Experiment set10IT077 for Escherichia coli BW25113

Compare to:

LB Anaerobic with Spiramycin 0.000125 mM

200 most important genes:

  gene name fitness t score description  
b0902 pflA -5.2 -6.2 pyruvate formate lyase activating enzyme 1 (NCBI) compare
b0463 acrA -4.5 -10.5 multidrug efflux system (NCBI) compare
b0462 acrB -4.4 -17.1 multidrug efflux system protein (NCBI) compare
b2512 yfgL -4.3 -5.1 protein assembly complex, lipoprotein component (NCBI) compare
b2188 yejM -4.3 -9.2 predicted hydrolase, inner membrane (NCBI) compare
b0903 pflB -4.3 -11.6 pyruvate formate lyase I (NCBI) compare
b2494 yfgC -4.1 -8.2 predicted peptidase (NCBI) compare
b3021 ygiT -3.9 -2.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b3035 tolC -3.8 -7.2 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b0957 ompA -3.8 -3.6 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b4509 ylcG -3.7 -3.5 DLP12 prophage; predicted protein (NCBI) compare
b2501 ppk -3.5 -8.9 polyphosphate kinase (NCBI) compare
b3842 rfaH -3.5 -4.7 transcriptional activator RfaH (NCBI) compare
b1334 fnr -3.4 -3.2 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b3632 rfaQ -3.4 -14.3 lipopolysaccharide core biosynthesis protein (NCBI) compare
b2531 iscR -3.3 -2.3 DNA-binding transcriptional repressor (NCBI) compare
b0677 nagA -3.3 -4.9 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b0222 lpcA -3.2 -2.2 phosphoheptose isomerase (NCBI) compare
b0178 hlpA -3.2 -4.8 periplasmic chaperone (NCBI) compare
b1830 prc -3.1 -3.7 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b3052 rfaE -3.1 -2.2 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3620 rfaF -3.1 -3.6 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3163 nlpI -3.1 -4.6 hypothetical protein (NCBI) compare
b0990 cspG -3.0 -2.0 DNA-binding transcriptional regulator (NCBI) compare
b4603 rseX -2.9 -1.4 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b1677 lpp -2.9 -2.8 murein lipoprotein (NCBI) compare
b4237 nrdG -2.9 -10.5 anaerobic ribonucleotide reductase activating protein (NCBI) compare
b4608 ryfC -2.9 -5.0 Novel sRNA, function unknown (RefSeq) compare
b3631 rfaG -2.9 -8.6 glucosyltransferase I (NCBI) compare
b0847 ybjL -2.9 -10.3 hypothetical protein (NCBI) compare
b1493 gadB -2.8 -1.4 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b4025 pgi -2.8 -7.9 glucose-6-phosphate isomerase (NCBI) compare
b0145 dksA -2.8 -2.7 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b4238 nrdD -2.8 -15.2 anaerobic ribonucleoside triphosphate reductase (NCBI) compare
b0053 surA -2.8 -2.7 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b1361 ydaW -2.7 -1.3 orf, hypothetical protein (VIMSS) compare
b0623 cspE -2.7 -2.2 cold shock protein E (NCBI) compare
b3621 rfaC -2.6 -2.6 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b2017 yefM -2.6 -2.5 orf, hypothetical protein (VIMSS) compare
b1236 galU -2.6 -2.5 glucose-1-phosphate uridylyltransferase (NCBI) compare
b0740 tolB -2.5 -2.9 translocation protein TolB precursor (NCBI) compare
b3630 rfaP -2.5 -6.0 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b2642 yfjW -2.4 -1.6 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b0739 tolA -2.3 -1.6 cell envelope integrity inner membrane protein TolA (NCBI) compare
b3619 rfaD -2.3 -2.2 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3627 rfaI -2.2 -6.1 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b2617 smpA -2.2 -3.4 small membrane protein A (VIMSS) compare
b2346 vacJ -2.1 -7.5 predicted lipoprotein (NCBI) compare
b0688 pgm -2.1 -2.9 phosphoglucomutase (NCBI) compare
b1482 osmC -2.1 -2.2 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b2064 asmA -2.0 -3.6 predicted assembly protein (NCBI) compare
b3199 yrbK -2.0 -3.0 hypothetical protein (NCBI) compare
b4701 sokX -2.0 -1.4 sok-related sRNA, function unknown (RefSeq) compare
b3195 yrbF -2.0 -9.8 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b1831 proQ -2.0 -4.2 putative solute/DNA competence effector (NCBI) compare
b4702 mgtL -2.0 -1.9 regulatory leader peptide for mgtA (NCBI) compare
b3644 yicC -1.9 -5.5 hypothetical protein (NCBI) compare
b1823 cspC -1.9 -2.2 stress protein, member of the CspA-family (NCBI) compare
b0730 mngR -1.9 -1.3 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b0737 tolQ -1.9 -1.8 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1863 ruvC -1.9 -1.8 Holliday junction resolvase (NCBI) compare
b2808 gcvA -1.8 -3.4 DNA-binding transcriptional dual regulator (NCBI) compare
b4220 ytfM -1.8 -9.4 predicted outer membrane protein and surface antigen (NCBI) compare
b0564 appY -1.8 -1.2 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b3405 ompR -1.8 -4.5 osmolarity response regulator (NCBI) compare
b0819 ybiS -1.8 -7.3 hypothetical protein (NCBI) compare
b2189 proL -1.8 -2.0 tRNA-Pro (NCBI) compare
b4480 hdfR -1.8 -5.3 transcriptional regulator HdfR (NCBI) compare
b4458 oxyS -1.8 -1.7 global regulatory RNA (NCBI) compare
b2572 rseA -1.8 -3.5 anti-sigma factor (NCBI) compare
b0741 pal -1.8 -3.3 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b3973 birA -1.8 -3.1 biotin--protein ligase (NCBI) compare
b0738 tolR -1.8 -1.2 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1286 rnb -1.7 -2.3 exoribonuclease II (NCBI) compare
b0802 ybiJ -1.7 -2.1 hypothetical protein (NCBI) compare
b3784 rfe -1.7 -9.8 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b1290 sapF -1.7 -1.6 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3341 rpsG -1.7 -1.6 30S ribosomal protein S7 (NCBI) compare
b4221 ytfN -1.7 -11.7 hypothetical protein (NCBI) compare
b3194 yrbE -1.7 -8.4 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0436 tig -1.7 -6.8 trigger factor (NCBI) compare
b3600 mtlD -1.7 -7.6 mannitol-1-phosphate 5-dehydrogenase (NCBI) compare
b2859 ygeO -1.6 -1.5 hypothetical protein (RefSeq) compare
b1685 ydiH -1.6 -1.6 predicted protein (RefSeq) compare
b4402 yjjY -1.6 -1.0 hypothetical protein (NCBI) compare
b1882 cheY -1.6 -0.8 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b3628 rfaB -1.6 -6.1 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b2571 rseB -1.6 -6.8 periplasmic negative regulator of sigmaE (NCBI) compare
b2553 glnB -1.6 -4.4 regulatory protein P-II for glutamine synthetase (NCBI) compare
b1291 sapD -1.5 -1.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4426 isrA -1.5 -2.1 unknown RNA (NCBI) compare
b3229 sspA -1.5 -2.0 stringent starvation protein A (NCBI) compare
b2506 yfgI -1.5 -1.7 hypothetical protein (NCBI) compare
b0058 rluA -1.5 -7.0 pseudouridine synthase for 23S rRNA (position 746) and tRNAphe(position 32) (NCBI) compare
b2174 b2174 -1.5 -5.5 orf, hypothetical protein (VIMSS) compare
b3649 rpoZ -1.5 -2.0 DNA-directed RNA polymerase subunit omega (NCBI) compare
b1620 malI -1.5 -2.3 DNA-binding transcriptional repressor (NCBI) compare
b1855 lpxM -1.4 -3.6 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b3609 secB -1.4 -3.3 export protein SecB (NCBI) compare
b3242 yhcR -1.4 -4.3 orf, hypothetical protein (VIMSS) compare
b0014 dnaK -1.4 -1.8 molecular chaperone DnaK (NCBI) compare
b0548 ninE -1.4 -1.8 DLP12 prophage; conserved protein (NCBI) compare
b1104 ycfL -1.3 -1.9 hypothetical protein (NCBI) compare
b4535 yniD -1.3 -1.2 predicted protein (RefSeq) compare
b3192 yrbC -1.3 -6.7 predicted ABC-type organic solvent transporter (NCBI) compare
b1476 fdnI -1.3 -1.1 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b4394 yjjX -1.3 -2.6 orf, hypothetical protein (VIMSS) compare
b1438 ydcQ -1.3 -2.5 predicted DNA-binding transcriptional regulator (RefSeq) compare
b2620 smpB -1.3 -1.2 SsrA-binding protein (NCBI) compare
b0239 frsA -1.3 -5.8 fermentation/respiration switch protein (NCBI) compare
b3930 menA -1.3 -8.3 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b0868 b0868 -1.3 -6.7 putative nucleotide di-P-sugar epimerase or dehydratase (VIMSS) compare
b3253 yhdH -1.3 -5.8 predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI) compare
b1241 adhE -1.3 -6.2 fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase (NCBI) compare
b3193 yrbD -1.3 -6.1 predicted ABC-type organic solvent transporter (NCBI) compare
b3443 yrhA -1.3 -1.3 orf, hypothetical protein (VIMSS) compare
b1961 dcm -1.3 -1.4 DNA cytosine methylase (NCBI) compare
b1950 fliR -1.3 -1.8 flagellar biosynthesis protein R (NCBI) compare
b4520 ymgF -1.3 -1.4 hypothetical protein (NCBI) compare
b1259 yciG -1.2 -1.4 orf, hypothetical protein (VIMSS) compare
b1818 manY -1.2 -2.1 mannose-specific enzyme IIC component of PTS (NCBI) compare
b0550 rusA -1.2 -2.5 DLP12 prophage; endonuclease RUS (NCBI) compare
b1332 ynaJ -1.2 -0.8 predicted inner membrane protein (NCBI) compare
b1249 cls -1.2 -4.7 cardiolipin synthetase (NCBI) compare
b2824 ygdB -1.2 -3.3 orf, hypothetical protein (VIMSS) compare
b1503 ydeR -1.2 -2.0 predicted fimbrial-like adhesin protein (NCBI) compare
b4438 cyaR -1.2 -2.2 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b1573 ydfC -1.2 -0.9 Qin prophage; predicted protein (RefSeq) compare
b1550 b1550 -1.2 -1.3 orf, hypothetical protein (VIMSS) compare
b3129 sohA -1.2 -2.9 predicted regulator (NCBI) compare
b2567 rnc -1.2 -0.7 ribonuclease III (NCBI) compare
b3858 yihD -1.2 -1.7 hypothetical protein (NCBI) compare
b3387 dam -1.2 -6.7 DNA adenine methylase (NCBI) compare
b0300 ykgA -1.2 -1.7 putative ARAC-type regulatory protein (VIMSS) compare
b0998 torD -1.2 -1.1 chaperone protein TorD (NCBI) compare
b2241 glpA -1.2 -6.7 sn-glycerol-3-phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)-binding (NCBI) compare
b3197 kdsD -1.2 -5.7 D-arabinose 5-phosphate isomerase (NCBI) compare
b4139 aspA -1.2 -6.1 aspartate ammonia-lyase (aspartase) (VIMSS) compare
b2258 b2258 -1.1 -1.2 putative transport/receptor protein (VIMSS) compare
b4676 yoaK -1.1 -0.7 expressed protein (RefSeq) compare
b4413 sokC -1.1 -1.4 regulatory antisense RNA (NCBI) compare
b0929 ompF -1.1 -3.1 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b3778 rep -1.1 -4.3 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b2085 b2085 -1.1 -2.4 orf, hypothetical protein (VIMSS) compare
b2376 ypdI -1.1 -2.0 predicted lipoprotein involved in colanic acid biosynthesis (NCBI) compare
b0866 ybjQ -1.1 -1.2 hypothetical protein (NCBI) compare
b3191 yrbB -1.1 -5.6 orf, hypothetical protein (VIMSS) compare
b3026 qseC -1.1 -1.7 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b0827 moeA -1.1 -3.4 molybdopterin biosynthesis protein (NCBI) compare
b1111 ycfQ -1.1 -2.1 orf, hypothetical protein (VIMSS) compare
b4000 hupA -1.1 -3.2 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b2369 evgA -1.1 -1.7 DNA-binding response regulator in two-component regulatory system with EvgS (NCBI) compare
b3148 yraN -1.1 -3.0 hypothetical protein (NCBI) compare
b0364 yaiS -1.1 -2.0 orf, hypothetical protein (VIMSS) compare
b0200 gmhB -1.1 -0.7 hypothetical protein (NCBI) compare
b2144 sanA -1.1 -6.2 hypothetical protein (NCBI) compare
b1085 b1085 -1.1 -1.1 orf, hypothetical protein (VIMSS) compare
b2243 glpC -1.1 -4.0 sn-glycerol-3-phosphate dehydrogenase (anaerobic), small subunit (NCBI) compare
b1548 nohA -1.1 -1.0 Qin prophage; predicted packaging protein (RefSeq) compare
b1566 flxA -1.0 -2.1 Qin prophage; predicted protein (NCBI) compare
b0082 mraW -1.0 -2.1 S-adenosyl-methyltransferase (NCBI) compare
b4511 ybdZ -1.0 -2.4 hypothetical protein (NCBI) compare
b3764 yifE -1.0 -3.2 hypothetical protein (NCBI) compare
b4201 priB -1.0 -1.8 primosomal replication protein N (NCBI) compare
b0232 yafN -1.0 -0.6 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b2317 dedA -1.0 -2.6 conserved inner membrane protein (NCBI) compare
b4179 vacB -1.0 -3.3 putative enzyme (VIMSS) compare
b0617 citD -1.0 -2.0 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b0049 apaH -1.0 -1.7 diadenosinetetraphosphatase (NCBI) compare
b0729 sucD -1.0 -1.9 succinyl-CoA synthetase subunit alpha (NCBI) compare
b1969 yedW -1.0 -2.4 putative 2-component transcriptional regulator (VIMSS) compare
b3794 rffM -1.0 -4.5 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b3636 rpmG -1.0 -1.4 50S ribosomal protein L33 (NCBI) compare
b2820 recB -1.0 -0.7 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b3839 tatC -1.0 -4.1 TatABCE protein translocation system subunit (NCBI) compare
b0092 ddlB -1.0 -3.7 D-alanylalanine synthetase (NCBI) compare
b4545 ypdJ -1.0 -1.3 no description compare
b3961 oxyR -1.0 -5.3 DNA-binding transcriptional dual regulator (NCBI) compare
b1183 umuD -1.0 -1.6 DNA polymerase V, subunit D (NCBI) compare
b2576 srmB -1.0 -1.9 ATP-dependent RNA helicase (NCBI) compare
b3916 pfkA -1.0 -1.1 6-phosphofructokinase (NCBI) compare
b3932 hslV -1.0 -5.8 ATP-dependent protease peptidase subunit (NCBI) compare
b3931 hslU -1.0 -6.1 ATP-dependent protease ATP-binding subunit (NCBI) compare
b2689 yqaA -1.0 -2.4 conserved inner membrane protein (NCBI) compare
b4436 ryeC -1.0 -1.4 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b3469 zntA -1.0 -4.2 zinc, cobalt and lead efflux system (NCBI) compare
b4559 yjdO -0.9 -1.7 hypothetical protein (NCBI) compare
b1604 ydgH -0.9 -2.2 hypothetical protein (NCBI) compare
b4146 yjeK -0.9 -0.6 predicted lysine aminomutase (NCBI) compare
b1626 b1626 -0.9 -3.8 orf, hypothetical protein (VIMSS) compare
b3466 yhhL -0.9 -3.1 conserved inner membrane protein (NCBI) compare
b0904 focA -0.9 -4.0 formate transporter (NCBI) compare
b4640 yoeG -0.9 -1.1 no description compare
b2830 nudH -0.9 -1.0 dinucleoside polyphosphate hydrolase (NCBI) compare
b2165 yeiN -0.9 -3.1 hypothetical protein (NCBI) compare
b2215 ompC -0.9 -5.0 outer membrane porin protein C (NCBI) compare
b2935 tktA -0.9 -0.9 transketolase 1, thiamin-binding (NCBI) compare
b1107 nagZ -0.9 -2.8 beta-hexosaminidase (NCBI) compare
b0814 ompX -0.9 -2.5 outer membrane protein X (NCBI) compare
b2363 yfdT -0.9 -1.7 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare


Specific Phenotypes

For 12 genes in this experiment

For stress Spiramycin in Escherichia coli BW25113

For stress Spiramycin across organisms