Experiment set10IT077 for Escherichia coli BW25113

Compare to:

LB Anaerobic with Spiramycin 0.000125 mM

200 most detrimental genes:

  gene name fitness t score description  
b0464 acrR +4.6 27.8 DNA-binding transcriptional repressor (NCBI) compare
b3169 nusA +3.9 8.8 transcription elongation factor NusA (NCBI) compare
b0439 lon +3.2 11.5 DNA-binding ATP-dependent protease La (NCBI) compare
b3390 aroK +3.2 6.7 shikimate kinase I (NCBI) compare
b0955 ycbZ +3.1 20.4 predicted peptidase (NCBI) compare
b1187 fadR +3.0 9.8 fatty acid metabolism regulator (NCBI) compare
b3493 pitA +2.8 18.8 phosphate transporter, low-affinity (NCBI) compare
b1049 mdoH +2.2 15.8 glucosyltransferase MdoH (NCBI) compare
b1048 mdoG +2.1 11.8 glucan biosynthesis protein, periplasmic (NCBI) compare
b3205 yhbJ +2.1 8.8 hypothetical protein (NCBI) compare
b3979 thrT +2.0 1.2 tRNA-Thr (NCBI) compare
b3806 cyaA +1.9 8.0 adenylate cyclase (NCBI) compare
b1530 marR +1.9 5.1 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b0912 ihfB +1.7 6.5 integration host factor subunit beta (NCBI) compare
b2688 gshA +1.7 9.4 glutamate--cysteine ligase (NCBI) compare
b2318 truA +1.7 4.8 tRNA pseudouridine synthase A (NCBI) compare
b2175 spr +1.6 5.9 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b4441 glmY +1.6 4.1 sRNA activator of glmS mRNA (RefSeq) compare
b2517 yfgB +1.6 9.7 predicted enzyme (NCBI) conserved
b2684 mprA +1.6 4.5 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b4241 treR +1.6 12.6 trehalose repressor (NCBI) compare
b0125 hpt +1.5 6.5 hypoxanthine phosphoribosyltransferase (VIMSS) compare
b0685 ybfE +1.4 3.7 orf, hypothetical protein (VIMSS) compare
b1095 fabF +1.3 8.1 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b1628 rsxB +1.3 3.8 electron transport complex protein RnfB (NCBI) compare
b0209 yafD +1.3 6.8 hypothetical protein (NCBI) compare
b0926 ycbK +1.3 5.0 hypothetical protein (NCBI) compare
b3977 tyrU +1.2 1.3 tRNA-Tyr (NCBI) compare
b3688 yidQ +1.2 5.7 orf, hypothetical protein (VIMSS) compare
b4042 dgkA +1.2 2.6 diacylglycerol kinase (NCBI) compare
b2096 gatY +1.2 5.1 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b2814 metZ +1.2 2.2 tRNA-Met (NCBI) compare
b2516 yfgA +1.1 7.5 hypothetical protein (NCBI) compare
b2614 grpE +1.1 2.0 heat shock protein (NCBI) compare
b2939 yqgB +1.1 1.7 predicted protein (RefSeq) compare
b1865 nudB +1.1 1.9 dATP pyrophosphohydrolase (NCBI) compare
b3204 ptsN +1.1 3.3 sugar-specific enzyme IIA component of PTS (NCBI) compare
b3781 trxA +1.1 3.5 thioredoxin 1 (VIMSS) compare
b1582 ynfA +1.1 2.6 hypothetical protein (NCBI) compare
b4171 miaA +1.1 3.0 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b4063 soxR +1.1 3.0 DNA-binding transcriptional dual regulator, Fe-S center for redox-sensing (NCBI) compare
b2951 yggS +1.1 4.4 predicted enzyme (NCBI) compare
b2058 wcaB +1.0 1.4 predicted acyl transferase (NCBI) compare
b3071 yqjI +1.0 3.7 predicted transcriptional regulator (NCBI) compare
b1263 trpD +1.0 2.7 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b1684 sufA +1.0 2.5 iron-sulfur cluster assembly scaffold protein (NCBI) compare
b1712 ihfA +1.0 1.4 integration host factor subunit alpha (NCBI) compare
b0407 yajC +1.0 2.6 preprotein translocase subunit YajC (NCBI) compare
b4699 fnrS +1.0 1.1 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b4041 plsB +1.0 2.4 glycerol-3-phosphate acyltransferase (VIMSS) compare
b2947 gshB +1.0 3.8 glutathione synthetase (NCBI) compare
b0858 ybjO +0.9 2.5 predicted inner membrane protein (NCBI) compare
b3792 wzxE +0.9 5.7 O-antigen translocase (NCBI) compare
b0624 crcB +0.9 1.3 camphor resistance protein CrcB (NCBI) compare
b4416 rybA +0.9 1.7 unknown RNA (NCBI) compare
b1848 yebG +0.9 1.7 conserved protein regulated by LexA (NCBI) compare
b2281 nuoI +0.9 2.3 NADH dehydrogenase subunit I (NCBI) compare
b4572 ylbE +0.9 2.6 no description compare
b4529 ydbJ +0.9 1.2 hypothetical protein (NCBI) compare
b4151 frdD +0.9 3.1 fumarate reductase subunit D (NCBI) compare
b2187 yejL +0.9 2.9 hypothetical protein (NCBI) compare
b4630 ykgP +0.9 2.6 no description compare
b1805 fadD +0.8 5.2 acyl-CoA synthase (NCBI) compare
b1667 ydhR +0.8 2.5 hypothetical protein (NCBI) compare
b2285 nuoE +0.8 2.1 NADH dehydrogenase subunit E (NCBI) compare
b2450 yffS +0.8 1.7 CPZ-55 prophage; predicted protein (RefSeq) compare
b3180 yhbY +0.8 4.1 predicted RNA-binding protein (NCBI) compare
b2263 yfbB +0.8 2.8 predicted peptidase (NCBI) compare
b2597 yfiA +0.8 1.8 cold shock protein associated with 30S ribosomal subunit (NCBI) compare
b1150 ymfR +0.8 1.0 e14 prophage; predicted protein (NCBI) compare
b3057 bacA +0.8 5.0 undecaprenyl pyrophosphate phosphatase (NCBI) compare
b3388 damX +0.8 6.6 hypothetical protein (NCBI) compare
b2221 atoD +0.8 2.9 acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI) compare
b4174 hflK +0.8 5.0 modulator for HflB protease specific for phage lambda cII repressor (NCBI) compare
b2279 nuoK +0.8 1.3 NADH dehydrogenase subunit K (NCBI) compare
b2708 gutQ +0.7 3.1 orf, hypothetical protein (VIMSS) compare
b4431 rprA +0.7 1.5 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b4301 sgcE +0.7 4.4 ribulose-phosphate 3-epimerase (NCBI) compare
b2906 visC +0.7 3.6 hypothetical protein (NCBI) compare
b3926 glpK +0.7 5.1 glycerol kinase (NCBI) compare
b1070 flgN +0.7 1.1 export chaperone for FlgK and FlgL (NCBI) compare
b4527 b1354 +0.7 2.5 orf, hypothetical protein (VIMSS) compare
b3181 greA +0.7 2.9 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b2289 lrhA +0.7 4.7 DNA-binding transcriptional repressor of flagellar, motility and chemotaxis genes (NCBI) compare
b0920 ycbC +0.7 3.3 conserved inner membrane protein (NCBI) compare
b2134 pbpG +0.7 4.2 penicillin-binding protein 7 (VIMSS) compare
b2683 ygaH +0.7 3.7 predicted inner membrane protein (NCBI) compare
b2492 focB +0.7 2.2 predicted formate transporter (NCBI) compare
b0212 gloB +0.7 2.3 predicted hydroxyacylglutathione hydrolase (NCBI) compare
b0991 ymcE +0.7 2.2 cold shock gene (NCBI) compare
b0428 cyoE +0.7 3.0 protoheme IX farnesyltransferase (NCBI) compare
b3398 yrfF +0.7 2.6 predicted inner membrane protein (NCBI) compare
b0348 mhpB +0.7 1.0 2,3-dihydroxyphenylpropionate 1,2-dioxygenase (NCBI) compare
b1143 ymfI +0.7 1.6 orf, hypothetical protein (VIMSS) compare
b3009 yghB +0.7 4.1 conserved inner membrane protein (NCBI) compare
b1242 ychE +0.7 2.0 predicted inner membrane protein (NCBI) compare
b1846 yebE +0.7 2.2 hypothetical protein (NCBI) compare
b2911 ssrS +0.7 1.1 6S regulatory RNA (NCBI) compare
b4692 oweE +0.7 2.0 no description compare
b1630 rsxD +0.7 3.4 electron transport complex protein RnfD (NCBI) compare
b1090 plsX +0.7 1.8 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b4631 ykgQ +0.7 0.7 no description compare
b1185 dsbB +0.7 1.8 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b1064 grxB +0.6 3.2 glutaredoxin 2 (Grx2) (NCBI) compare
b2618 b2618 +0.6 2.7 orf, hypothetical protein (VIMSS) compare
b1134 ymfB +0.6 1.2 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b2240 glpT +0.6 4.5 sn-glycerol-3-phosphate transporter (NCBI) compare
b0220 ivy +0.6 2.3 inhibitor of vertebrate C-lysozyme (NCBI) compare
b1067 yceH +0.6 2.8 hypothetical protein (NCBI) compare
b2432 yfeY +0.6 0.9 hypothetical protein (NCBI) compare
b1384 feaR +0.6 3.8 DNA-binding transcriptional dual regulator (NCBI) compare
b1349 recT +0.6 3.0 Rac prophage; recombination and repair protein (NCBI) compare
b3258 panF +0.6 4.2 sodium/pantothenate symporter (VIMSS) compare
b2128 yehW +0.6 2.8 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b2195 ccmG +0.6 2.0 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b1478 adhP +0.6 2.8 alcohol dehydrogenase (VIMSS) compare
b4656 yjhY +0.6 3.0 no description compare
b3500 gor +0.6 1.1 glutathione reductase (NCBI) compare
b3101 yqjF +0.6 2.0 orf, hypothetical protein (VIMSS) compare
b1671 ydhX +0.6 1.7 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b4370 leuQ +0.6 1.8 tRNA-Leu (NCBI) compare
b3611 yibN +0.6 3.0 predicted rhodanese-related sulfurtransferase (NCBI) compare
b2283 nuoG +0.6 3.6 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b2276 nuoN +0.6 2.3 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b2216 rcsD +0.6 3.9 phosphotransfer intermediate protein in two-component regulatory system with RcsBC (NCBI) compare
b1520 b1520 +0.6 1.8 orf, hypothetical protein (VIMSS) compare
b1867 yecD +0.6 3.1 orf, hypothetical protein (VIMSS) compare
b2145 yeiS +0.6 2.2 predicted inner membrane protein (NCBI) compare
b1731 cedA +0.6 2.0 cell division modulator (RefSeq) compare
b2659 b2659 +0.6 0.9 orf, hypothetical protein (VIMSS) compare
b4093 phnO +0.6 3.3 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b2154 yeiG +0.6 1.4 predicted esterase (NCBI) compare
b2542 hcaD +0.6 2.7 phenylpropionate dioxygenase, ferredoxin reductase subunit (NCBI) compare
b3257 yhdT +0.6 2.0 conserved inner membrane protein (NCBI) compare
b1949 fliQ +0.6 0.9 flagellar biosynthesis protein Q (NCBI) compare
b0982 yccY +0.6 2.7 putative phosphatase (VIMSS) compare
b2682 ygaZ +0.6 2.3 predicted transporter (NCBI) compare
b0714 nei +0.6 2.0 endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI) compare
b0845 ybjJ +0.6 3.3 predicted transporter (NCBI) compare
b4409 blr +0.6 2.6 beta-lactam resistance membrane protein (NCBI) compare
b3113 yhaR +0.6 2.9 orf, hypothetical protein (VIMSS) compare
b0791 ybhQ +0.6 1.9 predicted inner membrane protein (NCBI) compare
b2918 argK +0.6 2.3 arginine/ornithine transport system ATPase (NCBI) compare
b0310 ykgH +0.6 0.8 predicted inner membrane protein (NCBI) compare
b3844 fre +0.6 3.0 NAD(P)H-flavin reductase (NCBI) compare
b4595 yciY +0.6 1.0 hypothetical protein (RefSeq) compare
b0493 ybbO +0.6 3.1 short chain dehydrogenase (NCBI) compare
b0367 tauC +0.6 2.4 taurine transporter subunit (NCBI) compare
b1901 araF +0.6 2.1 L-arabinose transporter subunit (NCBI) compare
b1289 ycjD +0.6 2.0 hypothetical protein (NCBI) compare
b1919 yedO +0.6 1.8 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b1558 cspF +0.6 1.3 Qin prophage; cold shock protein (NCBI) compare
b1661 cfa +0.6 1.9 cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI) compare
b1186 nhaB +0.6 3.0 NhaB sodium/proton transporter (Katherine Huang) compare
b4325 yjiC +0.6 1.6 orf, hypothetical protein (VIMSS) compare
b1917 yecC +0.6 1.9 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b4698 mgrR +0.6 1.3 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b2280 nuoJ +0.6 2.7 NADH dehydrogenase subunit J (NCBI) compare
b1553 ydfP +0.6 1.1 Qin prophage; conserved protein (NCBI) compare
b2350 yfdG +0.6 3.2 CPS-53 (KpLE1) prophage; bactoprenol-linked glucose translocase (flippase) (NCBI) compare
b1842 holE +0.6 0.9 DNA polymerase III, theta subunit (NCBI) compare
b2946 yggJ +0.5 2.6 orf, hypothetical protein (VIMSS) compare
b3029 ygiN +0.5 1.1 quinol monooxygenase (NCBI) compare
b3871 bipA +0.5 3.8 GTP-binding protein (NCBI) compare
b1845 ptrB +0.5 3.0 protease II (NCBI) compare
b1321 ycjX +0.5 2.1 conserved protein with nucleoside triphosphate hydrolase domain (NCBI) compare
b0869 ybjT +0.5 2.9 putative dTDP-glucose enzyme (VIMSS) compare
b0303 ykgI +0.5 0.5 orf, hypothetical protein (VIMSS) compare
b1563 relE +0.5 1.0 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b0418 pgpA +0.5 2.4 phosphatidylglycerophosphatase A (NCBI) compare
b0143 pcnB +0.5 2.8 poly(A) polymerase I (VIMSS) compare
b0881 clpS +0.5 0.9 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b2768 ygcP +0.5 1.2 predicted anti-terminator regulatory protein (NCBI) compare
b2836 aas +0.5 3.8 2-acyl-glycerophospho-ethanolamine acyltransferase (NCBI) compare
b1565 ydfV +0.5 2.0 Qin prophage; predicted protein (NCBI) compare
b4558 yifL +0.5 2.3 predicted lipoprotein (NCBI) compare
b2835 ygeD +0.5 3.0 predicted inner membrane protein (NCBI) compare
b1248 b1248 +0.5 1.5 orf, hypothetical protein (VIMSS) compare
b3962 udhA +0.5 2.9 putative oxidoreductase (VIMSS) compare
b1343 dbpA +0.5 2.3 ATP-dependent RNA helicase, specific for 23S rRNA (NCBI) compare
b2061 wzb +0.5 1.1 protein-tyrosine phosphatase (NCBI) compare
b3425 glpE +0.5 2.6 thiosulfate sulfurtransferase (NCBI) compare
b3250 mreC +0.5 0.5 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b0384 psiF +0.5 1.6 induced by phosphate starvation (VIMSS) compare
b0594 entE +0.5 2.8 2,3-dihydroxybenzoate-AMP ligase component of enterobactin synthase multienzyme complex (NCBI) compare
b1581 rspA +0.5 1.7 predicted dehydratase (NCBI) compare
b2448 yffQ +0.5 1.6 CPZ-55 prophage; predicted protein (RefSeq) compare
b2725 hycA +0.5 1.9 regulator of the transcriptional regulator FhlA (NCBI) compare
b0906 ycaP +0.5 2.6 conserved inner membrane protein (NCBI) compare
b3465 yhhF +0.5 2.4 predicted methyltransferase (NCBI) compare
b4552 yrhC +0.5 0.5 no description compare
b4521 ycgI +0.5 1.5 hypothetical protein (NCBI) compare
b4176 yjeT +0.5 2.0 conserved inner membrane protein (NCBI) compare
b0015 dnaJ +0.5 3.1 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b1627 rsxA +0.5 1.5 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b4513 kdpF +0.5 1.7 potassium ion accessory transporter subunit (NCBI) compare
b1475 fdnH +0.5 2.6 formate dehydrogenase-N, Fe-S (beta) subunit, nitrate-inducible (NCBI) compare
b1706 ydiU +0.5 2.8 hypothetical protein (NCBI) compare
b1152 ymfP +0.5 2.3 orf, hypothetical protein (VIMSS) compare
b1220 ychO +0.5 1.3 predicted invasin (RefSeq) compare


Specific Phenotypes

For 12 genes in this experiment

For stress Spiramycin in Escherichia coli BW25113

For stress Spiramycin across organisms