Experiment set10IT084 for Escherichia coli BW25113

Compare to:

LB Anaerobic with Nalidixic acid sodium salt 0.00000195313 mM

200 most detrimental genes:

  gene name fitness t score description  
b2289 lrhA +2.2 14.6 DNA-binding transcriptional repressor of flagellar, motility and chemotaxis genes (NCBI) compare
b3341 rpsG +1.5 2.5 30S ribosomal protein S7 (NCBI) compare
b3764 yifE +1.4 4.9 hypothetical protein (NCBI) compare
b1231 tyrT +1.3 1.6 tRNA-Tyr (NCBI) compare
b1217 chaB +1.3 1.9 cation transport regulator (NCBI) compare
b4480 hdfR +1.2 7.0 transcriptional regulator HdfR (NCBI) compare
b3979 thrT +1.1 0.6 tRNA-Thr (NCBI) compare
b4596 yciZ +1.0 2.5 hypothetical protein (RefSeq) compare
b2683 ygaH +1.0 5.8 predicted inner membrane protein (NCBI) compare
b1811 yoaH +1.0 3.7 hypothetical protein (NCBI) compare
b2419 yfeK +0.9 1.8 hypothetical protein (NCBI) compare
b4589 ylcI +0.9 3.0 hypothetical protein (RefSeq) compare
b1293 sapB +0.9 1.6 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1642 slyA +0.9 2.0 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b3753 rbsR +0.9 6.4 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b1593 b1593 +0.9 2.4 orf, hypothetical protein (VIMSS) compare
b1613 manA +0.9 1.6 mannose-6-phosphate isomerase (VIMSS) compare
b4041 plsB +0.9 2.3 glycerol-3-phosphate acyltransferase (VIMSS) compare
b1292 sapC +0.9 1.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1842 holE +0.8 1.5 DNA polymerase III, theta subunit (NCBI) compare
b2659 b2659 +0.8 1.9 orf, hypothetical protein (VIMSS) compare
b1380 ldhA +0.8 2.3 D-lactate dehydrogenase (NCBI) compare
b2093 gatB +0.8 3.5 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b3029 ygiN +0.8 2.0 quinol monooxygenase (NCBI) compare
b2684 mprA +0.8 3.3 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b4533 ynfO +0.8 1.8 Qin prophage; predicted protein (NCBI) compare
b2096 gatY +0.8 2.5 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b2518 ndk +0.8 2.0 nucleoside diphosphate kinase (NCBI) compare
b2217 rcsB +0.7 4.1 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b0678 nagB +0.7 2.9 glucosamine-6-phosphate deaminase (NCBI) compare
b2047 wcaJ +0.7 2.7 predicted UDP-glucose lipid carrier transferase (NCBI) compare
b4578 insP +0.7 2.0 no description compare
b0889 lrp +0.7 1.5 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b1827 kdgR +0.7 2.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1263 trpD +0.7 1.7 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b0858 ybjO +0.7 1.4 predicted inner membrane protein (NCBI) compare
b4450 arcZ +0.7 1.8 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b2614 grpE +0.7 1.3 heat shock protein (NCBI) compare
b1548 nohA +0.7 1.4 Qin prophage; predicted packaging protein (RefSeq) compare
b1446 ydcY +0.6 1.6 hypothetical protein (NCBI) compare
b2156 lysP +0.6 4.3 lysine transporter (NCBI) compare
b2259 pmrD +0.6 0.7 polymyxin resistance protein B (VIMSS) compare
b4195 ulaC +0.6 2.1 L-ascorbate-specific enzyme IIA component of PTS (NCBI) compare
b0906 ycaP +0.6 3.0 conserved inner membrane protein (NCBI) compare
b1627 rsxA +0.6 2.3 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b1516 lsrB +0.6 2.6 AI2 transporter (NCBI) compare
b4675 yoaJ +0.6 2.2 expressed protein (RefSeq) compare
b2679 proX +0.6 2.7 glycine betaine transporter subunit (NCBI) compare
b0367 tauC +0.6 2.8 taurine transporter subunit (NCBI) compare
b0935 ssuD +0.6 2.8 alkanesulfonate monooxygenase (NCBI) compare
b2634 yfjR +0.6 2.6 CP4-57 prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1312 ycjP +0.6 2.7 predicted sugar transporter subunit: membrane component of ABC superfamily (NCBI) compare
b3752 rbsK +0.6 4.1 ribokinase (NCBI) compare
b1622 malY +0.6 1.7 bifunctional beta-cystathionase, PLP-dependent/ regulator of maltose regulon (NCBI) compare
b4484 cpxP +0.6 2.3 periplasmic protein combats stress (NCBI) compare
b1846 yebE +0.6 2.1 hypothetical protein (NCBI) compare
b0776 bioF +0.6 3.2 8-amino-7-oxononanoate synthase (NCBI) compare
b4416 rybA +0.6 1.0 unknown RNA (NCBI) compare
b4592 yccB +0.6 1.3 hypothetical protein (RefSeq) compare
b4613 dinQ +0.6 1.3 Damage inducible, function unknown (RefSeq) compare
b2939 yqgB +0.6 0.9 predicted protein (RefSeq) compare
b1582 ynfA +0.6 1.3 hypothetical protein (NCBI) compare
b0866 ybjQ +0.6 1.0 hypothetical protein (NCBI) compare
b1520 b1520 +0.6 2.0 orf, hypothetical protein (VIMSS) compare
b4692 oweE +0.6 1.7 no description compare
b1433 b1433 +0.6 2.6 putative membrane transport protein (VIMSS) compare
b1134 ymfB +0.6 1.2 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b4436 ryeC +0.5 1.0 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b0556 rzpD +0.5 1.4 DLP12 prophage; predicted murein endopeptidase (NCBI) compare
b2015 yeeY +0.5 3.0 putative transcriptional regulator LYSR-type (VIMSS) compare
b1427 rimL +0.5 1.6 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b2009 sbmC +0.5 2.2 DNA gyrase inhibitor (NCBI) compare
b1349 recT +0.5 2.5 Rac prophage; recombination and repair protein (NCBI) compare
b1465 narV +0.5 1.5 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b0782 moaB +0.5 0.8 molybdopterin biosynthesis protein B (NCBI) compare
b1418 cybB +0.5 1.8 cytochrome b(561) (VIMSS) compare
b1006 ycdG +0.5 2.2 putative transport protein (VIMSS) compare
b4529 ydbJ +0.5 0.7 hypothetical protein (NCBI) compare
b2279 nuoK +0.5 0.8 NADH dehydrogenase subunit K (NCBI) compare
b1104 ycfL +0.5 0.8 hypothetical protein (NCBI) compare
b0977 hyaF +0.5 2.2 protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI) compare
b1821 b1821 +0.5 1.8 orf, hypothetical protein (VIMSS) compare
b4241 treR +0.5 3.7 trehalose repressor (NCBI) compare
b2298 yfcC +0.5 2.6 putative S-transferase (VIMSS) compare
b1706 ydiU +0.5 2.6 hypothetical protein (NCBI) compare
b4515 ybgT +0.5 1.0 hypothetical protein (NCBI) compare
b2732 ygbA +0.5 2.9 hypothetical protein (NCBI) compare
b1867 yecD +0.5 2.3 orf, hypothetical protein (VIMSS) compare
b1618 uidR +0.5 1.5 DNA-binding transcriptional repressor (NCBI) compare
b2072 yegK +0.5 2.7 hypothetical protein (NCBI) compare
b1362 rzpR +0.5 1.1 putative Rac prophage endopeptidase (VIMSS) compare
b2682 ygaZ +0.5 1.5 predicted transporter (NCBI) compare
b1684 sufA +0.5 0.9 iron-sulfur cluster assembly scaffold protein (NCBI) compare
b2706 gutM +0.5 1.0 DNA-binding transcriptional activator of glucitol operon (NCBI) compare
b0418 pgpA +0.5 2.5 phosphatidylglycerophosphatase A (NCBI) compare
b2058 wcaB +0.5 0.6 predicted acyl transferase (NCBI) compare
b2629 yfjM +0.5 2.9 CP4-57 prophage; predicted protein (NCBI) compare
b0250 ykfB +0.5 2.0 CP4-6 prophage; predicted protein (NCBI) compare
b1109 ndh +0.5 1.8 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b0503 ybbB +0.5 2.0 tRNA 2-selenouridine synthase, selenophosphate-dependent (NCBI) compare
b1853 yebK +0.5 1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b4545 ypdJ +0.5 0.8 no description compare
b0961 yccF +0.5 1.7 conserved inner membrane protein (NCBI) compare
b2480 bcp +0.5 1.9 thioredoxin-dependent thiol peroxidase (NCBI) compare
b0991 ymcE +0.5 1.9 cold shock gene (NCBI) compare
b2605 yfiB +0.5 2.0 predicted outer membrane lipoprotein (NCBI) compare
b2290 yfbQ +0.5 3.4 aspartate aminotransferase (NCBI) compare
b2689 yqaA +0.5 1.3 conserved inner membrane protein (NCBI) compare
b4521 ycgI +0.5 1.6 hypothetical protein (NCBI) compare
b4551 yheV +0.5 1.5 hypothetical protein (NCBI) compare
b0127 yadG +0.5 1.8 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b0289 yagV +0.5 1.6 orf, hypothetical protein (VIMSS) compare
b0385 yaiC +0.4 2.3 predicted diguanylate cyclase (NCBI) compare
b3071 yqjI +0.4 1.7 predicted transcriptional regulator (NCBI) compare
b1473 yddG +0.4 2.4 predicted methyl viologen efflux pump (RefSeq) compare
b0831 yliC +0.4 2.2 predicted peptide transporter subunit: membrane component of ABC superfamily (NCBI) compare
b4282 yjhE +0.4 2.3 orf, hypothetical protein (VIMSS) compare
b1007 b1007 +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b1475 fdnH +0.4 2.2 formate dehydrogenase-N, Fe-S (beta) subunit, nitrate-inducible (NCBI) compare
b1988 nac +0.4 1.5 DNA-binding transcriptional dual regulator of nitrogen assimilation (NCBI) compare
b1135 ymfC +0.4 2.3 orf, hypothetical protein (VIMSS) compare
b0243 proA +0.4 2.6 gamma-glutamyl phosphate reductase (NCBI) compare
b4152 frdC +0.4 0.9 fumarate reductase subunit C (NCBI) compare
b4538 yoeF +0.4 0.5 hypothetical protein (RefSeq) compare
b2080 b2080 +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b1581 rspA +0.4 1.3 predicted dehydratase (NCBI) compare
b4695 ykgT +0.4 0.7 no description compare
b1187 fadR +0.4 1.7 fatty acid metabolism regulator (NCBI) compare
b2443 yffL +0.4 1.9 CPZ-55 prophage; predicted protein (NCBI) compare
b1808 yoaA +0.4 1.9 conserved protein with nucleoside triphosphate hydrolase domain (NCBI) compare
b0682 ybfN +0.4 1.9 predicted lipoprotein (NCBI) compare
b2061 wzb +0.4 0.9 protein-tyrosine phosphatase (NCBI) compare
b0655 gltI +0.4 1.3 glutamate and aspartate transporter subunit (NCBI) compare
b3228 sspB +0.4 1.6 ClpXP protease specificity-enhancing factor (NCBI) compare
b4373 rimI +0.4 0.8 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b1727 yniC +0.4 1.2 predicted hydrolase (NCBI) compare
b4277 yjgZ +0.4 2.3 KpLE2 phage-like element; predicted protein (NCBI) compare
b2674 nrdI +0.4 1.8 hypothetical protein (NCBI) compare
b2433 b2433 +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b1359 ydaU +0.4 1.6 Rac prophage; conserved protein (NCBI) compare
b1279 yciS +0.4 0.5 conserved inner membrane protein (NCBI) compare
b0447 ybaO +0.4 1.4 putative LRP-like transcriptional regulator (VIMSS) compare
b3250 mreC +0.4 1.0 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b2050 wcaI +0.4 1.9 predicted glycosyl transferase (NCBI) compare
b0450 glnK +0.4 1.2 nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB (NCBI) compare
b0753 ybgS +0.4 1.4 hypothetical protein (NCBI) compare
b1558 cspF +0.4 0.9 Qin prophage; cold shock protein (NCBI) compare
b0965 b0965 +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b2301 yfcF +0.4 1.6 predicted enzyme (NCBI) compare
b2883 guaD +0.4 2.2 guanine deaminase (NCBI) compare
b4641 yoeH +0.4 1.8 no description compare
b1521 uxaB +0.4 2.2 tagaturonate reductase (NCBI) compare
b1962 yedJ +0.4 1.8 predicted phosphohydrolase (NCBI) compare
b3119 tdcR +0.4 1.2 threonine dehydratase operon activator protein (VIMSS) compare
b3162 deaD +0.4 1.5 inducible ATP-independent RNA helicase (VIMSS) compare
b4144 yjeI +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b2662 gabT +0.4 2.2 4-aminobutyrate aminotransferase (NCBI) compare
b4656 yjhY +0.4 2.1 no description compare
b0641 rlpB +0.4 0.8 minor lipoprotein (NCBI) compare
b1379 hslJ +0.4 1.5 heat-inducible protein (NCBI) compare
b1656 sodB +0.4 0.8 superoxide dismutase, Fe (NCBI) compare
b1423 ydcJ +0.4 1.3 hypothetical protein (NCBI) compare
b2343 b2343 +0.4 0.8 orf, hypothetical protein (VIMSS) compare
b2281 nuoI +0.4 1.0 NADH dehydrogenase subunit I (NCBI) compare
b0219 yafV +0.4 0.9 predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI) compare
b1323 tyrR +0.4 1.7 DNA-binding transcriptional dual regulator, tyrosine-binding (NCBI) compare
b1336 ydaH +0.4 1.4 putative pump protein (transport) (VIMSS) compare
b2336 yfcS +0.4 2.3 predicted periplasmic pilus chaperone (NCBI) compare
b1731 cedA +0.4 1.2 cell division modulator (RefSeq) compare
b1725 yniA +0.4 1.7 predicted phosphotransferase/kinase (NCBI) compare
b0944 ycbF +0.4 2.2 putative chaperone (VIMSS) compare
b2432 yfeY +0.4 0.9 hypothetical protein (NCBI) compare
b4363 yjjB +0.4 1.2 conserved inner membrane protein (RefSeq) compare
b4543 ypaA +0.4 1.5 predicted protein (NCBI) compare
b0110 ampD +0.4 1.9 N-acetyl-anhydromuranmyl-L-alanine amidase (NCBI) compare
b1617 uidA +0.4 1.4 beta-D-glucuronidase (NCBI) compare
b1697 ydiQ +0.4 1.7 hypothetical protein (NCBI) compare
b1849 purT +0.4 1.9 phosphoribosylglycinamide formyltransferase 2 (NCBI) compare
b1949 fliQ +0.4 1.0 flagellar biosynthesis protein Q (NCBI) compare
b4014 aceB +0.4 2.5 malate synthase (NCBI) compare
b0010 yaaH +0.4 1.6 conserved inner membrane protein associated with acetate transport (NCBI) compare
b2535 csiE +0.4 2.7 orf, hypothetical protein (VIMSS) compare
b0805 fiu +0.4 2.3 predicted iron outer membrane transporter (NCBI) compare
b2785 rumA +0.4 2.7 23S rRNA (uracil-5-)-methyltransferase (NCBI) compare
b2454 eutJ +0.4 1.7 predicted chaperonin, ethanolamine utilization protein (NCBI) compare
b1219 ychN +0.4 1.3 hypothetical protein (NCBI) compare
b0549 ybcO +0.4 0.9 DLP12 prophage; predicted protein (NCBI) compare
b1765 ydjA +0.4 0.4 predicted oxidoreductase (NCBI) compare
b2095 gatZ +0.4 1.9 D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI) compare
b2105 yohL +0.4 1.5 hypothetical protein (NCBI) compare
b3057 bacA +0.4 1.8 undecaprenyl pyrophosphate phosphatase (NCBI) compare
b4566 yjhX +0.4 1.9 hypothetical protein (NCBI) compare
b4093 phnO +0.4 1.6 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b1261 trpB +0.4 0.8 tryptophan synthase subunit beta (NCBI) compare
b1661 cfa +0.4 1.5 cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI) compare
b4627 ykfL +0.4 1.2 no description compare
b4227 ytfQ +0.4 1.6 predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b0714 nei +0.4 1.2 endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI) compare
b3918 cdh +0.4 2.4 CDP-diacylglycerol pyrophosphatase (NCBI) compare
b2442 intZ +0.4 1.4 CPZ-55 prophage; predicted integrase (RefSeq) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Nalidixic acid sodium salt across organisms