; recombination and repair protein (NCBI) compare
b1187 fadR +1.4 3.0 fatty acid metabolism regulator (NCBI) compare
b0059 hepA +1.4 11.2 ATP-dependent helicase HepA (NCBI) compare
b0232 yafN +1.3 1.4 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b1855 lpxM +1.3 5.8 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b4693 aaaE +1.3 5.8 no description compare
b0889 lrp +1.3 3.5 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b0928 aspC +1.3 3.1 aspartate aminotransferase, PLP-dependent (NCBI) compare
b1850 eda +1.3 3.7 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b3706 trmE +1.3 4.6 tRNA modification GTPase (NCBI) compare
b3741 gidA +1.2 5.8 glucose-inhibited division protein A (NCBI) compare
b1613 manA +1.2 2.7 mannose-6-phosphate isomerase (VIMSS) compare
b1157 stfE +1.2 7.5 no description compare
b3026 qseC +1.2 4.0 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b1921 fliZ +1.2 3.4 orf, hypothetical protein (VIMSS) compare
b1153 ymfQ +1.1 3.7 e14 prophage; conserved protein (NCBI) compare
b1154 ycfK +1.1 5.7 e14 prophage; predicted protein (NCBI) compare
b2912 ygfA +1.1 3.4 putative ligase (VIMSS) compare
b4397 creA +1.1 1.4 hypothetical protein (NCBI) compare
b1111 ycfQ +1.1 3.1 orf, hypothetical protein (VIMSS) compare
b1152 ymfP +1.1 4.7 orf, hypothetical protein (VIMSS) compare
b4685 yrbN +1.1 2.6 expressed protein (RefSeq) compare
b0065 yabI +1.1 5.4 conserved inner membrane protein (NCBI) compare
b3204 ptsN +1.0 2.7 sugar-specific enzyme IIA component of PTS (NCBI) compare
b1138 ymfE +1.0 2.1 e14 prophage; predicted inner membrane protein (NCBI) compare
b3658 selC +1.0 3.6 tRNA-Sec (NCBI) compare
b1291 sapD +1.0 2.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2051 wcaH +1.0 2.2 GDP-mannose mannosyl hydrolase (VIMSS) compare
b2061 wzb +1.0 2.5 protein-tyrosine phosphatase (NCBI) compare
b4545 ypdJ +1.0 1.6 no description compare
b2419 yfeK +1.0 2.7 hypothetical protein (NCBI) compare
b1158 pin +1.0 5.5 e14 prophage; site-specific DNA recombinase (NCBI) compare
b4158 yjeO +1.0 2.6 conserved inner membrane protein (NCBI) compare
b1811 yoaH +1.0 3.5 hypothetical protein (NCBI) compare
b1630 rsxD +1.0 4.6 electron transport complex protein RnfD (NCBI) compare
b1144 ymfJ +1.0 1.8 orf, hypothetical protein (VIMSS) compare
b2951 yggS +1.0 4.2 predicted enzyme (NCBI) compare
b3791 rffA +0.9 5.2 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b1155 ymfS +0.9 4.2 e14 prophage; predicted protein (NCBI) compare
b3749 rbsA +0.9 5.7 fused D-ribose transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b0438 clpX +0.9 3.7 ATP-dependent protease ATP-binding subunit (NCBI) compare
b2289 lrhA +0.9 5.8 DNA-binding transcriptional repressor of flagellar, motility and chemotaxis genes (NCBI) compare
b2939 yqgB +0.9 2.0 predicted protein (RefSeq) compare
b2175 spr +0.9 3.9 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b1594 dgsA +0.9 3.1 DNA-binding transcriptional repressor (NCBI) compare
b4011 yjaA +0.9 3.5 hypothetical protein (NCBI) compare
b2501 ppk +0.9 6.1 polyphosphate kinase (NCBI) compare
b2570 rseC +0.9 4.1 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b4570 lomR +0.9 2.2 no description compare
b1627 rsxA +0.9 3.3 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b0191 yaeJ +0.9 4.1 hypothetical protein (NCBI) compare
b3229 sspA +0.9 2.4 stringent starvation protein A (NCBI) compare
b2904 gcvH +0.9 2.8 glycine cleavage system protein H (NCBI) compare
b1842 holE +0.9 1.3 DNA polymerase III, theta subunit (NCBI) compare
b1284 yciT +0.9 3.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b1582 ynfA +0.9 1.5 hypothetical protein (NCBI) compare
b3630 rfaP +0.8 6.4 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b3194 yrbE +0.8 5.4 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b3649 rpoZ +0.8 2.2 DNA-directed RNA polymerase subunit omega (NCBI) compare
b1438 ydcQ +0.8 3.2 predicted DNA-binding transcriptional regulator (RefSeq) compare
b3790 rffC +0.8 3.2 TDP-fucosamine acetyltransferase (RefSeq) compare
b0418 pgpA +0.8 4.0 phosphatidylglycerophosphatase A (NCBI) compare
b1949 fliQ +0.8 1.5 flagellar biosynthesis protein Q (NCBI) compare
b3205 yhbJ +0.8 3.0 hypothetical protein (NCBI) compare
b2058 wcaB +0.8 1.1 predicted acyl transferase (NCBI) compare
b2947 gshB +0.8 2.2 glutathione synthetase (NCBI) compare
b3750 rbsC +0.8 3.9 ribose ABC transporter permease protein (NCBI) compare
b3842 rfaH +0.8 4.3 transcriptional activator RfaH (NCBI) compare
b1480 sra +0.8 1.0 30S ribosomal subunit protein S22 (NCBI) compare
b1524 yneH +0.8 0.9 predicted glutaminase (NCBI) compare
b3631 rfaG +0.8 6.4 glucosyltransferase I (NCBI) compare
b1618 uidR +0.8 2.2 DNA-binding transcriptional repressor (NCBI) compare
b4241 treR +0.8 6.1 trehalose repressor (NCBI) compare
b4063 soxR +0.8 2.3 DNA-binding transcriptional dual regulator, Fe-S center for redox-sensing (NCBI) compare
b1632 rsxE +0.8 4.9 NADH-ubiquinone oxidoreductase (NCBI) compare
b4651 yibW +0.7 0.4 no description compare
b0739 tolA +0.7 2.3 cell envelope integrity inner membrane protein TolA (NCBI) compare
b4363 yjjB +0.7 2.7 conserved inner membrane protein (RefSeq) compare
b3632 rfaQ +0.7 6.4 lipopolysaccharide core biosynthesis protein (NCBI) compare
b3620 rfaF +0.7 3.1 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b2749 ygbE +0.7 3.6 conserved inner membrane protein (NCBI) compare
b2214 yojL +0.7 3.4 predicted thiamine biosynthesis lipoprotein (NCBI) compare
b1778 yeaA +0.7 2.0 methionine sulfoxide reductase B (NCBI) compare
b1573 ydfC +0.7 1.1 Qin prophage; predicted protein (RefSeq) compare
b2096 gatY +0.7 3.4 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b1350 recE +0.7 3.7 Rac prophage; exonuclease VIII, 5' -> 3' specific dsDNA exonuclease (NCBI) compare
b4508 peaD +0.7 2.6 putative sensory transduction regulator (VIMSS) compare
b2024 hisA +0.7 2.1 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b4451 ryhB +0.7 1.1 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b2259 pmrD +0.7 0.8 polymyxin resistance protein B (VIMSS) compare
b1992 cobS +0.7 1.3 cobalamin synthase (NCBI) compare
b3193 yrbD +0.7 4.2 predicted ABC-type organic solvent transporter (NCBI) compare
b2569 lepA +0.6 2.1 GTP-binding protein LepA (NCBI) compare
b2792 yqcC +0.6 1.3 hypothetical protein (NCBI) compare
b1766 sppA +0.6 4.0 protease IV (signal peptide peptidase) (NCBI) compare
b1434 ydcN +0.6 1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b4589 ylcI +0.6 1.5 hypothetical protein (RefSeq) compare
b1608 rstA +0.6 2.6 DNA-binding response regulator in two-component regulatory system with RstB (RefSeq) compare
b4481 rffT +0.6 3.3 4-alpha-L-fucosyltransferase (NCBI) compare
b1593 b1593 +0.6 2.1 orf, hypothetical protein (VIMSS) compare
b1139 lit +0.6 2.6 e14 prophage; cell death peptidase, inhibitor of T4 late gene expression (NCBI) compare
b1160 elbA +0.6 0.6 hypothetical protein (NCBI) compare
b3916 pfkA +0.6 1.7 6-phosphofructokinase (NCBI) compare
b2708 gutQ +0.6 2.8 orf, hypothetical protein (VIMSS) compare
b1134 ymfB +0.6 1.0 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b1557 cspB +0.6 1.3 Qin prophage; cold shock protein (NCBI) compare
b1960 vsr +0.6 2.2 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b0798 ybiA +0.6 0.9 hypothetical protein (NCBI) compare
b3190 yrbA +0.6 2.2 orf, hypothetical protein (VIMSS) compare
b3977 tyrU +0.6 0.6 tRNA-Tyr (NCBI) compare
b1867 yecD +0.6 2.2 orf, hypothetical protein (VIMSS) compare
b3146 yraL +0.6 3.1 predicted methyltransferase (NCBI) compare
b0904 focA +0.6 2.0 formate transporter (NCBI) compare
b1739 osmE +0.6 1.5 DNA-binding transcriptional activator (NCBI) compare
b0122 yacC +0.6 1.8 orf, hypothetical protein (VIMSS) compare
b3725 pstB +0.6 0.6 phosphate transporter subunit (NCBI) compare
b0220 ivy +0.6 2.2 inhibitor of vertebrate C-lysozyme (NCBI) compare
b2142 yohK +0.6 3.1 predicted inner membrane protein (NCBI) compare
b4416 rybA +0.6 1.0 unknown RNA (NCBI) compare
b3484 yhhI +0.6 1.3 predicted transposase (NCBI) compare
b4460 araH +0.6 1.3 fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b0759 galE +0.6 2.6 UDP-galactose-4-epimerase (VIMSS) compare
b1266 yciV +0.6 1.8 hypothetical protein (NCBI) compare
b1831 proQ +0.6 1.8 putative solute/DNA competence effector (NCBI) compare
b2092 gatC +0.6 2.4 galactitol-specific enzyme IIC component of PTS (NCBI) compare
b3619 rfaD +0.5 1.9 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b2083 yegZ +0.5 0.9 orf, hypothetical protein (VIMSS) compare
b0010 yaaH +0.5 1.9 conserved inner membrane protein associated with acetate transport (NCBI) compare
b2093 gatB +0.5 1.7 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b1684 sufA +0.5 1.0 iron-sulfur cluster assembly scaffold protein (NCBI) compare
b1325 ycjG +0.5 2.2 putative muconate cycloisomerase I (EC 5.5.-.-) (VIMSS) compare
b2195 ccmG +0.5 1.7 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b0247 ykfG +0.5 0.8 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b4596 yciZ +0.5 0.9 hypothetical protein (RefSeq) compare
b1281 pyrF +0.5 1.4 orotidine 5'-phosphate decarboxylase (NCBI) compare
b2044 wcaL +0.5 2.4 predicted glycosyl transferase (NCBI) compare
b1034 ycdX +0.5 2.4 hypothetical protein (NCBI) compare
b2229 b2229 +0.5 1.9 orf, hypothetical protein (VIMSS) compare
b0249 ykfF +0.5 1.0 CP4-6 prophage; predicted protein (NCBI) compare
b4679 yohP +0.5 1.5 expressed protein (RefSeq) compare
b2741 rpoS +0.5 2.4 RNA polymerase sigma factor (NCBI) compare
b0979 appB +0.5 1.9 cytochrome bd-II oxidase, subunit II (NCBI) compare
b1305 pspB +0.5 1.5 phage shock protein B (NCBI) compare
b2317 dedA +0.5 1.6 conserved inner membrane protein (NCBI) compare
b1469 narU +0.5 2.1 nitrate/nitrite transporter (NCBI) compare
b0862 artQ +0.5 1.7 arginine transporter subunit (NCBI) compare
b4613 dinQ +0.5 1.2 Damage inducible, function unknown (RefSeq) compare
b4438 cyaR +0.5 1.0 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b1511 ydeV +0.5 1.9 putative kinase (VIMSS) compare
b1954 dsrA +0.5 0.7 regulatory antisense RNA (NCBI) compare
b2209 eco +0.5 1.0 ecotin precursor (NCBI) compare
b1507 hipA +0.5 2.1 regulator with hipB (NCBI) compare
b0849 grxA +0.5 0.8 glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) (NCBI) compare
b2063 yegH +0.5 2.5 putative transport protein (VIMSS) compare
b1558 cspF +0.5 1.1 Qin prophage; cold shock protein (NCBI) compare
b1336 ydaH +0.5 2.0 putative pump protein (transport) (VIMSS) compare
b0632 dacA +0.5 2.6 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b1467 narY +0.5 2.4 nitrate reductase 2 (NRZ), beta subunit (NCBI) compare
b4453 ldrD +0.5 0.9 toxic polypeptide, small (NCBI) compare
b1707 ydiV +0.5 1.6 hypothetical protein (NCBI) compare
b0338 cynR +0.5 1.7 DNA-binding transcriptional dual regulator (NCBI) compare
b0450 glnK +0.5 0.9 nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB (NCBI) compare
b1673 ydhV +0.5 2.1 predicted oxidoreductase (NCBI) compare
b0219 yafV +0.5 1.1 predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI) compare
b2237 inaA +0.5 1.2 hypothetical protein (NCBI) compare
b4627 ykfL +0.5 1.4 no description compare
b1640 ydhH +0.5 1.8 anhydro-N-acetylmuramic acid kinase (NCBI) compare
b0523 purE +0.5 1.8 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b3057 bacA +0.5 2.2 undecaprenyl pyrophosphate phosphatase (NCBI) compare
b1520 b1520 +0.5 1.6 orf, hypothetical protein (VIMSS) compare
b2560 yfhB +0.4 2.2 orf, hypothetical protein (VIMSS) compare
b1412 azoR +0.4 1.4 acyl carrier protein phosphodiesterase (NCBI) compare
b1312 ycjP +0.4 1.6 predicted sugar transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1629 rsxC +0.4 2.3 electron transport complex protein RnfC (NCBI) compare


Specific Phenotypes

For 8 genes in this experiment

For stress Norfloxacin in Escherichia coli BW25113

For stress Norfloxacin across organisms